Clinical Observation on Rongjin Tongbi Decoction in Treating Sciatica Caused by Lumbar Intervertebral Disc Herniation with Liver and Kidney Deficiency Type / 中国实验方剂学杂志

ObjectiveTo evaluate the efficacy and safety of Rongjin Tongbi decoction on sciatica caused by lumbar intervertebral disc herniation with liver and kidney deficiency type based on a randomized controlled study. MethodFrom January 2019 to July 2022， 90 patients in the department of Traditional Chinese medicine（TCM） of Beijing Jishuitan Hospital who met the inclusion criteria were selected and divided into two groups according to the random number table， with 45 patients in each group. During the study， 19 cases dropped out， with 41 cases included in the final observation group and 30 cases in the control group. The observation group was given Rongjin Tongbi decoction orally， and the control group was given Loxoprofen Sodium Tablets orally for 28 days. The differences in the visual analog scale （VAS） scores， the Japanese Orthopaedic Association （JOA） scores， activities of daily living （ADL） assessments， TCM clinical symptoms， subjective symptoms， and clinical signs scores between two groups before and after treatment were observed. Liver and kidney functions and gastrointestinal adverse reactions were detected for safety evaluation. ResultBefore treatment， there was no statistically significant difference in scores between the observation group and the control group. After treatment， the absolute values of the differences in VAS and ADL scores in the observation group were higher than those in the control group （P<0.05）. There was no statistically significant difference in the absolute value of the difference in JOA scores between two groups. The absolute value of the difference in TCM clinical symptom scores in the observation group was higher than that in the control group （P<0.01）. There was no statistically significant difference in the absolute values of the differences in subjective symptom and clinical sign scores between two groups. The levels of liver and kidney function indicators in both groups before and after the experiment were normal， and there was no significant difference in gastrointestinal reactions. ConclusionRongjin Tongbi decoction can significantly improve the symptoms of sciatica patients caused by lumbar intervertebral disc herniation with liver and kidney deficiency type. After treatment， the patients exhibited significant improvements in pain， activity and other aspects， and it is proven to be safe and reliable， which is conducive to the recovery of physical function.

Gut microbes in cardiovascular diseases and their potential therapeutic applications

Microbial ecosystem comprises a complex community in which bacteria interact with each other. The potential roles of the intestinal microbiome play in human health have gained considerable attention. The imbalance of gut microbial community has been looked to multiple chronic diseases. Cardiovascular diseases (CVDs) are leading causes of morbidity worldwide and are influenced by genetic and environmental factors. Recent advances have provided scientific evidence that CVD may also be attributed to gut microbiome. In this review, we highlight the complex interplay between microbes, their metabolites, and the potential influence on the generation and development of CVDs. The therapeutic potential of using intestinal microbiomes to treat CVD is also discussed. It is quite possible that gut microbes may be used for clinical treatments of CVD in the near future.

Application of New Single-cell Analysis Technology——Cytometry by Time-of-flight in Treatment of Leukemia / 肿瘤防治研究

Cytometry by time-of-flight (CyTOF) is an innovative single-cell analysis technique combining with mass spectrometry principles and flow cytometry. In recent years, CyTOF has been widely used in the study of leukemia treatment for its advantages of multi-parameter, high-throughput, compensation without calculation and diversified data analysis. It can accurately determine intracellular element contents and analyze the changes of unique phenotype and complex signaling pathways of leukemia cells at the single-cell level, to study the mechanism of action of anti-leukemia drugs, discover the potential therapeutic targets of leukemia, assess the therapeutic response and deeply understand the mechanism of leukemia relapse and drug resistance. This article reviews the research progress of CyTOF principle, characteristics, advantages and its application in leukemia treatment.

Pay attention to the outcome and the end point of anti-vascular endothelial growth factor therapy for retinopathy of prematurity / 中华眼底病杂志

In the expert consensus published by the Pediatrics in 2013,it was first proposed that anti-VEGF drugs can be considered for retinopathy ofprematurity (ROP) with stage 3,zone Ⅰ with plus disease.However,there are many problems worth the attention of ophthalmologists,including the advantages and disadvantages of anti-VEGF therapy compared with traditional laser therapy,systemic and ocular complications after anti-VEGF therapy,and what indicators are the end points of anti-VEGF therapy.Combined with this consensus and numerous research findings,we recommend that the first treatment for anti-VEGF or laser therapy should be considered from disease control effects.For the threshold and pre-threshold lesions,the effect of anti-VEGF therapy for zone Ⅱ lesions is better than that for zone Ⅰ lesions and the single-time effective rate is high.So,it is suggested that anti-VEGF therapy should be preferred for the first treatment.The choice of repeat treatment should be considered from the final retinal structure and functional prognosis.Laser therapy is advisable for the abnormal vascular regression slower and abnormalities in the posterior pole.It can reduce the number of reexaminations and prolong the interval between re-examinations.However,the premature use of laser has an inevitable effect on peripheral vision field.Excluding the above problems,supplemental therapy can still choose anti-VEGF therapy again.Most of the children with twice anti-VEGF therapy are sufficient to control the disease.Anti-VEGF therapy should be terminated when there are signs such as plus regression,threshold or pre-threshold lesions controlled without recurrence,peripheral vascularization,etc.

Review of prognostic factors of anti-vascular endothelial growth factor therapy on choroidal neovascularization secondary to pathological myopia / 中华眼底病杂志

For choroidal neovascularization (CNV) secondary to pathological myopia, intravitreal injection of anti-VEGF has been widely used in clinic and achieved good outcome. However, due to the differences in the demographic characteristics, stages of disease progression and treatment procedure of CNV, the prognosis of the disease is variable. Complete ellipsoid band, smaller baseline choroidal neovascularization and better baseline vision are important predictors of good outcome of anti-vascular endothelial growth factor treatment. Chorioretinal atrophy or complications related to pathologic myopia indicate a poor prognosis. The influence of age, race, previous photodynamic therapy and early treatment on the prognosis of treatment need to be further studied.

Ethosomes as Novel Drug Delivery System: A Review.

The ethosomes are vesicular carrier comprise of hydroalcoholic or hydro/alcoholic/glycolic phospholipid in which the concentration of alcohols or their combination is relatively high. To provide continuous drug infusion through an intact skin, several transdermal therapeutic systems have been developed for topical application onto the intact skin surface to control the delivery of drug and its subsequent permeation through the skin tissue. Transdermal route is promising alternative to drug delivery for systemic effect. An attempt was made to formulate the highly efficient ethosomal drug delivery system and enalapril meleate is used as model drug. The following conclusion are drown from the result and discussion described in the previous chapter. Liposomal formulation was also prepared by the thin film hydration method. The techniques used were simple and reproducible. The prepared ethosomes were spherical and discrete in shape. The size of vesicles were found to be in the range of 3.26-5.79 tim,o.716-1.3o1 tim and 5.32 tim for unsonicated ethosomes, sonicated ethosomes and liposomes respectively. However ethosomes prepared by sonication method were more uniform and smaller in size, which is essential for skin permeation. While comparing the entrapment efficiency, ethosomes containing 3o% w/w ethanol and prepared by sonication showed highest value with respect to all other formulation, so it is concluded ethosomes prepared by sonication and containing 3o% w/w ethanol as the best formulation considering all other aspects. The highest value of transdermal flux for sonicated ethosomes containing 3o% w/w ethanol is the indication of complete and rapid penetration through the skin may be because of tiny vesicular size.

The main potentialities of resveratrol for drug delivery systems

Resveratrol (RSV), a natural polyphenol found in grapes, was found to be effective in the prevention and therapy of several diseases, however, it does have unfavourable physicochemical properties. In this context, an increasing number of studies have aimed at developing novel therapeutic systems for its delivery to overcome these disadvantages. This review focuses on the mechanisms of action and therapeutic applications. Finally, it also describes some tested formulations for RSV administration, controlled release and targeting, developed with the purpose of increasing RSV bioavailability.

O resveratrol (RSV) é um polifenol natural encontrado nas uvas, que se mostrou eficaz na prevenção e terapia de várias doenças. No entanto, apresenta propriedades físico-químicas desfavoráveis. Neste contexto, um número cada vez maior de estudos visando ao desenvolvimento de novos sistemas terapêuticos para a sua liberação vem sendo desenvolvido no sentido de ultrapassar estas desvantagens. Esta revisão discorre sobre os mecanismos de ação e aplicações terapêuticas do RSV. Finalmente, são abordadas algumas formulações de liberação controlada e vetorizada, testadas para administração do RSV, desenvolvidas com a finalidade de aumentar a biodisponibilidade do RSV.

Potential roles and therapeutic applications of P2 X7 receptor in inflammation and pain / 中国药理学通报

The P2X7 receptor (P2X7 R)is a nonselective cation channel activated by extracellular ATP,and it may trigger the se-cretion of several proinflammatory substances,such as IL-1β, IL-18,TNF-α,and nitric oxide.Several preclinical studies have demonstrated that this receptor participates in inflammation and pain mechanisms,and compounds that modulate the function of this receptor show potential as new anti-inflammatory medicines. Therefore,P2X7 receptors play particularly important physiologi-cal roles and have potential clinical application in inflammation and pain,which proves it a therapeutic target for pain manage-ment.

Isolation and purification of bromelain from waste peel of pineapple for therapeutic application

The aim of this work was to isolate and purify bromelain extracted from the pineapple peel by ammonium sulfate precipitation (40-80%), followed by desalting and freeze-drying with a 75% activity recovery and 2.2 fold increased specific activity. Ion exchange chromatography on DEAE-Sepharose was able to separate the polysaccharides from the enzyme, which was recovered in the elution step, maintaining its enzymatic activity. The batch adsorption of bromelain was evaluated in terms of total protein and enzymatic activity using Langmuir and Langmuir-Freundlich models. Results showed that the process could be suitable for the recovery and purification of the enzyme, maintaining its specific activity.

Aplicación terapéutica del Aloe vera L. en Odontología / Therapeutic application of Aloe vera in dentistry

El Aloe vera L. es una planta de la familia Asphodelaceae usada de manera empírica desde hace más de 4000 años, para múltiples usos medicinales. En 1936, se publica la primera aplicación medicinal, lo que marca el inicio de su estudio científico riguroso, validando acciones farmacológicas antimicrobiana, antiinflamatoria, antioxidante y efectos cicatrizante, protector gástrico, antineoplásico, hipoglucemiante y hepatoprotector, entre otros. Por su acción antibacteriana, antiinflamatoria y cicatrizante se ha investigado su uso en el tratamiento de la enfermedad periodontal; en la prevención de gingivitis, caries dental y mucositis; así como en la formación de puente dentinario, en la regeneración de tejido óseo y mucoso, y en patologías como la fibrosis múltiple y el liquen plano bucal, entre otros. Esta revisión bibliográfica presenta una breve descripción botánica y de la composición química de la planta, así como de sus acciones farmacológicas, con énfasis en su aplicación Odontológica, donde las investigaciones en esta área son escasas, además de no contar en Venezuela con productos para tal fin. Su uso como biomaterial puede ser una alternativa a los fármacos convencionales en el tratamiento de la enfermedad periodontal de alta prevalencia mundial, en la pulpectomía, así como cicatrizante post exodoncia y en la prevención de caries dental, gingivitis y mucositis, basado en los resultados de las investigaciones reseñadas.

Aloe vera L: is a plant of the family Asphodelaceae, which is used in herbal medicine empirically for more than 4000 years ago, because of its many medicinal uses. The first research of its medicinal use was in 1936, where he began his rigorous scientific study, validating their pharmacological actions antimicrobial, anti-inflammatory, antioxidant and healing effects, gastric protection, anticancer, hypoglycemic and hepatoprotective. By its antibacterial action, anti-inflammatory and healing was investigated for use in the treatment of periodontal disease in the prevention of gingivitis, dental caries and mucositis; in dentinal bridge formation, and the regeneration of bone tissue and mucosal and in diseases such as multiple fibrosis and oral lichen planus, among others. Thus, this article presents an literature review with a This literature review provides a brief botanical description and chemical composition of the plant, and its pharmacological actions, with emphasis on its application Dentistry, besides not having to Venezuela with products such end. Its use as a biomaterial can be an alternative to conventional treatment of periodontal disease highly prevalent worldwide in pulpotomy and post extraction healing and prevention of dental caries, gingivitis and mucositis based on research results outlined.

Lectins: Proteins with Diverse Applications.

Lectins are proteins that bind to carbohydrates and sugar containing substances in a specific and reversible way or precipitate glycoconjugates. These heterogeneous class of carbohydrate-binding proteins or glycoproteins of non-immune origin are capable of specific recognition of, and reversible binding to, carbohydrates without altering their covalent structure. Lectins are found in a diversity of organisms and possess the ability to agglutinate erythrocytes with known carbohydrate specificity since they have at least one non-catalytic domain that binds reversibly to specific monosaccharides or oligosaccharides. This review aims to highlight the applications of lectins in various fields of biology. Lectins are isolated from their natural sources by chromatographic procedures with various modulations to increase their production. The yields of animal lectins are usually low compared with the yields of plant lectins such as legume lectins, which form a major source of these proteins. Lectins manifest a diversity of activities including anti-insect activities, antitumor, immunomodulatory, antimicrobial and HIV-1 reverse transcriptase inhibitory, which may find applications in many therapeutic areas. A small number of lectins demonstrate anti-parasitic activities.

MICROFABRICATED NANOCHANNELS: NEW TOOLS FOR MOLECULAR MOTION CONTROL / MICRO Y NANOCANALES INTEGRADOS: NUEVAS HERRAMIENTAS PARA CONTROL DE MOVIMIENTO MOLECULAR

This paper presents a review of work on the fabrication and use of nanochannels in silicon and polymers for the control of molecular transport. The method of Sacrificial Layer Lithography is reviewed and demonstrated for silicon and polymers. A novel technique for the productions of conical nanopores through a polymer membrane is also reviewed. Nanochannels and nanopores have many potential applications for drug delivery, immunoprotection of cell implants, blocking of globular proteins from biosensor surfaces, and diagnostic devices. All of these applications benefit from the more direct interactions of devices with biomolecules.

El presente trabajo presenta una revisión literaria sobre los métodos de fabricación de nanocanales en silicio y diferentes materiales poliméricos; y su uso en control de transporte molecular. Se describe el método "Sacrificial Layer Lithography" para silicio y polímeros. Adicionalmente, una novedosa técnica para la producción de nanoporos cónicos a través de una membrana polimérica es descrita. Los nanocanales y los nanoporos poseen diversas aplicaciones potenciales en la liberación de drogas, en la inmunoprotección de implantes celulares, el bloqueo de proteínas globulares en la superficie de biosensores, y en dispositivos para diagnóstico. Todas estas aplicaciones se benefician de la interacción directa entre los dispositivos y las biomoléculas.

Lipossomas e suas aplicações terapêuticas: estado da arte / Liposomes and their therapeutic: state of art applications

Lipossomas são vesículas constituídas de uma ou mais bicamadas fosfolipídicas orientadas concentricamente em torno de um compartimento aquoso e servem como carreadores de fármacos, biomoléculas ou agentes de diagnóstico. A estabilidade dos lipossomas pode ser afetada por fatores químicos, físicos e biológicos. Após administração intravenosa, lipossomas convencionais são rapidamente capturados pelo sistema fagocitário mononuclear. Para evitar essa captura, lipossomas furtivos foram desenvolvidos, os quais apresentam a superfície modificada com componentes hidrofílicos. Para permitir a liberação seletiva do fármaco nos sítios alvos, ligantes de reconhecimento específico são conjugados na superfície de lipossomas. Em geral, os métodos de preparação de lipossomas incluem hidratação de um filme lipídico seguida de sonicação ou extrusão para redução do tamanho das vesículas. Os lipossomas são caracterizados quanto ao tamanho e composição química das vesículas e conteúdo do material encapsulado. Nesta revisão, constata-se que os lipossomas oferecem maior eficácia e segurança com relação aos tratamentos convencionais. Apesar de algumas formulações lipossomais serem comercializadas desde os anos 1980 para tratamento de infecções fúngicas sistêmicas e do câncer, problemas de ordem tecnológica e biológica fazem com que os lipossomas sejam ainda extensivamente estudados para desenvolvimento de formulações estáveis no organismo visando à terapia de várias doenças, principalmente do câncer.

Liposomes are vesicles that consist of one or more concentric phospholipidic bilayers organized around an aqueous inner compartment. They are carriers of drugs, biomolecules and diagnostic agents. The stability of liposomes can be influenced by chemical or physical factors. Once injected in the circulatory system, conventional liposomes suffer uptake by the mononuclear phagocytic system. To avoid such a capture, non-conventional or Stealth® liposomes were developed, which are coated by hydrophilic components. Specific ligand had been incorporated in their surface so as to control the drug release and selectivity, thereby originating the targeted liposomes. In general, all preparation methods of liposomes include the hydration of lipidic film followed by sonication or extrusion to reduce the mean size of vesicles. After preparation, the vesicles are characterized by size, chemical constitution, and amount of encapsulated material. In this review it is shown that the liposomes offer effectiveness and safety in comparison with many conventional treatments. In spite of the existence of a few liposomal formulations available since early 80's, which are used for the treatment of systemic fungal infections and cancer, many technological and biological issues remain as a challenge. In this scenario, liposomes have extensively been studied in order to improve their in vivo stability in the treatment of several diseases, including cancer.

Efeitos da suplementação com zinco na redução do estresse oxidativo em indivíduos com diabetes mellitus do tipo 2 / Effects of the zinc supplementation in the oxidative stress reduction with type 2 diabetes mellitus individuals

O zinco é um elemento-traço essencial que está envolvido em diferentes processos metabólicos. Possui função antioxidante, uma vez que faz parte da estrutura da enzima superóxido dismutase (CuZn-SOD), atua na restrição da produção endógena de radicais livres e, na estabilização da estrutura de membranas. Diabéticos geralmente apresentam deficiência de zinco, além de possuírem um grau aumentado de estresse oxidativo, que é gerado pela própria doença. . Portanto o presente estudo teve como objetivo verificar se a suplementação com zinco em pacientes com diabetes mellitus do tipo 2 poderia melhorar as características indicativas de dano oxidativo. O estudo realizado foi duplo cego, sendo administrado zinco aminoquelado (20mg/dia) e placebo, durante 6 meses, sendo os parâmetros do estudo avaliados antes e depois da suplementação, para que fosse possível se observar as mudanças nos parâmetros indicativos do estresse oxidativo. Na avaliação antropométrica, se verificou peso, estatura, medidas da cintura e bioimpedância elétrica. Foi feita a avaliação dos parâmetros glicêmicos e lipídicos, do mineral zinco, bem como dos indicadores de estresse oxidativo, incluindo os óxidos de colesterol e os autoanticorpos anti-LDL eletronegativa. A avaliação alimentar foi realizada por meio do registro de consumo alimentar. Como resultados, podemos ressaltar alta prevalência de sobrepeso/obesidade. Após a suplementação, as concentrações do zinco no plasma e eritrócito tiveram um leve aumento, passando de 68'mais ou menos'10 para 72,9'mais ou menos'13 'mü'/dL, e de 40,7 'mais ou menos'6,2 para 43 'mais ou menos'6,8 'mú'Zn/gHb, respectivamente, entretanto, este aumento não foi estatisticamente significativo. Também não foram encontradas diferenças significativas nas concentrações dos autoanticorpos anti-LDL eletronegativa e do total dos óxidos de colesterol, apesar de existir tendência de melhora destes parâmetros...

Zinc is an essential trace element that is involved with a variety of metabolic processes. It has an antioxidant function as it is part of the superoxidedismutase (CuZn-SOD) enzyme. It works in the restriction of the free-radicals endogenous production and in the membrane structure stabilization. Diabetic people often present zinc deficiency, besides of having an augmented degree of oxidative stress, which is generated by the own disease. Therefore, the present study had as objective to verify if the zinc supplementation would better the indicating characteristics of oxidative damage to type 2 diabetes mellitus patients. The study done was double-blind, with the administration of zinc chelate (20mg/day) and placebo, during 6 months. The study parameters were assessed both before and after the supplementation, in order to be possible for one to observe changes in the indicating oxidative stress parameters. In the antropometric evaluation, it was verified the weight, height, the measure of the waistline and the electric bioimpedance. It was evaluated the glicemic and fat parameters, zinc, as well as the oxidative stress indicators, which included the oxysterols and the electronegative anti-LDL autoantibody. The alimentary evaluation was done by means of the feed consumption registration. As a result, it could be highlighted the predomination of overweigh/obesity. After supplementation, the zinc concentrations in the plasma and erythrocyte showed a slight increase, from 68±10 to 72,9±13mg/dL, and from 40,7±6,2 to 43±6,8mgZn/gHb, respectively. However, this increase was not statically significative. Besides that, it was not found significative differences in the concentration of the electronegative anti-LDL autoantibody and of the oxysterols totals, despite of a trend in improvement of those parameters.

Estudo farmacognóstico, farmacológico e toxicológico de Anacardium occidentale Linn. (Anacardiaceae) clone CCP-76 / Pharmacognostic and toxicological study of Anacardium occidentale (Anacardiaceae) clone CCP76

Os extratos totais, assim como os compostos fenólicos isolados de diferentes partes de Anacardium occidentale conhecido popularmente no Brasil como cajueiro mostraram atividades antiúlcera e antibacteríana. O objetivo deste trabalho foi a verificação destas atividades nas folhas após estudo farmacobotânico, químico e toxicológico. Para a analise anatômica foram utilizados cortes do terço mediano inferior da lâmina foliar. Nesta, as epidermes em vista frontal apresentam cutrcula estriada, na face abaxial, a epiderme é constituída de células de formato poligonal, com paredes bem justapostas. Na face adaxial, as células são de paredes espessas, ligeiramente onduladas. A mesma é constituída de estômatos de tipo anomocítico e de tricomas glandulares de forma ovóide. O mesófilo é constituído de duas camadas de parênquima paliçádico, espessas, de forma quase regular e de parênquima lacunoso com células de forma irregular, envolvendo os feixes vasculares de nervuras secundarias. Extensões de fibras são observadas no mesófilo. A nervura mediana possui um colênquima desenvolvido e ductos são encontrados no floema assim como no parênquima medular. Drusas são encontradas no parênquima lacunoso assim como no parênquima fundamental e no colênquima. A partir da triagem fitoquímica, da cromatografia em camada delgada, cromatografia liquida de alta eficiência e cromatografia liquida acoplada a espectrometria de massa, verificou-se a presença nas folhas de cajueiro de compostos polifenólicos, particularmente de heterosfdeos flavonóidicos. As estruturas de flavon6ide que parecem ser mais evidentes, de acordo com a cromatografia liquida acoplada a massa, são principalmente os heterosídeos da quercetina...

Crude extracts as well as phenolics isolated from the bark or the fruit of Anacardium occidentale popularly known as cajueiro in Brazil, showed antiulcer and antibacterial effects. The aim of this work was to verify those effects in the leaf, botanical, chemical and toxicological studies. Ultrastructure of the leaf was carried on. Cross-sections from the third inferior part of the leaf blade were used. Cashew leaf contains uniseriate epidermis with a sub-eperdimic layer, anomocytic stomata and glandular ovoid trichomes on the inferior surface. The mesophyll exhibits two cell layers of palisadic parenchyma and a lacunose parenchyma containing vascular bundles of the secondary nervures. The median nervure contains a developed collenchyma. Several druses of calcium oxalate are present in the fundamental parenchyma, lacunose parenchyma and in the collenchyma. Resin ducts are also observed in the phloem as well as in the medullar parenchyma. Extensions of sclerenchymatous fibres are observed in the mesophyll. By phytochemical analyses using TLC, HPLC-DAD and positive ions LC-ESIMS, we verified the presence of polyphenols in cashew leaves particularly heterosids of flavonoids. From LC-ESI-MS, evident structures of flavonoids seemed to be heterosids of quercetin. Ethanol 70% extract of cashew leaves was used for antiulcer and antibacterial essays. With extract dose 400mg/kg, ulcer lesions induced by HCL/ethanol 60% in rats, decreased about 98%. By a dose-response effect study, ED50 was evaluated about 150 mg/kg. Extract doses higher than 100mg/kg showed potential of lesion inhibition superior to lansoprazol 30mg. Extract methanolic fraction that gave 88,20% of ulcer inhibition likely contains the principie active of the antiulcer effect. Using bacterial strains, Staphylococcus aureus ATCC 25923, Escherichia coli ATCC 35218 and ATCC 25922, Pseudomonas aeruginosa ATCC 27853 and a clinical isolate Campylobacter coli, for antibacterial essay, the ethanolic extract and one fraction rich in flavonoids were only active in S. aureus with MIC and MBC equal to 320 µg/mL. Acute, 30-day and 90-day subacute toxicity studies were carried out. Crude extract DL50 was superior to 2000mg/kg. Based on biochemical analyses for the evaluation of renal and hepato-biliary functions, level of urea, creatinine, transaminases, total protein, albumin, bilirubin, cholesterol and calcium proved that the extract is biologically tolerated by rat organismo This result was also confirmed by studies in hematology and histopathology. Genotoxity was accessed by Ames test in Salmonella typhimurium strains TA97, TA98, TA100, TA102 and bone marrow micronucleus test in mice. The extract exhibited sign of frameshift and base pairs substitution. Extract dose 2000mglkg seemed to induce damage in the chromosomes however; the activity was extremely inferior to the c1astogenic effect induced by ciclophosphamide.