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Objective To compare the effects of celecoxib and amniotic membrane suspension (AMS) on corneal neovascularization (CNV) area and expression of matrix metalloproteinase-2 (MMP-2) and matrix metalloprotein-ase-9 (MMP-9) in the growth of corneal neovascularization after thermal burn in rabbits,and provide a theoretical basis of celecoxib for the clinical treatment of corneal neovascularization.Methods Left corneas of 36 rabbits were burned by the home-made burning-device,and randomly divided to three groups:negative control group (n =12),AMS group (n =12) and celecoxib group (n =12),were respectively sub-conjunctival injected by 90 g · L-1 saline (0.1 mL),AMS (0.1 mL) and 8 mg · mL-1 celecoxib solution (0.1 mL).The histological morphology,growth condition and area of CNV were compared under slit lamp microscope at 4 days,7 days and 14 days after thermal-burned.At 7 days after thermal-burned,four appropriate corneas were randomly taken to detect the expression of MMP-2 and MMP-9 by immunohistochemistry,and the results were analyzed by computer image analysis system.Results At 4 days,7 days,14 days after thermal-burned,the areas of neovascularization were (11.32 ± 1.11)mm2,(38.49 ± 4.64) mm2,(43.30 ± 4.39) mm2 in negative control group,(9.69 ± 1.30) mm2,(31.15 ± 4.85)mm2,(37.19 ± 5.27) mm2 in AMS group,(8.47 ± 1.20)mm2,(30.31 ± 4.93) mm2,(36.69 ± 3.54) mm2 in celecoxib group,respectively.At different time points,neovascularization area in AMS group or celecoxib group was significantly lower than negative control group (all P ＜ 0.05).There was no difference between AMS and celecoxib group (all P ＞ 0.05).At 7 days after thermal-burned,the expression of MMP-2 and MMP-9 was not different between AMS group and celecoxib group (all P ＞ 0.05),and significantly lower than negative control group (all P ＜ 0.05).Conclusion Celecoxib and amniotic membrane suspension can all effectively inhibit CNV after thermal-burned,which may be related to the down-regulated expression of MMP-2,MMP-9 in thermal-burned corneas.
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AlM:To explore the suitable conditions in rapid model of corneal neovascularization ( CNV ) after thermal burn under different constant temperature in rabbit. METHODS: Total 45 New Zealand white rabbits were divided randomly into five groups ( A, B, C, D, E ) . A groups:100℃ ( n = 10 ) , B groups: 200℃ ( n = 10 ) , C groups:300℃ ( n=10 ) , D groups: 400℃ ( n=10 ) , and E groups:control group ( n=5 ) . All left eyes of rabbits in A,B,C,D groups were induced corneal neovascularization by constant temperature burning device. The growth of CNV was observed by slit lamp microscope and the area of CNV were recorded on 4 th , 7 th , 14 th , 30 th days postoperatively. SPSS 19. 0 statistical package was used for data analysis, and the data was recorded by mean ± standard deviation. Comparison by analysis of variance was made by repeated measures in the area of neovascularization at each time point in groups. Statistical tests were considered significantly when P values were less than 0. 05. RESULTS:On postoperative 4 th , 7 th , 14 th , 30 th days: no neovascularization was found after corneal thermal burn in A group, but only a few nebula left (n=2);the area of CNV were (9.16±1.45)mm2, (37.73±5.49)mm2, (62.44± 7. 54 ) mm2 , ( 40. 28 ± 7. 39 ) mm2 in B group respectively;and (11.45±1.04)mm2, (44.51±4.64)mm2, (66.13±4.13)mm2, (43.04±2.33)mm2 in C group respectively; and (13.23± 0.86)mm2,(47.26±4.59)mm2,(67.57±4.56)mm2,(45.59± 4. 44 ) mm2 in D group respectively, and part corneal carbide ( n = 4 ) was observed as well as corneal perforation ( n= 6 ) were found on 3d in D group. No neovascularization was found in normal control group. Comparison of the areas of CNV at each time point between groups was statistically different, P COCLUSlON:ln 4 to 7d, the higher the temperature is, the more the neovascularization area of CNV are. lt has no significant difference in 14 to 30d. But corneal carbide and corneal perforation are often found in 400℃ group, so its modeling failure rate is high. lt is between 200℃ and 300℃ that repeatability and uniformity of the corneal neovascularization model of rabbit are superior.
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PURPOSE: In order to rehabilitate patients with replanted fingers or reconstructed ones with free flap, the warm therapy is cost-effective, clean, and easy to use. However, the risk of thermal burn in patients with replanted fingers or reconstructed ones with free flap is not clearly identified yet and only few clinical prognosis exist. For that reason, this study was designed to evaluate the incidence, characteristics and risk factors of thermal burn in patients with replanted fingers or reconstructed ones with free flap. METHODS: We reviewed all the medical records of patients retrospectively, especially who showed clinically important thermal burn of replanted fingers or reconstructed ones with free flap from February 2010 to March 2011. RESULTS: 10 patients out of 370 with successfully replanted fingers or reconstructed ones with free flap presented clinically important thermal burn. (2.70% incidence). The causes of thermal burn were warm therapy (4 cases) and touching hot bowls such as a coffee cup, hot pot or hot grill (6 cases). Among them, 7 patients suffered superficial 2nd degree burn with bullae, 1 patient deep 2nd degree burn, 1 patient 4th degree burn and another 1, 1st degree burn with mild erythema. All of them except two cases were treated with conventional dressing with antibiotic ointment, while one was treated by skin graft and another one by 2nd toe pulp free flap. On the other hand, there were only 3 thermal burn cases among 7,010 patients who had undergone the hand surgeries other than replantation and free flap (0.04% incidence). And 2 were by warm therapy and 1 by hot pot. All of them were superficial 2nd degree burn with bullae and treated with conventional dressing with antibiotic ointment for about 1 week. CONCLUSION: These results suggest that patients with replanted fingers or reconstructed ones with free flap are more likely to have thermal burn than any other. Therefore we should be aware of the possibility of thermal burn for these patients, paying more attention to prevent it during the warm therapy and letting them always keep an appropriate distance from anything that can cause thermal burn.
Subject(s)
Humans , Bandages , Blister , Burns , Coffee , Erythema , Fingers , Free Tissue Flaps , Hand , Incidence , Linear Energy Transfer , Medical Records , Prognosis , Replantation , Retrospective Studies , Risk Factors , Skin , Toes , TransplantsABSTRACT
Although a basal cell carcinoma usually results from chronic irradiation of solar ultraviolet, some reports have mentioned that non-solar ultraviolet and trauma also could be etiologic factors of basal cell carcinoma. Herein, we report a case of a 64-year-old man who had worked as a welder for more than thirty years. Four years ago, he was burnt on his right upper eyelid during welding. After that, the lesion had been darkened and indurated, and finally was diagnosed as basal cell carcinoma. The significance of non-solar ultraviolet irradiation and thermal burn during welding in the pathogenesis of basal cell carcinoma is discussed.