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Objective To observe the effects of liposome-adriamycin (L-ADM) and thermotherapy on proliferation and apoptosis of SWO-38 glioma cells. Methods The SWO-38 glioma cells were cultivated in vitro. The effects of thermotherapy (43X1), ADM chemotherapy, L-ADM chemotherapy, thermotherapy + ADM chemotherapy, and thermotherapy + L-ADM chemotherapy on the cell proliferation and apoptosis were observed. The working concentration of ADM and L-ADM, and the cell proliferation rate were determined by MTT method. The apoptotic rate was determined by flow cytometry. Results The proliferation inhibition rate of thermotherapy + L-ADM chemotherapy and thermotherapy + ADM chemotherapy was 80.16% ± 3.78% and 62.09% ± 3.05%, respectively, and it was significantly higher than that of L-ADM chemotherapy (40.27% ± 2.32%), ADM chemotherapy (30.56% ± 2.03%) or thermotherapy (16.51% ± 1.26%, P<0.05), and the proliferation inhibition rate of thermotherapy + L-ADM chemotherapy was higher than that of thermotherapy + ADM chemotherapy (P<0.05). The apoptotic rate of thermotherapy + L-ADM chemotherapy and thermotherapy + ADM chemotherapy was 84.19% ± 2.69% and 60.29% ± 1.47%, respectively, and it was significantly higher than that of L-ADM chemotherapy (46.72% ± 2.09%), ADM chemotherapy (35.09% ± 1.46%) and thermotherapy (17.85% ± 0.78%, P<0.05), and the apoptotic rate of thermotherapy + L-ADM chemotherapy was higher than that of thermotherapy + ADM chemotherapy (P<0.05). Conclusion Thermotherapy can enhance proliferation inhibition and apoptosis induction effect of ADM and L-ADM on SWO-38 glioma cells, and this effect is even stronger with L-ADM.
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Objective To evaluate the effectiveness and safety of interventional thermochemotherapy for VX2 transplanted hepatic tumor model in experimental rabbits.Methods The hepatic tumor model was established in 20 New Zealand rabbits by implanting VX2 tumor cells into the right hepatic lobes of the rabbits.The rabbits were randomly and equally divided into two groups with 10 rabbits in each group:group A (study group)and group B(control group).Via the femoral access,the catheter was placed into the tumorfeeding artery,which was confirmed by DSA.The rabbits in group A received an infusion of 100 ml 5% glucose plus 5-Fu(20 mg/kg)at 60℃ temperature,while the rabbits in group B received an infusion of 100 ml 5% glucose plus 5-Fu(20 mg/kg)at normal room temperature(22-25℃).Before and after the procedure,the tumor size was measured by means of B ultrasonography and the serum ALT was estimated to assess the liver function.The results were statistically compared between two groups.Results After the treatment,the tumor size in group A(study group)became much smaller than that in group B(control group),with P＜0.05.And no significant difference in the serum ALT level existed between group A and group B.Conclusion The interventional thermochemotherapy is superior to interventional chemotherapy in inhibiting VX2 transplanted hepatic tumor in experimental rabbits.
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Objective Effectiveness of two kinds of thermochemotherapy infusion from intraarterial approach were studied in the grafted liver VX2 tumors of rabbit.Methods VX2 tumor model was established in 30 Newzland rabbit's livers.Percutaneous transfemoral hepatic arterial catheterization with fixation of the cathether tip inside the feeding vessel was carried out under DSA guidance.All 30 rabbits were divided into three groups(n=10 in each group),normal temperature 100 ml saline+Adriamycin(ADM)infusion(group 1),60℃100 ml saline+ADM continuous perfusion(group 2)and 60℃100 ml saline+ADM intermittent perfusion(group 3).After the perfusion,the lasting time periods of 43-45℃for tumor tissue of group 2 and 3 together with the concentrations of ADM within tumor's tissue were measured.Results Concentrations of ADM were shown as(12.013?2.237)?g/ml,(17.622?1.368)?g/ml,and(11.519?1.225)?g/ml for group 2, group 3 and group 1 respectively.60℃intermittent perfusion vs 60℃continuous perfusion showed P＜0.05, 60℃continuous pefusion vs normal temperature perfusion also showed P＞0.05. 43-45℃period lasting time (min)for 60℃continuous pefusion vs 60℃intermittent pefusion were(4.1?2.7)min and(11.3?3.3)min respectively,the latter was three times more than the former.There were no differences shown betwen the temperature,respiration and heart rate of group 2 and group 3.Conclusion Intermittent intraarterial perfusion thermochemotherapy is a more effective interventional management among all thermochemotherapies.
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Objective To explore the pathological changes by CT scan on localized thermochemotherapy dur- ing and after the operation of human gliomas.Methods Retrospective analysis was given to the CT scan of 37 pa- tients receiving thermochemotherapy during and after the operation,and the relation of the tumorous cells and mi- crovessels and CT density by EM were analyzed.Changes of tumorous cells and microvessels after localized ther- mochemotherapy on C_6 gliomas in rat were analyzed.Results When the tumor was low dense on CT pattern,less cellular number with increasing the amount of fluid between the cells was demonstrated pathologically.On EM,a lower cellular electron density was observed.The amount of fluid in cytoplasm was increased,the cytoplasm was porous,swelling denaturation was chiefly seen in organelle.If the tumor had mixed density on CT,cellular number was more,the amount of fluid was less.On EM,cellular electron density increased correspondingly,the fluid in cyto- plasm decreased,organdie was aggregated.After thermochemotherapy,the tumor reduced,liquefied,and vanished by CT scan.It could be observed that the tumorous cell become smaller,concentrated and cataclased,finally formed apoprotic bodies and separated from the cell in C_6 gliomas in EM.The tumorous vessels was less,smaller and thinker. Some vessels only could see the base membrane and no endothelioid cells.Conclusion The remaining tumors is van- ished by CT scan.The mechanisms of tumors disappearance proposes to explain that thermochemotherapy can dam- age C_6 glioma cells and microvessels,decrease microvessels density and induce tumor ceils apoptosis.That inhibits tu- morous angiogenesis and proliferation.
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Objective To evaluate the clinical efficacy of transcatheter hepatic arterial thermo-chemotherapy and thermo-lipiodol embolization in the treatment of hepatic metastases from colorectal carcinoma.Methods Sixty-eight cases with hepatic metastases from colorectal carcinoma were equally and randomly divided into two groups.The patients in study group were treated with transcatheter hepatic arterial thermo-chemotherapy and thermo-lipiodol embolization,while the patients in control group were treated with conventional(normal temperature) transcatheter hepatic arterial chemotherapy lipiodol embolization.Results The effective rate of study group and control group was 65%(22 / 34) and 32%(11 / 34) respectively,the difference between two groups was statistically significant(P
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Objective:To observe the recent effects and toxicity of thermochemotherapy on malignant hydrothorax or hydroperitoneum,to evaluate the changes of the immunological functions,and to investigate the mechanism of thermochemotherapy.Methods:Fifty-two patients were treated with weekly intracavitary chemotherapy,and then combined with local endogenetic thermotherapy twice a week.As the control,another 50 patients received weekly intracavitary chemotherapy.The treatment lasted for two weeks and was followed by one-week rest,and then the recent effects and toxicity were observed.The T cell subset,NK cells and VEGF levels in serum,hydrothorax or hydroperitoneum were tested.Results:Overall response rates of the malignant hydrothorax were 86.9% vs 60.0%(P
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Objective To study the effects of localized thermochemotherapy on angiogenesis and the expression of VEGF protein of C6 gliomas in rats,and explore the mechanisms of angiogenesis inhibition by localized thermochemotherapy. Methods Following the establishment of animal models, 40 rats harboring the C6 gliomas were randomly allocated to 4 groups,the rats in the fore 3 groups were treated by localized thermochemotherapy, hyperthermia and chemotherapy, respectively, the remaining group served as control. The expression of VEGF and FⅧ RA protein were detected by S P immunohistochemistry. The vascular structure in rat's glioma was observed by immunohistochemistry and electron microscopy technique. Results As compared with the control group, the expression of VEGF protein was decreased in the hyperthermia and chemotherapy groups,as well as in the thermochemotherapy group.The expression of VEGF was positively correlated with the microvessel density in the tumor ( r =0.9798, P
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Objective To study the effects of localized thermochemotherapy on cellular proliferation and the expression of PCNA and IGF-I protein of C6 gliomas in rat, and explore the possibility of developing new therapeutic method of gliomas. Methods C6 glioma cells were injectcd subcutaneously to rats. The rats were randomly assigned to 4 groups,and treatment were initiated on day 22 after tumor inoculation. Prior to treatment, the subcutaneous gliomas were examined to verify tumor size, which were ranged 1.5 to 2.0 cm. Then localized thermochemotherapy, hyperthermia and chemotherapy were given, respectively. The size and weight of subcutaneous tumors were measured. PCNA and IGF-I protein expression were detected by the technique of S-P immunohistochemistry. Glioma cells proliferation was detected by HE staining method and electron-microscopic observation. Results Compared with the control group, the tumor size of rats in the hyperthermia and chemotherapy as well as thermochemotherapy groups was decreased,the tumor weight was also decreased significantly (2.95g vs 1.95g vs 1.86g vs 1.09g, P
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AIM: To investigate the effect of apoptosis and the change of mitochondial tansmembrane potential in A549 cells induced by thermochemotherapy of ADM. METHODS: ADM of different concentrations was directly applied to A549 cells cultivated in vitro. Cells maintained at 42.5 ℃ for 30 min. RESULTS: The inhibition of A549 cells was increased by thermochemotherapy of ADM significantly,and the cytoplasmic concentration of thermochemotherapy of ADM was significantly higher than that of chemotherapy (P
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Objective To evaluate the clinical efficacy of thermo-chemotherapy and thermo-lipiodol embolization intreatment of primary hepatocellular carcinoma(PHC).Methods One hundred and sixteen cases of PHC were divided into three groups.Group A(38 cases)was treated with normal temperature chemotherapy and normal temperature lipiodol,Group B(40 cases)with thermo-chemotherapy and normal temperature lipiodol and group C(38 cases)with thermo-chemotherapy and thermo-lipiodol.Group B and group C were called the thermotherapy group.Results In the thermotherapy groups,the rates of tumor size reduction were significantly greater than those in the normal group.There were no significant different in the hepatic function tests among the three groups.The 6-,12-,18-,and 24-month survival rates of the normal group and thermotherapy groups were 97%,58%,39% and 18%,versus 99%,79%,57% and 36%,respectively.No significant differences were found in the rates of reduction of tumor size and survival rates between group B and group C.Conclusion Thermo-chemotherapy and thermo-embolization possess significant effect on PHC but without conspicuous damage to liver function.
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Objective The research is to study the temperature rising situation in the thermochemotherapy process of the domestic dog through the hepatic artery, and the bearing ability of heart, liver, and kidney during the process, as well as the biochemical and histological change of the liver after the procedure. Methods A thermostated container of water bath and an infusion pump, both designed domestically, were adopted. Ten domestic dogs were divided into 2 groups of A and B with 5 dogs respectively. Through hepatic artery infusion for group A, glucose solution (GS) was administered at 43?C for 30 minutes, with the flowing rate of 30ml/min, in group B 5% GS at 45?C for 30 minutes, with the flowing rate of 40ml/min added with chemotherapy drug MMC(0.2mg/kg) was administered to 3 dogs and 5% GS with the same flowing rate added with CDDP(1mg/kg) into other 2 dogs. Results The temperature of anus and liver was 0.3～0.5?C and 0.5～1.0?C higher than before the procedure respectively, there was no significant rate changes of heart and respiration during and before the procedure. Four dogs were radiographied after 15 minutes' infusion, showing different degrees of hepatic artery dilation accompanied with relevant increase of infusion. Six dogs were radiographied after 30 minutes' infusion, 5 of them showed arterial contraction astescal on accompanied with relevant decrease of infusion. There appeared slight or moderate increase of aminopherase after the procedure, but in actually returned to normal within a week. Pathologically, there appeared no acute effect on heart, lung and kidney other than slight and reversible damage to hepatic tissue. Conclusions Healthy dogs can endure the chemotherapy treatment of infusion through hepatic arterial fistula at 43?C 45?C for 30 minutes.
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Objective To investigate the target effect of cytosine deaminase genetherapy combined thermochemotherapy on liver metastasis of colon cancer in nude mice.Methods Plasmid G1CEACDNa were transferred into the human colon cancer LoVo cells by liposomes method.The models of liver metastasis of colon cancer were established by injected LoVo-CEACD into peritonum in 45 BALB/C nude mice,then which were randomly divided into three groups:The control(C)group;the prodrug therapy(PDT)group and the prodrug thermochemotherapy(PDTCT group)and 42 ℃ sodium chloride,38 ℃ 5-FC and 42 ℃ 5-FC were injected into the abdominal cavity [500 mg/(kg?d),for 21 d],respectively.After natural death of all mice,their life span,rate of liver metastasis and number of liver metastasis nodules were surveyed and compared among the three groups.The levels of the expression of target gene were detected by RT-PCR in carcinoma tissue,normal liver,stomach,lung,pancreas,small intestine and large intestine tissues.Pathological features were observed.Apoptotic index(AI)of tumor cells were analyzed in every group respectively.Results The CD gene was detected in the tumor tissues,but not in tissues of normal liver,stomach,lung,pancreas,small intestine and large intestine.In C,PDT and PDTCT group,the life span was(25.80?3.65),(34.27?4.08)and(41.87?3.91)d respectively;the rate of liver metastasis was 100.0%(15/15),40.0%(6/15)and 13.3%(2/15),respectively;the number of liver metastasis nodules was(2.93?1.16),(0.80?1.01)and(0.20?0.56),respectively;and AI of tumor cells was 4.59%,9.87%,17.4%,respectively.The life span of PDT and PDTCT guoup was significantly longer than that of group(P
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Objective To investigate the effect of thermochemotherapy with 5 fluorocytosine(5 FC) on human colorectal carcinoma cell lines SW480 transfected with carcinoembryonic antigen (CEA) tissue specific cytosine deaminase (CD) in vitro.Methods Recombinant retroviral vector G1CEACDNa, in which CD gene was controlled under the CEA promoter, was introduced through liposome technique to the human colorectal carcinoma cell line SW480, and then the cells were selectively cultured in G418. The proliferated colonies were treated with the combined therapy of 5 FC and hyperthermia at a 43℃ over 30 min for 3 times. RT PCR was performed to analyze the expression of CD gene in target cells after thermochemotherapy. Results The CD gene expressed steadily in the target cells after 3 times of hyperthermic treatment. After the transfection of CD gene, SW480 CEACD cells were more sensitive to 5 FC than their parental cells (P
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Objective To develop a whole-automatic intellectual thermochemotherapy equipment of peritoneal perfusion by which hyperthemic chemotherapeutics were post in peritoneal cavity with constant flow pump.Methods MSP430 one chip whole-automatic machine was adopted to monitor and keep chemotherapeutic temperature.Results Being treated with peritoneal perfusion thermochemotherapy after radical excision,one-year and three years survival rates of the patients with advanced gastric carcinoma were increased 8.12% and 20%,meso-life span extends 18 months.Conclusion The thermochemotherapy equipment of peritoneal perfusion can eliminate dissociating malignant cell in peritoneal cavity and minimum cancer focus on peritoneal membrane,and prevent peritoneal recurrence effectually.