ABSTRACT
Objective:To explore the mechanism of the treatment of diabetic feet with thunberg fritillary and honeysuckle based on pharmacological and animal experiments.Methods:The drug ingredients of the drug pair of thunberg fritillary and honeysuckle were obtained from TCMSP, and the targets of the drug pair were obtained from the Swiss target prediction. The targets of the disease target of diabetic foot were screened. The cross target was obtained using Venny 2.1. A protein-protein interaction(PPI) network was constructed by STRING and Cytoscape software. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses were carried out by Metascape. The ingredient-target-pathway network was established by Cytoscape software. The diabetic foot rat model was established, and the effects of the treatment and the detection of serum screening targets were observed.Results:The results show that 38 active ingredients in the drug pair acted on 339 disease targets to treat diabetic feet through multiple pathways. The core ingredients include luteolin, pelargonidinin, ziebeimine, peiminine, adenosine, and TP53, EP300, HSP90AA1, CTNNB1, and Akt1 are the critical targets. The results of the genetic function analysis show that the biological process is mainly involved in the combination of cellular transcription factors, the reaction of cells to nitrogen compounds, and hormone reactions. The main components of the cell component mainly involve the film raft, the membrane raft, the small space and so on. The molecular function mainly involves protein kinase activity, phosphate kinase activity, kinase activity and so on. The results of the KEGG enrichment analysis indicate the signaling pathways mainly include PI3K/AKT, HIF-1, EGFR, and MAPK. The local repair effect of the drug pair on the diabetic foot rat model is significant, and the expression of the serum TP53, EP300, HSP90AA1, CTNNB1, Akt1 is significantly increased.Conclusions:The mechanism of the treatment of diabetes foot with thunberg fritillary and honeysuckle is mainly by regulating targets (TP53, EP300, HSP90AA1, CTNNB1 and Akt1), signal pathways (PI3K/AKT, HIF-1, EGFR and MAPK), and biological processes such as enzymatic activity and anti-inflammatory.