ABSTRACT
Aim: To study the protective effect of five different compounds from Edgeworthi gardneri Meissn on carbon tetrachloride (CCl4)-induced liver injury in mice and the possible mechanisms. Methods: The models of acute liver injury were induced by CCl4 in rats, which were then randomized into blank group, model group, positive control group, porphyrin low and high dose group (ZL, ZH), daphnoretin low and high dose group (XL, XH), vanillin C low and high dose group (JL, JH), ortho-hydroxybenzoic acid low and high dose group (SL, SH), and silver forging glycoside low and high dose group (YL, YH). The serum activities of alanine aminotransferase (ALT), aspartate aminotransferase (AST), contents of MDA and SOD in liver tissue homogenate were determined. The relative mRNA expression of TNF-α, IL-6 in the GAPDH were detected by RT-PCR, and the pathological section of liver tissue of mice was observed. The protective effect and possible mechanism of the five compounds were studied. Results: Compared with model group, ZH, SH, JL, JH, XH, YH groups had obviously low serum ALT and AST levels (P < 0. 05, P < 0. 01). Phillygenin effectively improved the tissue lesion of liver, and each drug group could significantly reduce MDA content and improve SOD content. The relative mRNA expression of TNF-α, except ZL, the other dosage groups had significantly inhibitory effects. Except YL, ZL, XL, there was statistically significant difference in the relative mRNA expression of IL-6 (P < 0. 05, P < 0.01). Conclusions: ZH, SH, JL, JH, XH, YH dosage groups have protective effects on acute hepatic injury induced by CCl4 in mice. The mechanism may involve regulating the immune function of mice, enhancing the antioxidant capacity of liver and reducing the lipid peroxides.
ABSTRACT
Abstract Lepechinia mutica (Benth.) Epling, Lamiaceae, and Vallea stipularis L.f., Elaeocarpaceae, are the object of the present study. These plants are endemic to the Andean region and have attracted our attention on the basis of interesting results obtained in a preliminary anticholinesterase screening. Actually, carnosol and tiliroside, isolated from L. mutica and V. stipularis, respectively, have shown a promising selective inhibitory activity against butyrylcholinesterase. Specifically, the anti-butyrylcholinesterase activity of carnosol was 5.15 µM and that of tiliroside was 52.9 µM, compared to 8.568 ± 0.570 µM of the positive control Donepezil. Carnosol and tiliroside were purified chromatographically from the ethyl acetate extract of L. mutica and V. stipularis, respectively. Spectrophotometric methods were used for enzymatic studies.
ABSTRACT
Extraction and fractionation of Pulsatilla koreana flowers followed by, repeated open column chromatography for EtOAc and n-BuOH fractions yielded four flavonoid glycosides, namely, astragalin (1), tiliroside (2), buddlenoide A (3), and apigenin-7-O-(3"-E-p-coumaroyl)-glucopyranoside (4). The chemical structures of these flavonoid glycosides were elucidated on the basis of various spectroscopic methods including electronic ionization mass spectrometry (EI-MS), 1D NMR (1H, 13C, DEPT), 2D NMR (gCOSY, gHSQC, gHMBC), and infrared (IR) spectrometry. This study represents the first report of the isolation of the flavonoid glycosides from the flowers of P. koreana.
Subject(s)
Chromatography , Flowers , Glycosides , Magnetic Resonance Spectroscopy , Mass Spectrometry , Pulsatilla , Spectrum AnalysisABSTRACT
Objective: To study the chemical constituents from the inflorescences of Tilia amurensis. Methods: The chemical constituents were separated and purified by silica gel, Sephadex LH-20, ODS column chromatographies, and preparative HPLC. Their structures were determined by physicochemical properties and spectral data. Results: Fourteen compounds were isolated from the ethyl acetate fraction of 70% ethanol extract in the inflorescences of T. amurensis, and identified as linarin (1), soquercitrin (2), quercetin (3), astragalin (4), tiliroside (5), 5, 7, 4'-trihydroxy-3'-methoxy isoflavone (6), orobol (7), quercetin-3-O-α-L- arabinopyranoside (8), friedelin (9), β-sitosterol (10), protocatechuic acid (11), theophylline (12), caffeine (13), and palm alcohol (14). Conclusion: Compounds 1-5, 7, 8, and 10-12 are isolated from this plant for the first time, and compound 7 is found in the plants of Tilia Linn. for the first time.
ABSTRACT
Objective: To research the separation, extraction, and quality control of tiliroside from Loropetalum chinense. Methods: The herbs were extracted using ethanol reflux extraction, and then through macroporous resin, depressure silica gel column chromatography, and elution, tiliroside monomer was obtained. Content was determined by the C18 column, mixture of methanol-0.1% phosphoric acid (55:45) as mobile phase at 1.0 mL/min, and UV detection wavelength at 320 nm were used for the determination. Results: Tiliroside can be a good separation of the monomer, with the content of 98%; Tiliroside was better in linear relationship in the range of 0.041-0.513 μg (r = 0.9997). The recovery rate was 100.05%, RSD = 1.50%. Conclusion: Using this method, tiliroside can be better extracted and separated, Meanwhile the determination method is very stable and accurate, and has good repeatability, which can be used for the quality control of tiliroside from L. chinense.
ABSTRACT
Tilia species, among which is Tilia cordata Mill. (Tiliaceae), have been used in folk medicine as anxiolytic. The hydroethanolic extract was analyzed by using liquid chromatography with mass spectrometry HPLC-DAD-ESI-MS/MS in negative ion mode, and its chemical composition was compared to flavonoids reported as anxiolytics. The major flavonoids found were: quercetin-3,7-di-O-rhamnoside, kaempferol-3,7-di-O-rhamnoside and kaempferol 3-O-(6"-p-coumaroyl glucoside) or tiliroside. The anxiolytic activity of the genus Tilia has been attributed to the presence of quercetin and kaempferol derivatives, while the anxiolytic activity of T. americana var. Mexicana was attributed to tiliroside, which was also found among the major constituents of this species.
As espécies de Tilia, entre elas, a Tilia cordata Mill. (Tiliaceae) são utilizadas como ansiolíticas na medicina popular. O extrato hidroalcoólico foi analisado usando cromatografia líquida acoplada à espectrometria de massas HPLC-DAD-ESI/MS/MS no modo negativo e a sua composição química foi comparada com os flavonóides já reportados como ansiolíticos. Os principais flavonóides encontrados foram: quercetina-3,7-di-O-rhamnosideo, canferol-3,7-di-O-rhamnosideo, e canferol 3-O-(6"-p-cumaroil glucosideo) ou tilirosideo. A atividade ansiolítica do gênero Tília tem sido atribuída à presença de derivados de canferol e quercetina, enquanto que a atividade ansiolítica da T. americana var. Mexicana foi atribuída ao tilirosideo, o qual também foi encontrado entre os principais constituintes desta espécie.
Subject(s)
Anti-Anxiety Agents , Tilia europaea/analysis , Quercetin , Glycosides , Gas Chromatography-Mass Spectrometry/methodsABSTRACT
O gênero Croton possui seiscentas espécies e é originário de regiões tropicais. Croton gnaphelil é conhecida no sul do Brasil como infalivina, sendo utilizada popularmente nos distúrbios hepáticos. A substância majoritária presente nas partes aéreas desta planta foi isolada e identificada através de métodos espectroscópicos, sendo identificada como um glicosídeo acilado, o canferol‑3‑O‑(6"‑O‑p‑cumaroil)‑β‑D‑glicopyranose, também chamado de tilirosídeo.
Croton genus comprises six hundred species being native in tropical forests. Croton gnaphalliis popularly known as "infalivina" in South of Brazil and it is traditionally used for hepatic disorders. The major compound from aerial parts of this plant was isolated and its structure elucidated through spectroscopic analysis. It was identified as an acylglycoside, kaempferol‑3‑O‑(6"‑O‑p‑coumaroyl)‑ β ‑D‑glucopyranoside, known as tiliroside.