Management and practice of intra-laboratory Turn-Around-Time in emergency testing laboratory / 国际检验医学杂志

Objective To master the actual work efficiency situation of emergency inspection,analyze the existing problems,and provide objective basis for continuous improvement through the statistic analysis of intra-laboratory turn-around time (TAT) median,coincidence rate of inspection report within the specified time limit,report timeout reasons and other indexes of different items of emergency inspection group in 2015.Methods All kinds of original data from the different items of emergency inspection groupswere derived from LIS system,according to the different types of projects in different segments.Microsoft Excel2003 software was used to count the actual TAT time of various items,and function was used to calculate TAT median;The non-conformance rate (inspection report timeout rate) and coincidence rate within the prescribed time limit by inspection report were calculated,and gather the statistical analysis on the timeout reasons of daily recorded report.Results With the exception of electrochemical luminescence class,the TAT of other items decreased in different degrees of emergency inspection group in 2015 when compared with 2014.The average coincidence rate of inspection report of various items within the time limit was 94.8%,non-conformance rate of inspection report of prescribed time limit was 5.2%.The top four inspection timeout reasons of various items were more number of submitted samples(59.6%),redo the reinspection (12.4%),smear reinspection (12.0%) and equipment failure (10.0%) in turn.Conclusion The time limit of the emergency report meets the specified requirements and statistical analysis of the causes of overtime can find out the problems and take appropriate measures to provide an objective basis for continuous improvement.

Correlation analysis of uterine segment thickness and time limits for patients with prior cesarean section / 局解手术学杂志

Objective To study the correlation of uterine segment thickness and time limits for patients with prior cesarean section. Methods A total of 80 women with the first pregnancy after cesarean section in our hospital from April 2013 to February 2015,according to the interval of cesarean section and second pregnancy,were divided into group A (18 cases,interval ≤3 years),group B (23 cases,an in-terval of 3 to 6 years),group C (27 cases,an interval of 7 to 9 years),and group D (12 cases,the interval ≥9 years).The uterine segment thickness and the success rate of vaginal trial production were compared.Surgical failures underwent vaginal trial production due to cesarean section were observed.Results The uterine segment thickness values of group A and group B in were respectively (5.93 ±0.34)mm and (5.45 ±0.27)mm,which were significantly higher than group C (3.24 ±0.52)mm and group D (2.83 ±0.56)mm,the difference was statistically significant (P <0.05).The vaginal trial production success rates of group A and group B were respectively 88.89% (16 /18) and 78.26% (18 /23),which were significantly higher than group C 44.44% (12 /27)and group D 33.33% (4 /12)(P <0.05).The dos-age of oxytocin of group A was (35.34 ±4.32)mL,blood loss was (256.32 ±34.21)mL, The oxytocin hormone dosage of group B was (37.09 ±4.52)mL,blood loss was (260.11 ±35.53)mL,which were lower than those in group C and group D (P <0.05).The postpartum hemorrhage rate of group A,group B and group C was respectively 0(0 /2),20.00% (1 /5),33.33% (5 /15)lower than group D 87.50%(7 /8)(P <0.05).Conclusion Maternal uterus at cesarean section within 7 years about the degree of recovery are in good condition,the higher uterine segment thickness,this time period is good for subsequent pregnancy and childbirth.

Chemical constituent change regulation of processed aconite during decoction and discussion on time limit of decoction based on metabolomics / 中草药

Objective: To analyze the change regulation of chemical constituent groups in processed aconite during decocting, and also to discuss the scientificity connotation of decoction of processed aconite. Methods: An ultra-high performance liquid chromatography coupled with time of flight mass spectrometry (UHPLC-Q-TOF/MS) was carried out to acquire the chemical constituents information in samples with different decoction time. High precise mass data were processed by multivariate statistical analysis techniques to discover and identify the constituents with significant difference. Furthermore, the content change, toxicity, and activity of these constituents were also discussed. Results: Fifteen chemical markers with significant difference were screened by partial least squares discriminant analysis (PLS-DA) and t test. By analyzing the change regulation of the different constituents during 4 h decocting process, we found that it mainly happened in the constituent dissolution and chemical transformation from monoester alkaloids to aconine alkaloids, which indicates the attenuation process is secondary. Conclusion: In terms of analgesic and anti-inflammatory effect, it suggests that 0.5 h of decoction would be fine, while in terms of cardiotonic effect, it needs the further systemic comparison for cardiac activity between monoester alkaloids and aconine alkaloids to be able to investigate the necessary and scientificity for long time decoction of the processed aconite.

Effect of Priming Technique Combined with Time Limit on Shortening the Onset time of Cisatracurium Be-silate / 中国药师

Objective:To study the effect of priming technique combined with time limit on shortening the onset time of cisatra-curium besilate. Methods:Totally 112 adult patients undergoing selective operation with general anesthesia were randomly divided into group A, B and C. Fentanyl with the dose of 4 μg·kg-1 and propofol with the dose of 2 mg·kg-1 were used in the three groups as the inducing agents. Group A was first with intravenous injection of cisatracurium besilate 20 μg·kg-1 , and 3 minutes later, the in-ducing agents and cisatracurium besilate with the dose of 80 μg·kg-1 were respectively injected. Group B was first with intravenous injection of cisatracurium besilate with the dose of l00 μg·kg-1 , and 1. 5 minutes later, the inducing agents were injected. Group C was respectively injected with the inducing drugs and cisatracurium besilate with the dose of l00 μg·kg-1 . The T1 percentage before the induction, the time of 90% and 100% inhibition of muscle twitching, the rating of trachea cannula and adverse drug reactions in the three groups were observed and compared. Results:There was no significant difference in the excellent rate of trachea cannula a-mong the three groups (P>0. 05). Compared with group A and B, group C showed no T1 suppression before the induction, while group B with T1 of (9. 8 ± 2. 6) was higher than group A with T1 of (3. 2 ± 1. 5) (P<0. 01). The time of 90% and 100% inhibition of muscle twitching in group C was the longest (P<0. 01), and the incidence of adverse drug reactions in group C was also the highest (P<0. 05). Conclusion:The application of priming principle and time limit can effectively shorten the onset time of cisatracurium be-silate with significant effect and high safety.

Preparation and dissolution of Puerariae Radix Flavones Dropping Pills / 中草药

Objective: To prepare Puerariae Radix Flavones Dropping Pills and to investigate the dissolution. Methods: Exterior quality, weight variation, and resolving time were used as comprehendsive evaluation indicators to select dropping conditions by orthogonal design. HPLC was used to determine the content of puerarin and rotating basket method was used to determine the dissolution rate in vitro of the dropping pills and tablets. Results: The optimal preparation conditions for the pills were as follows: proportion of drug and matrix was 1:3, drop rate was 20 d/min, the temperature of drug fluids was 80°C, the condensate tube outlet temperature was 50°C, dimethylsilicone was the refrigerant at the temperature of 10°C and 6 cm distance above liquid level. The content of peurarin in the dropping pills was 5.542 mg/g. The accumulated dissolution rate of puerarin in the dropping pills reached 98.81% in 20 min, while the accumulated dissolution rate of puerarin in tablets was only 10.70% in 20 min. Conclusion: The preparation process and HPLC determination method are simple, stable, and feasible. The dissolution rate of puerarin can be improved in the Dropping pills.

Relationship of the fragmented QRS and the fragmented QRS time limit with ventricular arrhythmia in old myocardial infarction / 中国医师进修杂志

ObjectiveTo explore the relationship of the fragmented QRS (fQRS) and the fQRS time limit with ventricular arrhythmia in old myocardial infarction (OMI) patients through contrasting the incidence of ventricular arrhythmia in OMI patients whether fQRS or not and ventricular arrhythmia in different fQRS time limit.MethodsAccording to the routine electrocardiogram,321 OMI patients were divided into group A (fQRS appearance,167 cases) and group B(fQRS non-appearance,154 cases).The lead with fQRS extense was ehosen and traced another 50 mm/s electrocardiogram,and 3 consecutive fQRS time limit were measured and them average was taken in group A.According to the fQRS time limit,the patients in group A were divided into 3 groups: group X( ≤0.100 s,96 cases),group Y (0.101-0.119 s,54 cases) and group Z( ≥0.120 s,17 cases).All the patients were continuously monitored with 24 hours dynamic electrocardiogram,and the incidence of ventricular arrhythmia was analyzed.ResultsThe incidence of ventricular arrhythmia in group A [ 78.4％ ( 131/167 ) ] was higher than that in group B [ 63.6％(98/154) ] (P＜ 0.01 ).The incidence of premature ventricular contraction(PVC) ＞ 720/24 hours in group A [ 28.7％(48/167 ) ] was higher than that in group B[ 17.5％(27/154) ] (P ＜ 0.05 ).The incidence of multifocal PVC,coupled PVC,nonsustained ventricular tachycardia and Lown 3-5 grades PVC was 16.2％ (27/167),33.5％ (56/167),12.0％ (20/167),34.1％ (57/167) in group A,7.8％ (12/154),21.4％ (33/154),4.5％(7/154),23.4％(36/154) in group B,there were significant differences between two groups (P＜ 0.05 ).The incidence of ventricular arrhythmia in group Z [ 100.0％( 17/17 ) ] was significantly higher than that in group Y [79.6％(43/54)] and group X [74.0％(71/96)](P＜ 0.05).The incidence of Lown 3-5 grades PVC in group Z[ 70.6％( 12/17 )] was significantly higher than that in group Y[ 42.6％(23/54)] and group X [ 22.9％(22/96) ],and the incidence of Lown 3-5 grades PVC in group Y was significantly higher than that in group X (P＜ 0.05).ConclusionsOMI patients with fQRS have higher incidence and severe degree in ventricular arrlhythmia than those without fQRS.With the fQRS time limit widened,PVC and Iown 3-5 grades PVC significantly increased.So fQRS is a new predicting index of OMI,and fQRS time limit has definite value in predicting the heart event for OMI patients.

Reprodutibilidade do tempo limite em mulheres fisicamente ativas / Reproducibility of time limit in women phisically active

A velocidade de VO2max (vVO2max) e o tempo de permanência nesta (Tlim), ultimamente estão sendo utilizadas para a prescrição do treinamento, principalmente o de característica intermitente. Com isso, o presente estudo objetiva verifi car a reprodutibilidade do Tlim em mulheres fi sicamente ativas. Participaram deste estudo 10 voluntárias saudáveis (22,3 ± 2,2anos; 58,04 ± 6,3kg; 166,2 ± 6,56cm; 25,41 ± 2,94%Gord.) que foram submetidos a três testes: 1) determinação da capacidade (LAn) e potência aeróbia (VO2max) através de um protocolo progressivo e intermitente; 2 e 3) Determinação do tempo limite na velocidade associada ao VO2max, Tlim 1 e 2 respectivamente. Em todos os testes foram realizadas coletas de sangue do lóbulo da orelha para a determinação do lactato sanguíneo, e estes foram realizados em esteira rolante motorizada. Para analise dos dados utilizou-se uma estatística paramétrica através do teste “t student” (p≤0,05). Com isso, verifi cou-se que as voluntárias não apresentaram diferenças signifi cantes para o Tlim, FC e [lac] ao fi nal dos testes de Tlim 1 e Tlim 2 respectivamente (239,2 ± 63,82 e 255,5 ± 61,9 seg; 191,2 ± 7,72 e 192,5 ± 9,24 bpm; 8,46 ± 1,40 e 7,71 ± 1,28 mM), as correlações entre o VO2max e a v VO2max (0,77) e o Tlim 1 e o Tlim 2 (0,72) foram positivas e signifi cantes, porém quando correlacionados o Tlim e VO2max (-0,28) e Tlim e vVO2max (-0,02) foram negativas, entretanto não signifi cantes. Portanto, conclui-se que o Tlim foi reprodutível e assim, recomenda-se a sua utilização para avaliação e prescrição de treinamento.

The speed of VO2max (vVO2max) and the time of permanence in this (Tlim), lately are being used for the lapsing of the training, mainly of intermittent characteristic. Thus, the present study objective to verify the reprodutibility of the Tlim in physically active women. Ten (10) healthful volunteers women had participated of this study (22,3 ± 2,2anos; 58,04 ± 6,3kg; 166,2 ± 6,56cm; 25,41 ± 2,94%Gord.) that they had been submitted on three tests: 1) Determination of the capacity (LAn) and aerobic power (VO2max) through a gradual and intermittent protocol; 2 and 3) Determination of the limit time on the speed associated with the VO2max, Tlim 1 and 2 respectively. In all the tests had been carried through collections of blood of the ear lobe for the determination of sanguineous lactate, and these had been done in motorized rolling mat. For the data analyzes was used a parametric statistics through test “t student” (p≤0,05). That way, It had been verifi ed that the voluntary women had not presented signifi cant differences for the Tlim, FC and [lac] in the end of the Tlim 1 e Tlim 2 respectively (239,2 ± 63,82 e 255,5 ± 61,9 seg; 191,2 ± 7,72 e 192,5 ± 9,24 bpm; 8,46 ± 1,40 e 7,71 ± 1,28 mM), the correlations between VO2max and vVO2max (0,77) and Tlim 1 and Tlim 2 (0,72) had been positive and signifi cant, however when Tlim e VO2max (-0,28) and Tlim and vVO2max (-0,02) had been correlated they had been negative, however non signifi cant. Therefore, it had been concluded that the Tlim had been reproductibile, and thus it has been regarded its use for evaluation and lapsing to it of the treatment.

Study on Preparation and Technical Process of Muxiang Shunqi Wan / 中国中医药信息杂志

Objective To investigate the parameter of technical shaping process of Muxiang Shunqi Wan and select appropriate auxiliary materials to enhance the time limit of dissolution. Method Select parameter of technical shaping process by orthogonal test and choose the best dosage of disintegrant by well-proportioned design. Results The best preparation and technical shaping process of Muxiang Shunqi Wan is as follows:the drug powder is refined with CMS-Na 1.4%, CCNa 0.5%, 60 ℃ dry. Conclusion Pills made by this technical process appear round and smooth, the colour and luster are equal and conformable, the dissolving time limit is short and the quality is good, therefore, they are up to the GMP requirements.