ABSTRACT
Objective To explore clinical efficacy and safety of Tongfeng decoction combined with celecoxib for the acute gouty arthritis of damp-heat with blood stasis type.Methods According to the random indicator method,193 of patients with acute gouty arthritis of damp-heat with blood stasis type in our hospital from 2014 January to January 2017 were divided into control group (n=96) and treatment group (n=97).Patients of control group were treated with celecoxib,while treatment group were treated with Tongfeng decoction combined with celecoxib.The two groups were treated for 14 days.Clinical curative effect was evaluated after treatment.The joint pain score,joint function score and quality of life score of the two groups before and after treatment were compared.The BUA,ESR,DD,IL-1 and CRP of the two groups before and after treatment were compared.The adverse reactions of the two groups during the treatment were compared.Results Total effective rate of observation group was 93.81％ (91/97),which was significantly higher than the control group 73.96％ (71/96),and the difference was statistically significant (x2=14.109,P＜0.01).After treatment,the joint pain (1.1 ± 0.4 vs.2.4 ± 0.8,t=13.984),joint function score (0.7 ± 0.6 vs.1.3 ± 0.9,t=5.451) in the treatment group were lower than those in the control group (P＜0.05).The quality of life score (39.7 ± 9.3 vs.32.5 ± 8.6,t=5.623) in the treatment group were higher than that in the control group (P＜0.05).After treatment,the BUA (302.47 ± 50.72 μmol/L vs.395.21 ± 69.63 μmol/L,t=10.584),ESR (11.63 ± 4.05 mm/h vs.24.87 ± 10.49 mm/h,t=11.589),DD (0.43 ± 0.21 mg/L vs.0.95 ± 0.71 mg/L,t=6.914),IL-1 (12.03 ± 2.62 ng/L vs.19.31 ± 3.25 ng/L,t=17.140) and CRP (6.11 ± 2.40 mg/L vs.11.84 ± 5.49 mg/L,t=9.411) in the treatment group were lower than those in the control group (P＜0.05).There was no significantly difference of the adverse reaction rates of the two groups during treatment (x2=0.216,P=0.642).Conclusions Tongfeng decoction combined with celecoxib for treatment of acute gouty arthritis of damp-heat with blood stasis type showed a good efficacy and low adverse reactions,and it can improve the joint function and quality of life,reduce the BUA.
ABSTRACT
AIM To establish HPLC fingerprints of Zhihuang Tongfeng Decoction (Phellodendri chinensis Cortex,Anemarrhenae Rhizoma,Plantaginis Semen,etc.) and to determine the contents of four constituents.METHODS The analysis of aqueous extract of this drug was performed on a 30 ℃ thermostatic Wondasil C18 column (4.6 mm ×250 mm,5 μm),with the mobile phase comprising of acetonitrile-0.5％ acetic acid in a gradient elution manner,and the detection wavelength was set at 245 nm.RESULTS There were sixteen common peaks in the HPLC fingerprints of ten batches of samples with the similarities of more than 0.9.Berberine hydrochloride,mangiferin,geniposidic acid and salvianolic acid B showed good linear relationships within the ranges of 0.636 2-3.575 μg (R2 =0.9999),0.338-1.425 μg (R2 =0.9990),0.6452-2.7188 μg (R2 =1) and0.938 8-5.275 μg (R2 =0.999 3),whose average recoveries were 100.56％,98.35％,100.25％ and 102.11％ with the RSDs of 2.41％,1.17％,1.24％ and 2.02％,respectively.CONCLUSION This accurate,reliable and specific method can be used for the quality control of Zhihuang Tongfeng Decoction.
ABSTRACT
AIM To establish HPLC fingerprints of Zhihuang Tongfeng Decoction (Phellodendri chinensis Cortex,Anemarrhenae Rhizoma,Plantaginis Semen,etc.) and to determine the contents of four constituents.METHODS The analysis of aqueous extract of this drug was performed on a 30 ℃ thermostatic Wondasil C18 column (4.6 mm ×250 mm,5 μm),with the mobile phase comprising of acetonitrile-0.5％ acetic acid in a gradient elution manner,and the detection wavelength was set at 245 nm.RESULTS There were sixteen common peaks in the HPLC fingerprints of ten batches of samples with the similarities of more than 0.9.Berberine hydrochloride,mangiferin,geniposidic acid and salvianolic acid B showed good linear relationships within the ranges of 0.636 2-3.575 μg (R2 =0.9999),0.338-1.425 μg (R2 =0.9990),0.6452-2.7188 μg (R2 =1) and0.938 8-5.275 μg (R2 =0.999 3),whose average recoveries were 100.56％,98.35％,100.25％ and 102.11％ with the RSDs of 2.41％,1.17％,1.24％ and 2.02％,respectively.CONCLUSION This accurate,reliable and specific method can be used for the quality control of Zhihuang Tongfeng Decoction.