Evaluations of antioxidant enzyme activities, total sialic acid and trace element levels in coronary artery bypass grafting patients

Abstract Introduction: In this study, patients before and after cardiac surgery with cardiopulmonary bypass (CPB) and control subjects were evaluated for erythrocyte glutathione peroxidase, catalase and superoxide dismutase enzyme activities, in addition to glutathione, malondialdehyde, serum total sialic acid, lipid-bound sialic acid, total antioxidant status, trace elements and mineral levels. The correlation of these variables with coronary artery disease (CAD) was also assessed. Methods: A total of 30 CAD patients and 30 control subjects were included in the study. CAD patients were divided into three groups: before surgery (BS), first day after surgery (1st day AS) and seventh day after surgery (7th day AS). Results: Malondialdehyde (MDA) and total sialic acid (TSA) levels were significantly higher in CAD (BS) than in the control group (P<0.05, P<0.05). In addition, GSH and TAS levels were significantly lower in the 1st day AS group than in the control group (P<0.001, P<0.01). Moreover, Co, Cu, Mg, Se, V and Zn levels were significantly lower in CAD (BS) group than in the control group (P<0.01, P<0.01, P<0.01, P<0.01, P<0.05, P<0.001). Conclusions: It was concluded that the levels of LDL-C, total cholesterol, triglycerides and CRP significantly associated with parameters, as well as Cu, Ca and SOD activity, should be measured together to monitor CAD. It is also considered that measuring TSA and MDA might be an appropriate choice for biomarkers of CAD.

The significance of Hp antigen combined with serum total sialic acid and gastric mucosal Survivin detection in the diagnosis of gastric adenocarcinoma / 国际检验医学杂志

Objective To explore the significance of Hp antigen combined with serum total sialic acid and gastric mucosal Survivin detection in the diagnosis of gastric adenocarcinoma.Methods Selected a hospital 90cases of patients with gastric adenocarcinoma (malignant group) and 90cases of the digestive system benign disease (benign disease group) and hospital check-up of 60cases of healthy people (healthy control group) as the research object.Comparing the three groups of patients with Hp antigen, serum total sialic acid positive detection rate and the positive rate of Survivin in the gastric mucosa, and to assess individual detection of Hp antigen, serum total sialic acid and the two combined diagnostic sensitivity and specificity of gastric adenocarcinomas.Results Hp antigen, serum total sialic acid positive test results showed that the malignant tumor group were significantly higher than that of benign disease group and healthy control group, benign disease group obviously higher than that of healthy control group, difference between three groups of participants were statistically significant (P<0.05) .The sensitivity of Hp antigen and serum total sialic acid combined in the diagnosis of digestive system was 80.1％, which was significantly higher than that of single test, but the specificity was reduced.Linear correlation analysis showed that there was a positive correlation between Hp antigen and serum total sialic acid and Survivin detection in gastric mucosa, and the correlation was high (r=0.94, P<0.05) .Conclusion The combined detection of Hp antigen and total serum sialic acid is positively correlated with the results of Survivin detection in gastric mucosa, and has a high correlation.It can replace Survivin detection in gastric mucosa as a sensitive index for the diagnosis of gastric adenocarcinoma.