ABSTRACT
In regenerative medicine, nanotechnology plays a vital role in the diagnosis, the delivery of therapeutic drugs and the treatment of many diseases and disorders. Due to the growing need to develop environmentally friendly technologies in material synthesis, the biosynthesis of Iron Oxide Nanoparticles (IOP) has received considerable attention over the past decades. Therefore, the community is now looking back for traditional medicines for various diseases after the practice of allopathic drugs with tremendous side effects. There are an increasing number of biomedical applications for iron oxide nanoparticles; as such uses are essential for in-vitro characterisation and in-vivo to ensure the bio-safety of these particles. The main complication of diabetes due to frequent lipid peroxidation is liver damage and renal dysfunction, but treatment with Leucas aspera has a pronounced effect on these indicators, which protects the organs from further damage. The current research supports the traditional use of Leucas aspera against diabetes mellitus and cancer, as well as the safety, effectiveness and potency of Leucas aspera leaf extract's anti-diabetic and anti-cancer effect in the in-vivo model. The overall results reveal that Leucas aspera has the potential and can be a candidate of choice without side effect.
ABSTRACT
Considering the safety-risk ratio of steroidal contraceptives, the present work was carried out to evaluate ethno-contraceptive use of Cordia dichotoma G. Forst., Boraginaceae, leaves (LCD). Preliminary pharmacological screening was performed on post-coital female albino rats. The leaves extract (LD50 5.50 g/kg bw) showed 100% anti-implantation activity (n=10) at 800 mg/kg dose level. (2-hydroxypropyl)-β-cyclodextrin (BCD) was used as bioavailability enhancer to form LCD-BCD complex, characterized by DLS, SEM and XRD analyses. The LCD-BCD complex (1:1, w/w) exhibited 100% pregnancy interception (n=20) at the dose level of 250 mg/kg and also showed strong estrogenic potential with a luteal phase defect. Qualitative and quantitative phytochemical analyses were carried out. The LCD extract was standardized by a validated HPTLC method and two contraceptive phytoconstituents, apigenin and luteolin were isolated. A detailed pharmacological analyses followed by chronic toxicity study were performed to predict the reversible nature of the developed phytopharmaceutical. The histological and biochemical estimations detected the reversible contraceptive potential after withdrawal. The observations suggested that the developed phyto-pharmaceutical has potential antifertility activity with safety aspects.
ABSTRACT
The purpose of this paper is to provide reference data related to the body weight, food & water consumptions, urinalysis, hematology and serum biochemistry parameters and absolute & relative organ weights obtained from control Sprague-Dawley rats, used in the 4-week and 13-week repeated-dose toxicity studies conducted in our laboratory between 2005 and 2008. The mean, standard deviation, minimum and maximum range values for hematology and serum biochemistry parameters, data of absolute & relative organ weights, and the difference between sexes and study duration of week 4 versus 13 week are presented. The studies were conducted according to "the standards of Toxicity Study for Medicinal Products" (2005) and The KFDA Notification No. 2000-63 'Good Laboratory Practice (GLP)' (2000) issued by KFDA. These data could be used as reference material of Sprague-Dawley rats by conducting the studies to evaluate the toxicological profile of pre-clinical toxicity studies.