ABSTRACT
Objective To investigate the association between HLA-DQ and the susceptibility to toxoplasma among Chongqing Han population.Methods Toxoplasma infected subjects were screened out by cross-sectional survey,then the control subjects were selected through pair matching at the ratio of 1∶1.Polymerase chain reaction and sequence specific primer(PCR/SSP) were used to determine the frequency distribution of HLA-DQ1 and DQ3 in the two groups.Results The gene frequency distribution of DQ3 was increased among the toxoplasma infected individuals as compared with control subjects and the odds ratio(OR) of DQ3 was 4(P0.05).Conclusion HLA-DQ3 may be associated with toxoplasma infection in Chongqing Han population.
ABSTRACT
Objective To determine the levels of anti-Toxoplasma gondii antibody (ATAb) and antisperm antibody (AsAb) in serum samples and interferon-?(IFN-?) in supernatants of peripheral blood cell culture in females with infertility, and to analyze their correlation. Methods ELISA was applied to determine and compare the ATAb and AsAb in serum samples of 182 women with infertility and those of 94 women with normal pregnant history. The level of IFN-? in supernatants of peripheral blood cell culture was also measured and compared by ELISA. Results The positive rates of serum ATAb and AsAb in the cases of infertility were 15.38% and 18.13% respectively, which were significantly higher than those of women with normal pregnant history( 6.38% and 5.32%). The supernatant IFN-? level in infertile women was significantly higher than that of women with normal pregnant history (0.970?0.493) ?g/L vs (0.531?0.274) ?g/L(P