Immunoelectronmicroscopical Study on the Axonal Coexistence of Serotonin and Substance-P of Fetal Nerve Tissue Transplantation into the Transected Spinal Cord of Rats / 체질인류학회지

The purpose of this study was taken after transplantation of fetal serotonin and substance-P containing raphe nuclei into the transected thoracic spinal cord (T9-10) of adult rats, a suspension of cells derived from the mesencephalic and medullary raphe nuclei of 13~15 days embryonic rats were injected upper and lower the transected spinal cord. After survival periods of 15 days to 1 year, the animals were sacrificed and the spinal cord, processed for the localization of serotonin and substance-P immunoreactive neurons in the transplanted spinal cord, was studied using ABC immunocytochemistry. Immunocytochemical analysis revealed the presence of many serotonin and substance-P immunoreactive neurons in the transplants. In the mesencephlic implants, however, the serotonin and substance-P immunoreactive neurons were not co-localized with the same neurons. The serotonin and substance-P nerve fibers were densely distributed in lamina I and II of the dorsal horn and in lamina VIII and IX of the ventral horn of all segments of the spinal cord. The nontransplanted control, spinal cord of the rats showed a total absence of serotonin and substance-P immunoreactive fibers below the section. Immunoelctronmicroscopic anlysis demonstrated the presence of numerous synaptic contacts formed by serotonin and substance-P containing boutons which are most likely emanated from the grafted serotonin and substance-P.

THE EXPRESSION OF CYCLIN DEPENDENT KINASE 11 FOLLOWING TRANSECTED SPINAL CORD INJURY IN RATS / 解剖学报

Objective To explore the expression and distribution of cyclin dependent kinase 11(CDK11) after transected spinal cord injury(tSCI). Methods tSCI was performed at T9 in adult Sprague-Dawley(SD) rats.The rats were sacrificed 1day,3days,5days,7days,14days after operation respectively.Western blotting was used to detect the changes of CDK11 protein expression after tSCI,and the distribution and localization of CDK11 in spinal cord were investigated by immunohistochemical staining and immunofluorescence double staining. Results Western blotting analysis showed the expressions of CDK11~(p58) and CDK11~(p110),which were the two major isoforms of CDK11,increased at first and then had a decrease tendency in both broken ends of the transected spinal cord.CDK11~(p58) was enhanced on the 3rd day and remained high level until the 7th day; while CDK11~(p110) was also increased,on the 3rd day but went down rapidly after the 5th day.Immunohistochemistry staining was indicated that CDK11 was distributed evenly in the normal spinal cord.On the 3rd day,the staining was elevated obviously in both white and grey matter.Immunofluorescence double staining suggested that CDK11 was colocalized with neuronal neclei(NeuN),cyclic nucleotide 3′phosphohydrolase(CNPase) and glial fibrillary acidic protein(GFAP).Conclusion The expression of CDK11 undergoes spatiotemporal changes after tSCI.These alterations may be involved in secondary spinal cord lesion such as neuronal apoptosis and proliferation of glial cells.