ABSTRACT
Objective: To investigate the effect of extraction and reverse extraction conditions on the transfer of isoliquiritin. Methods: The extraction rate of isoliquiritin was used as the index to determine the best composition and concentration of complexing extractant. Taking the reverse extraction rate of isoliquiritin as the index, the species and concentration of the reverse extraction agent were investigated, and finally the technological conditions for the extraction and reverse extraction of isoliquiritin from glycyrrhizin ultrafiltration were obtained. Results: The best complexation extraction condition was: the ratio of TRPO to sulfonated kerosene was 7:93, and the extraction rate of isoliquiritin reached 97.60%. The best reverse extraction agent was 0.26% NaOH aqueous solution, and the reverse extraction rate of isoliquiritin reached 95.40%. Conclusion: Under the optimal conditions of extraction and reverse extraction obtained in this experiment, isoliquiritin can be transferred from glycyrrhizin ultrafiltration to complexing extractant and then to alkaline reverse extraction agent, and finally isoliquiritin can be obtained by extraction and reverse extraction.
ABSTRACT
Objective To establish a complexation extraction and back extraction technology for the separation and purification of liquiritin from Glycyrrhiza uralensis ultrafiltrate. Methods Taking the extraction rate of liquiritin as an index, the optimum composition of complexing extractant was first determined by uniform design, and then orthogonal experiments were used to optimize the conditions of complexing extraction. Taking the back extraction rate of liquiritin as an index, the process conditions for the back extraction of liquiritin were determined by investigating the type and concentration of back extractant. Results The complexation extraction research found that the complexing extractant should be a binary complexing extractant composed of trialkyl phosphine oxide (TRPO) and sulfonated kerosene. The optimum extraction conditions for liquiritin were as follows: TRPO-sulfonated kerosene (9∶91), pH value of G. uralensis ultrafiltrate was adjusted to 4, volume ratio of organic phase to aqueous phase was 1∶1, and average extraction rate of liquiritin reached 99.6%. The study of back extraction process showed that under the condition that the volume ratio of organic phase to back extractant was 1∶1, 17.5 mmol/L NaOH aqueous solution was the best back extractant, and the back extraction rate of liquiritin was 99.3%. Conclusion Under the optimized conditions, the liquiritin in G. uralensis ultrafiltrate can smoothly transfer from the ultrafiltrate to the complexing extractant and then to the alkaline back extractant. The total transfer rate of liquiritin is as high as 98.9%. This paper can provide a new preparation technology for the separation and purification of liquiritin.
ABSTRACT
Objective: To establish a technique for preparing glycyrrhizin acid (GA) by ultrafiltration-complexation extraction. Methods The effect of pH on the extraction rate of GA from ultrafiltrate was investigated in the range of 4-8; The orthogonal test was used to optimize the technological conditions for complexation extraction of GA; The extraction conditions of GA were determined by investigating the type and concentration of the extractant. Results The optimum extraction conditions for GA were as follows: pH value of Glycyrrhiza uralensis ultrafiltrate was adjusted to 2, TRPO-sulfonated kerosene (5:95, volume percent), volume ratio of organic phase to aqueous phase was 1:1, and average extraction rate of GA reached 99.2%. The best back extraction conditions for GA were as follows: 22.5 mmol/L NaOH aqueous solution was used as the stripping agent, the volume ratio of organic phase to stripping agent was 1:1, the single back extraction rate of GA reached 98.8%, and the total transfer rate of GA was 98.1%. Conclusion Ultrafiltration-complexation extraction technology can be used as a new process for the preparation of GA.