ABSTRACT
O objetivo deste estudo foi avaliar a influência da testosterona sobre a atividade funcional de macrófagos murinos residentes frente ao Trichophyton mentagrophytes. Nas condições ensaiadas, a testosterona influenciou a liberação da H2O2 levando a redução da atividade microbicida dos macrófagos, facilitando o crescimento e diferenciação dos conídeos fagocitados.
The aim of this study was to evaluating the influence of male sexual hormone on activity of Swiss mice resident macrophages cocultured with Trichophyton mentagrophytes. In assayed conditions, testosterone influenced H2O2 release, leading to inhibition of killed macrophage activity in ingested conidia, facilitating its growth and differentiation inside macrophage.
Subject(s)
Animals , Mice , Macrophages, Peritoneal , Testosterone , Tinea , Phagocytosis , Hydrogen PeroxideABSTRACT
BACKGROUND: Dermatophytoses are infections of keratinized tissues, that is, the epidermis, hair and nails, caused by a group of specialized fungi, the dermatophytes. Laboratory diagnoses of dermatophytes such as Tricophyton, Microsporum and Epidermophyton are made by microscopic examination and in vitro culture but they are either time consuming of lacking specificity. OBJECTIVE: In order to develop and apply more rapid and precise diagnostic tests for fungal pathogens to facilitate the improved identification of dermatophytes, we investigated random amplified polymorphism DNA for classification and identification of dermatophytes. METHODS: Amplification reactions were performed in volumes of 5011 containing 10mM Tris-HCl(pH 8.3), 50mM KCl, 1.5mM MgCl2, 0.01% (w/v), gelatin, 200mM dNTP mixture, 50pM primer, Taq polymerase (0.025 units/ microliter), DNA 0.001 microgram/microliter. The optimal condition to. PCR was 2 cycles (denaturing 94 degrees C 2min, annealing 33 degrees C 2min, extension 72 degrees C 4min), 40 cycles, and extension (72 degrees C 10min). RESULTS: RAPD showed interspecies polymorphism in but it had identical patterns in intraspecies. CONCLUSION: It was confirmed that RAPD PCR analysis with optimal conditions is a fast, economical and reproducible method for identification and classification of dermatophytes isolates.