ABSTRACT
Objective To observe the connections between the glutamic acid decarboxylase\|like immunoreactive(GAD\|LI) axonal terminals and the positive neurons showing phosphate actived glutaminase(PAG)\|LI or GABA\-A\|receptor ?3 subunit (GABA\-AR?3)\|LI and parvalbumin(PV)\|LI in the mesencephalic trigeminal nucleus(Vme) of the rat. Methods The triple\|immunofluoresence histochemical staining techniques were used in the present study,and stained sections were observed under a confocal laser\|scanning microscope. Results Many neuronal cell bodies through the whole rostrocaudal extent of Vme were showed dense GABA\-AR?3\|,PAG\| and PV\|like immunoreactivities,respectively.The majority of them were large pseudounipolar neurons(diameter 25\|50?m).About 90% of PAG\|LI neurons expressed both GABA\-AR?3\|LI and PV\|LI.Confocal laser\|scanning microscope further revealed that dense GAD\|LI axonal varicosities were found to surround the somata of Vme neurons showing both GABA\-AR?3\| and PAG\|like immunoreactivities,and formed the close contact each other.Conclusion\ The present results suggested that the GABAergic axonal terminals projecting to Vme might exert inhibitory regulation and control effect through GABA\-AR?3 subunit localizated in somata of Vme neurons on the transmission of orofacial region proprioceptive sensory information mediated by glutamate.
ABSTRACT
Objective The immunocytochemical localizations of GABA\-A\| and GABA\-B\|receptor in the mesencephalic trigeminal nucleus(Vme) of the rat were examined in order to provide morphological evidence for a possible functional analysis of GABA\|receptor in the transmission and modulation of the orofacial region trigeminal proprioceptive sensory information. Methods An immunocytochemical staining method was used in the present study,and immunostained sections were observed under light microscope. Results Many neuronal cell bodies,fibers and terminals with dense GABA\-A\| and GABA\-B\|receptor\|like immunoreactivity(\|LI) were observed to be localized in Vme,and different subunits of GABA\|receptor\|LI showed the morphological difference in their distribution:1.Immunoreactivity for GABA\-B R1 subunit was intensely distributed in the cell bodies of Vme neurons,whereas the weak immunoreactivity for GABA\-BR2 subunit was only observed in the region surrounding the somata of Vme neurons which showed negative GABA\-BR2\|LI;2.A considerable number of GABA\-A\|like immunoreactive neurons,fibers and terminals were also found in Vme.It was involved four kinds of subunits(?1,?2,?3 and ?).The heavy immunoreactivity for GABA\-AR?1 and GABA\-AR?3 subunits were only seen in the cell bodies of Vme neurons.On the other hand,the immunoreactivity for GABA\-AR?2 and GABA\-AR? subunits was only observed in the fibers and terminals in the region surrounding the somata of Vme neurons,which showed negative immunoreactivity for above subunits;3.Dense GABA\-BR2\|,GABA\-AR?2\| and GABA\-AR?\|LI fibers and terminals surround the somata of Vme neurons,especially for GABA\-AR?2\|LI.Conclusion\ The present results suggest that there are some differences in the distribution of the various subunits of GABA\-A\| and GABA\-B\|receptor in Vme.It is likely that the inhibitory regulation and control effects of GABAergic terminals on the transmission of orofacial region proprioceptive sensory information might be mainly mediated through GABA\-AR?1 and GABA\-AR?3 subunits in somata of Vme neurons.