ABSTRACT
Objective To study the chemical constituents in 95% ethanol aqueous extract of Trigonostemon lutescens. Methods The open silica gel, MCI, Sephadex LH-20 column chromatography, and the semi-preparative HPLC were used to isolate and purify the chemical constituents from the EtOAc fraction of T. lutescens. The structures of the isolates were elucidated by their physiochemical properties, NMR, and MS spectroscopic data, as well as comparison with literature data. Results Eleven compounds were isolated from the EtOAc fraction of the 95% aqueous EtOH extract of T. lutescens, and their structures were identified as seven coumarins, auraptenol (1), meranzin hydrate (2), xanthotoxin (3), bergapten (4), isoimpinellin (5), alloisoimperatorin (6), and isodemethylfuropinarine (7); Two phenylalanine glycosides, 3,4-dihydroxy allylbenzene-4-O-β-D-glucopyranoside (8) and 1-O-β-D-glucopyranosyl-4- allylbenzene (9); One phenylethanol, 1-(2-ethylphenyl)-1,2-ethanediol (10); One alkaloid, 8-hydroxy-3-methoxy-5H-pyrido [2,1-c] pyrazin-5-one (11). Conclusion All these compounds are isolated from the genus Trigonostemon for the first time.
ABSTRACT
AIM To study the chemical constituents from twigs of Trigonostemon lutescens Y.T.Chang et J.Y.Liang.METHODS The ethyl acetate fraction of 95％ ethanol extract from T.lutescens twigs was isolated and purified by silica,Sephadex LH-20 and MCI column,then the structures of obtained compounds were identified by physicochemical properties and spectral data.RESULTS Ten compounds were isolated and elucidated as 3-O-acetyloleanolic acid (1),methyl 3-acetoxy-12-enly-oleanen-28-oate (2),taraxerone (3),spiciflorin (4),simiarenol (5),β-sitosterol palmitate (6),3,3',4-tri-O-methylellagic acid (7),β-sitosterol (8),stigamasterol (9),palmitic acid (10).CONCLUSION Compounds 1-6 are isolated from genus Trigonostemon for the first time,compounds 7 and 9 are first obtained from this plant.
ABSTRACT
Objective: To study the daphnane diterpenoids of Trigonostemon lutescens form Guangxi province. Methods: The ethanol extract of T. lutescens was separated and purified by column chromatography, and the structures of the compounds were identified by physicochemical properties and spectral data. Results: Twelve daphnane diterpenoids were separated and identified form T. lutescens as trigoxyphin A (1), 3,16α-dihydroxy-kaurane (2), entkaurane-3,16α-diol (3), 10,16α-dihydroxy-isokaurane (4), entkaurane-3, 5,16α-triol (5), 8α-hydroxy-3,4-secopimara-4,15(18)-diene-3-oic acid (6), 8α-hydroxy-3,4-secopimara-4,15(18)-diene-3-oate (7), 3,4-secopimara-4,8(9),15(18)-triene-3-acid-methyl ester (8), trigoflavidone A (9), clerodane-2,4,11-triene-17-acid methyl ester (10), 3α-dihydroxy-kaurane-16-ene (11), and 13α-dihydroxy-kaurane-16-ene-18-acid methyl ester (12). Conclusion: All these daphnane diterpenoids are first separated and identified form T. lutescens.