ABSTRACT
The economic losses associated with diseases caused by Trypanosoma congolense and the devastating effect of Ancylostoma caninum (A. caninum) in dogs’ necessitated the present study. Sixteen dogs grouped into 4 of 4 members each were used in the study. GROUP I was uninfected dogs (control), GROUP II was infected with Trypanosoma congolense (T. congolense) infection, GROUP III was mixed infections of Trypanosoma congolense and Ancylostoma caninum (T. congolense /A. caninum) and GPIV was infected with Ancylostoma caninum. At first Ancylostoma caninum infection was done on GPIII and GPIV. Two weeks later T. congolense infections was done on GPII and superimposed on GPIII. Three weeks post trypanosome infection; GPII and GPIII were treated with diminazene aceturate. Mebendazole was used on GPIII and GPIV and treatment repeated 2 weeks later. The prepatent period of T. congolense infection was 14.00±1.40 days in single infection and 9.00±1.10 days in conjunct infection of T. congolense and A. caninum. Persistent parasitaemia resulted in repeated treatment with diminazene aceturate at 7 mg/kg and mebendazole at 100mg twice daily for 3 days. The predominant signs revealed include; lethargy, vomition, enlargement of popliteal lymphnodes, pyrexia, oedema of fore and hind limbs and ocular discharges, anaemia, and slight emaciation. The symptoms were more severe in GPIII compared to GPII and GPIV. The egg per gram of faeces (EPG) in (GPIV) was significantly higher than the conjunct infection (GPIII). Treatment only slightly improved clinical manifestations. In conclusion, conjunct infections of T. congolense / A. caninum would result to more severe disease condition than in single infection of either disease in dogs. The severity of symptoms of the diseases were more in conjunct T. congolense / A. caninum as evidenced by high mortality compared with the single infections. Therefore symptoms of the diseases could serve as a surrogate diagnostic tool in diagnosis and vigorous treatment of infected dogs.
ABSTRACT
The effects of experimental Trypanosoma congolense infection on the ejaculate of rabbits and changes caused after treatment with Diminaveto® were investigated using 24 New Zealand White rabbits (bucks). The bucks were housed singly in standard rabbit cages and fed on specialized ration containing 10 percent Protein supplement, grains, legume, salt and fresh water ad libitum during the study. Data on ejaculate characteristics were collected from all the bucks in the first phase (i.e. before infection) and in the second phase (i.e. during infection, with 4.8x10(5) Trypanosoma congolense, intraperitoneally). Similar data were collected from 12 randomly selected bucks treated with 7.0mg/kg Diminaveto® following reconstitution during the third phase. Data collected were analysed using the Paired T- Test and Analysis of Variance. The infection led to significant (P< 0.05) reduction in spermatozoa motility, concentration and mass activity, with a significant (P< 0.05) increase in percentage of sperm cells with morphological abnormalities. Treatment with Diminaveto® led to improvement in all ejaculate parameters investigated. However, it was observed that the ejaculate did not attain the "before-infection" status following treatment with Diminaveto®. The study showed that infection with Trypanosoma congolense in rabbits caused significant reduction in ejaculate characteristics. Treatment with Diminaveto® however led to improvement in the ejaculate though at a rate slower than that at which the infection caused the reduction.
Fue estudiado en 24 conejos Nuevo zelandeses blancos, machos, los efectos de la infección experimental de Trypanosoma congolense sobre la eyaculación y los cambios producidos después del tratamiento con Diminaveto® . Los machos fueron colocados individualmente en jaulas de conejos estándar. Durante el estudio fueron alimentados con ración especializada, con 10 por ciento de suplemento de proteínas, granos, legumbres, sal y agua fresca ad libitum. Los datos sobre las características de la eyaculación se obtuvieron de todos los machos en la primera fase (es decir, antes de la infección) y en la segunda fase (es decir, durante la infección, con 4,8x10(5) Trypanosoma congolense, por vía intraperitoneal). Datos similares se obtuvieron de 12 machos al seleccionados al azar, tratados con Diminaveto® 7,0mg/kg después de la reconstitución durante la tercera fase. Los datos fueron analizados mediante t de student y análisis de varianza. La infección fue significativa (P <0,05) habiendo reducción de la motilidad de los espermatozoides, la concentración y actividad de masas, con un efecto significativo (P <0,05) aumento en el porcentaje de espermatozoides con anomalías morfológicas. El tratamiento con Diminaveto® condujo a una mejoría en todos los parámetros investigados del eyaculado. Sin embargo, se observó que el eyaculado no alcanzó el "antes de la infección" tras el tratamiento con Diminaveto®. Además, el estudio mostró que la infección con Trypanosoma congolense en conejos causó una reducción significativa en las características del eyaculado. Sin embargo, el tratamiento con Diminaveto ® condujo a una mejoría en la eyaculación aunque a un ritmo más lento que en la infección causada por la reducción.