ABSTRACT
Objective:To investigate five kinds of tuberculin skin test (TST), tubercle bacillus-antibody(TB-Ab), interferon-γ release assay(IGRA), tubercle bacillus-DNA (TB-DNA) and collection of bacterial centrifugal smears methods, the application value of combined detection in improving the diagnostic efficiency of pneumoconiosis complicated with tuberculosis.Methods:A total of 150 eligible patients with pneumoconiosis from January 2018 to January 2019 in Zhangjiakou Pulmonary Hospital were selected as the research subjects, and all of them underwent TST, TB-Ab, IGRA, TB-DNA and bacterial centrifugal smear detection. Compared the positive rates of five detection methods in pneumoconiosis and its different stages, and compare the proportion of tuberculosis infection and tuberculosis in different stages of pneumoconiosis.Results:Among the 150 patients with pneumoconiosis, 41 cases (27.33%) were with pneumoconiosis complicated with tuberculosis infection, 24 cases (16.00%) with pneumoconiosis complicated with clinically diagnosed pulmonary tuberculosis, 21 cases (14.00%) with pneumoconiosis complicated with confirmed pulmonary tuberculosis, and 45 cases (30.00%) with pneumoconiosis complicated with pulmonary tuberculosis; with the improvement of pneumoconiosis stage, the proportion of pneumoconiosis combined with tuberculosis infection and pulmonary tuberculosis increased significantly ( P<0.05). Compared with TB-Ab, TB-loop-mediated isothermal amplification(LAMP), and interlayered cup collection centrifuge smear method, the overall positive rate of IGRA detection and pneumoconiosis stage Ⅲ were higher ( P<0.05), but there was no significant difference compared with TST detection ( P>0.05). The positive rate of combined detection was higher, but there was no significant difference compared with IGRA detection ( P>0.05). With the increase of pneumoconiosis stage, the positive reaction intensity of TST decreased, and the positive value of TB-Ab and IGRA increased. Conclusions:The combined detection of TST, TB-Ab, IGRA, TB-DNA and bacterial centrifugal smear method can significantly improve the diagnostic efficiency of pneumoconiosis combined with tuberculosis.
ABSTRACT
OBJECTIVE: To explore the effect of Radix Ranuncoli Ternati on the expression of granule lytic peptide and bactericidal ability of T lymphocyte in peripheral blood lymphocytes of patients with pulmonary tuberculosis. METHODS: Peripheral blood lymphocytes were treated with Ranunculus. MTT assay was used to detect cell proliferation. Real-time PCR was used to detect the mRNA level of GLS in patients. RESULTS: Radix Ranuncoli Ternati could enhance expression of Tubercle bacillus. The RESULTS of MTT assay indicated that the rate of peripheral blood lymphocytes apoptosis inducted by the Radix Ranuncoli Ternat. The Western blotting RESULTS showed that Radix Ranuncoli Ternati made the expression levels of GLS in peripheral blood lymphocyte increasing. When the concentration of the drug is 200 mg·L-1, it can effectively induce the activation of tuberculosis resting bacteria. The expression of GLS and the level of mRNA were increased in peripheral blood lymphocytes. CONCLUSION: Radix Ranuncoli Ternati may increase the expression of GLS and enhance the body's killing effect on pathogenic bacteria to achieve the therapeutic effect.
ABSTRACT
Tuberculosis is one of the critical diseases with priority of the State's infectious disease prevention.This research,based on the collection and analysis of the cases cured by "feilaokang" and a fine result for symptomatic treatment experiment in vitro,make a further research of bacteriostasis in vivo.The pathological section examination has also been made,including HE coloration and Z-N coloration examination.The result indicate that there is a clear effect,for some indicators,on infected mouse for middle does and a clear effect,for all indicators,on infected mouse for high does.This result would be the very foundation for the further research of screening for MDR-TB with effect by "feilaokang" and concluding for the antibacterial coverage and antibacterial material basis.