ABSTRACT
Single cell sequencing developed in recent years,which studies genome and transcriptome at single cell level,is more suitable for solving problems on minor special sample studies,heterogenous population analysis and finding concurrent or mutually exclusive genomic events,compared to bulk sequencing.For breast cancer,which is highly heterogeneous,bulk sequencing data is not enough for solving many clinical problems,and single cell sequencing precisely plays an important role in it.This review,focusing on a minor special cell population in breast cancer (such as cancer stem cells,circulating tumor cells,et al),tumor heterogeneity and clonal evolution,and chemotherapy resistance,summarized application of single cell sequencing in breast cancer research in recent years,and discussed the future research directions.