ABSTRACT
Objective To prepare thermosensitive in sitt hydrogels (ISG) of gabexate mesilate and establish quality control method. Methods ISGs were prepared using PF127 and PF68 as matrix, evaluated by gelling temperature, gelling time, gelling capacity in vitro, and determinated by HPLC. Results The gelling time of ISG was (1. 8 ±0. 2) min, with gelling temperature at 317. When the gel temperature rose to 31 7, the viscosity of ISG increased dramatically. The linear range of HPLC determination curve was 10 -350 μg/ml, with an average recovery of 99. 63% (RSD =0. 88%). Conclusion Preparation of the gel with controllable quality is simple and easy. The determination method is reliable for gabexate mesilate thermosensitive m sitt hydrogels.
ABSTRACT
Objective To prepare thermosensitive in situ hydrogels (ISG) of gabexate mesilate and establish quality control method.Methods ISGs were prepared using PF127 and PF68 as matrix, evaluated by gelling temperature , gelling time, gelling ca-pacity in vitro, and determinated by HPLC.Results The gelling time of ISG was (1.8 ±0.2)min, with gelling temperature at 31℃. When the gel temperature rose to 31℃, the viscosity of ISG increased dramatically .The linear range of HPLC determination curve was 10-350 μg/ml, with an average recovery of 99.63%( RSD=0.88%) .Conclusion Preparation of the gel with controllable quality is simple and easy .The determination method is reliable for gabexate mesilate thermosensitive in situ hydrogels .