ABSTRACT
Thymic stromal lymphopoietin(TSLP)can enhance the secretion of type 2 cytokines by type 2 T helper(Th2)cells and type 2 innate iymphocytes(ILC2s)in the lung through direct and indirect action.In terms of indirect effects, TSLP can promote the proliferation and expansion of naive CD4 + T cells by using dendritic cells, and promote the recruitment of Th2 cells to the initial inflammatory site.It can also use stromal mononuclear/macrophages cells to start the acute primary Th2-dependent immune response.In the aspect of direct effect, TSLP can activate ILC2s before the adaptive immune response is initiated, and also can use immature T cells, effector Th2 cells and memory Th2 cells to participate in the adaptive immune response.Therefore, TSLP plays an important role in allergic immune response of lung.
ABSTRACT
Aim To reveal the effects of Guizhi decoction (G Z D) on allergic contact dermatitis by establishing the mouse allergic contact dermatitis model induced by fluoresciniso thiocyate (FITC) and culturing splenic lymphocytes. Methods BALB/c mice were sensitized by smearing the 1.5% FITC on the shaved abdomens on day 1 and day 2 and elicited on the right ear with 0. 6% FITC solution on day 6. GZD was administered from day 1 to day 7. Ear thickness was measured 24 h after elicitation. HE staining was performed for pathohistological examination. Interleukin-4 (I L - 4), IL-5, IL-9, interferon γ(IFN-γ) in ear tissue homogenates and IgE in serum were detected. The splenic lymphocytes were stimulated with ConA. Results The ear swelling was significantly suppressed by administering GZD. GZD reduced the infiltration of inflammatory cells and edema in the ear, and the levels of I L 4, IL-9, IFN7 and IgE. GZD could also reduce the expression of I L 4, IL-10, IFNγ and GATA3. Conclusions GZD could suppress allergic contact dermatitis elicited by FITC, which may result from inhibiting the differentiation of lymphocytes to Th2 cells.