ABSTRACT
Objective To investigate the expression of UCH37 in cervical cancer tissue,and the associa-tion of UCH37 expression and clinicopathological features of cervical cancer patients,and detect the effect of UCH37 on cell proliferation,cell apoptosis and cell cycle of HeLa cells. Methods Quantitative real-time poly-merase chain reaction(qRT-PCR)was conduct to detect the expression of UCH37 mRNA in cancer tissue and the adjacent tissue of cervical cancer patients. Spearman′s rank test was conduct to analyze the clinical relevance of UCH37 in cervical cancer. Cell count kit-8 was performed to detect the effect of UCH37 on proliferation of HeLa cells. Flow cytometry was performed to detect the effect of UCH37 on cell apoptosis and cell cycle of HeLa cells. Results qRT-PCR results revealed that UCH37 was upregulated in cervical cancer tissue compare to the adjacent tissue.Spearman rank correlation analysis showed that high UCH37 expression was significantly associated with FI-GO stage of cervical cancer patients.CCK-8 assay results showed that cell proliferation was inhibited in HeLa cells transfected with UCH37-siRNAs,and flow cytometry assays revealed that cell apoptosis was promoted in HeLa cells transfected with UCH37-siRNAs.Furthermore,compared with HeLa cells transfected with UCH37-NC,HeLa cells transfected with UCH37 siRNAs were arrested at G1 stage.Conclusion UCH37 expression is upregulated in tissue of cervical cancer,and UCH37 could promote cell proliferation of HeLa cells.UCH37 is a potential therapeu-tic target in cervical cancer.
ABSTRACT
Objective To explore the expression and cilinical significance of UCH37 in colon cancer, and detect the effects and underlying mechanism of UCH37 on cell proliferation and cell apoptosis of colon cancer cell lines. Methods Expressions of UCH37 were analyzed by immunohistochemistry in colon cancer patients. Spearman′s rank test was conducted to analyze the clinical relevance of UCH37 in colon cancer. QPCR was conducted to detect the expression of UPS39 in colon cancer cell lines and NCM460 cells.CCK-8,flow cytometry, co-immunoprecipitation were conducted to detect the effects of UCH37 on cell proliferation,cell apoptosis. And ubiquitinated level of MIF. Results Compare to adjacent tissues,immunohistochemistry and chi square analysis revealed that the positive rate of UCH37 was upregulated in colon cancer tissues.Spearman rank correlation showed that positive UCH37 expression was significantly associated with TNM stage and cell differentiation of colon cancer patients. CCK-8 results showed cell proliferation in colon cancer cells transfected with UCH37 NC was promoted and cell apoptosis in colon cancer cells transfected with UCH37 NC was inhibited compared with cells transfected UCH37 siRNAs;furthermore,compared to cells transfected lentiviral vector carrying pLenti6.3.cell proliferation in colon cancer cells transfected with lentiviral vector carrying UCH37 was promoted and cell apoptosis in colon can-cer cells transfected with lentiviral vector carrying UCH37 was inhibited.Co-IP showed MIF could be deubiquitinat-ed by UCH37 in colon cancer cells. Conclusion UCH37 expression is upregulated in colon cancer,and UCH37 could promote cell proliferation of colon cancer cells by deubiquitinating MIF.