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Objective To compare the contents of total flavonoids, forsythoside A and phillyrin in Forsythiae Fructus leaves tea under different drying conditions; To determine the best drying method for Forsythiae Fructus leaves tea. Methods With the sodium nitrite-aluminum trichloride-sodium hydroxide solution as color reagent, total flavonoids in forsythiae Fructus leaves tea were determined by UV spectrophotometry at the wavelength of 500 nm. HPLC was used to determine the contents of forsythiaside A and phillyrin. The analysis was performed on a Diamosil C18 (2) column (4.6 mm × 250 mm, 5 μm); the mobile phase was composed of acetonitrile and 0.4% acetic acid with gradient elution; the detection wavelength was set at 277 nm; the flow rate was 1.0 mL/min at column temperature of 30 ℃. Results The content of total flavonoids of Forsythiae Fructus leaves tea under different drying conditions was basically the same; the sequence of the contents of forsythoside A and Forsythin of Forsythiae Fructus leaves tea under different drying conditions was: ventilated drying > vacuum drying > blast drying. Conclusion Different drying conditions have no effect on the contents of total flavonoids in Forsythiae Fructus leaves tea, but have obvious effects on the contents of forsythiaside A and phillyrin. Ventilation shade is better than blast drying and vacuum drying for preverence of forsythiaside A and forsythin.
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OBJECTIVE:To study the effect of moxifloxacin on pharmacokinetics of phenytoin sodium in rabbits. METHODS: A total of 10 rabbits were injected with phenytoin sodium (10 mg?kg-1) via the vein in ear edge,with serum samples taken at 10,30,60,120,240,360,and 480 min,respectively for establishing phenytoin sodium alone group. 48 h after the last sampling,the rabbits were given oral moxifloxacin (10 mg?kg-1) for 5 consecutive days;on day 6,the rabbits were injected with phenytoin sodium (10 mg?kg-1) at 2 h after oral administration of moxifloxacin,then serum samples were taken at different time points for establishing combination group of phenytoin sodium and moxifloxacin. The serum concentrations of phenytoin sodium at different time points were determined by UV-spectrophotometry and the pharmacokinetic parameters were computed with 3p97 program. RESULTS: The plasma concentration of phenytoin sodium reduced in the combination group compared with alone group. The AUC in the two groups was (5 836.22?489.13) vs. (4 329.21?344.67) mg?min?L-1 (P0.05). CONCLUSION: The elimination of phenytoin sodium was significantly accelerated by combination with moxifloxacin.
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Objective To study the qualitative analysis and quantitative analysis of flavonoids and other ingredients in leaves of Lonicera hypoglauca miq,and to optimize the reflux extraction condition for total flavonoids from leaves of lonicera hypoglauca miq. Methods Preliminary test was used for qualitative analysis. UV-spectrophotometry was used to determinate content of total flavonoids. By orthogonal design,the extraction rate of flavonoids was determined in different factors of ethanol reflux extracting. Results Leaves of lonicera hypoglauca miq contained alkaloids and flavonoids.The optimum conditions of ethanol reflux extracting total flavonoids are 12 times 50% ethanol,and refluence for 1.0h at water bath for two times.According to validate experiment,the average total flavonoids content was 16.6% in leaves of lonicera hypoglauca miq,with RSD=1.52%(n=3). Conclusion Flavonoids are in higher level in leaves of Lonicera hypoglauca miq. There are some chemical similarity in leaves of lonicera hypoglauca miq and leaves of flos lonicerae.
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OBJECTIVE:To establish the Second-order Derivative UV-spectrophotometry for the determination of transdermal osmolality of metoprolol tartrate (MP)in vitro.METHODS:The quantitation of the vibration amplitude (?A)between the characteristic valley and the characteristic peak of MP appeared at 224nm and 234nm in UV-scanning.RESULTS:The linear concentration range of metoprolol tartrate was 5～60?g/ml,the average recovery was 98.89%with average RSD at 2.05%.CONCLUSION:This method can accurately determine the transdermal osmolality of MP in vitro yet without the interference of skin exudates,its operation is simple and fast and the results are accurate and reliable.
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OBJECTIVE:To study the compatible stability of foscarnet sodium injection with5%glucose injection,10% glucose injection,glucose saline and NaCl injection.METHODS:The content changes of foscarnet sodium injection were determined by UV-spectrophotometry at different time within8h after mixing with 4 kinds of infusion solutions,the appearance description of the solution was observed,the pH value and the UV scan spectra changes of foscarnet sodium were determined.RESULTS:No marked changes were noted in the contents,appearance,pH value and the UV-scan spectra of the mixed solutions.CONCLUSION:Foscarnet sodium injection can be used with in 8h after mixing with 4 infusion solutions under the room temperature.
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OBJECTIVE:To establish UV-spectrophotometry method for the determination of melatonin in the compound melatonin capsules.METHODS:The UV-spectrophotometry was adopted with the detection wavelength at277nm.RESUL_ TS:There was a good linear relationship between melatonin and its absorbability(r=0.9996)within the concentration range of5～25?g/ml.The average recovery was96.49%(RSD=1.21%,n=6).CONCLUSION:The method is accurate,reliable,convenient and stable.
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OBJECTIVE:To prepare Fluconazole adhesive gel.METHODS:Using carbopol934p,glycerine and other inor?ganic solvents as adjuvant,the Fluconazole adhesive gel was prepared.Then the pharmaceutic study and quality standard es?tablishment were carried out.RESULTS&CONCLUSION:The Fluconazole adhesive gel was simple in preparation and stable in property.The adhesive gel may become an ideal antifungal preparation for topical application.
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OBJECTIVE:To study the influence of different herb composition on content of aconitine in Rhubarb and Aconite Decoction.METHODS:On the basis of Rhubarb and Aconite Decoction,different prescriptions were made up and different de?cocting technics were adopted,then the content of aconitine was determined with UV-spectrophotometry.RESULTS:The content of aconitine in mixture of decoctions of single herb decreased about20%and the content of aconitine in the decoction of combined herbs decreased about45%compared with that in decoction of single Aconite.Combination of Asarum with Aconite showed no influence on the content of aconitine.CONCLUSION:Rhubarb can control the toxicity of Aconite in Rhubarb and Aconite Decoction.
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OBJECTIVE:To prepare indapamide capsules and study the quality control methods METHODS:The content of indapamide in capsules was determined by UV-spectrophotometry The other ingredients were determined by HPLC RES_ULTS:The calibration curve was linear within the concentration range of 3 7～11 1?g/ml(r=0 9 997);the recovery of indapamide was 99 50%,RSD=0 43% The RSDs of indapamide and other ingredients were 0 43% and 0 1% respectively The relative percentage contents of 3 batches of sample were 99 07%,99 34%,99 58% respectively The uniformity A+1 80S of contents of indapamide in all samples detected was
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OBJECTIVE:To study the determination methods for semifinished material and tablet of ibudilast by HPLC and UV-spectrophotometry METHODS:The detections were performed by HPLC and UV-spectrophotometry at wavelength 318nm RESULTS:The linear range of ibudilast was 10～50?g/ml by HPLC method with an average recovery of 101 8% and RSD 2 1%(n=5);The linear range of ibudilast was 10～50?g/ml by UV-spectrophotometry with an average recovery of 101 6% and RSD 2 1%(n=5) CONCLUSION:Both methods are accurate,simple and rapid and suitable for the determination of ibudila_st
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OBJECTIVE:To establish the quality control standard of anticancer herbal suppository METHODS:The polygonaceae and cantharidin were qualitatively and quantitatively detected by TLC and UV-spectrophotometry respectively RESU_LTS:TLC was suitable for qualitative analysis of polygonaceae and UV-spectrophotometry for quantitative analysis of cantharidin CONCLUSION:TLC and UV-spectrophotometry can be used in quality control of this preparation
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AIM: To study the pharmacokinetic characteristics of domestic ofloxacin tablet. METHODS: A method of deter- mination of drug concentration in urine by UV-spectrophotometry was established. RESULTS: K = 0. 1 278/h; T1, 2 = 5. 96h, Xu0-24 = 165. 84mg; (Q) = 82. 84%. CONCLUSION: This method is simple and rapid, it is suitable for pharmacokinetics study and clinical monitoring.
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OBJECTIVE: To establish a new method for measuring the content of benzalkonium bromide in disinfectant so- lution METHODS: The sodium nitrite was removed from disinfectant solution by reaction between sodium nitrite and urea to form N2 and CO2 and then benzalkonium bromide in the solution was directly determinated by UV-spectrophotometry with no need of separation RESULTS:There was no significant difference between this method and the method in pharmacopeia CON CLUSION:The method is simple, rapid and accurate and can be used to measure benzalkonium bromide in the solution.
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OBJECTIVE:To establish UV-spectrophotometry method in the determination of metformin hydrochloride in compound metformin hydrochloride tablets.METHODS:The determination was carried out with water as the solvent under the detection wavelength of233nm.RESULTS:There was a good linear relationship between metformin hydrochloride and its absorbability(r=0.9999)within the concentration range of1～10?g/ml.The average recovery was99.3%with RSD at0.24%(n=9).CONCLUSION:The method is accurate,fast,convenient,and which can be used to determine the contents of metformin hydrochloride in compound metformin hydrochloride tablets directly without isolation.
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Icariin and total flavonoids in "Xianlinggubao Capsule", a Chinese herbal preparationcontaining Epimedium L. as the main ingredient for the treatment of osteoporosis, were determined quan-titatively by HPLC and UV spectrophotometry respectively. It was found that the contents of icariin were4.16～27.67 mg/g and that of flavonoids were 62. 56～109.78 mg/g. The recoveries were 96.17% and103.58% respectively. The method could be used for the quality control of the drug.
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AIM To establish a UV spectrophotometry for the determination of arsenic in Jiuji Xingjun Capsules. METHODS Arsenic was transformed into hydrogen arsenide,then silver diethyldithiocarbamate was reduced for colloid silver by hydrogen arsenide,its absorbance was determined at the wavelength of 507 nm. RESULTS The linearity range of curve was 0.5-4.5 ?g?mL -1 ( r =0.999 5, n =7),The average recovery was 100.11 % ( RSD =1.64 T《B》 % , n =15). CONCLUSION This method is rapid and accurate.
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OBJECTIVE:To evaluate the quality of Artemisia annua L. samples planted in different site condition in Chongqing Wulong county and to provide a fast and convenient assaying of artemisinin in Artemisia annua L. samples in the industrial development of this Chinese herbal medicine. METHODS: The Artemisia annua L. planted in different site condition in Chongqing Wulong county were sampled during harvesting time, which were subjected to many processing steps such as drying by baking, crushing, microwave extraction and color reaction etc. Then the absorbance of the test liquid was detected by UV-spectrophotometry at a wavelength of 290 nm. Finally Artemisinin content was computed based on the regression curve of standard solution and the quality of the Artemisia annua L. samples was evaluated. RESULTS: The liner concentration range of Artemisinin was 8～40 mg?mL-1 (r=0.999 8) with an average recovery rate of 99.98% (RSD=1.48%, n=9).CONCLUSION: The results showed that site condition did have effect on the quality of Artemisia annua L. to some degree. UV-Vis method is simple, rapid and reproducible, and suitable for the quality control of Artemisia annua L. samples.
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OBJECTIVE:To establish a method for the assaying of total flavonoids in the extracts of Gynostemma and to provide basis for the study of its internal quality.METHOD:The contents of total flavonoids in6kinds of Gynostemma made from Guangxi were determined by UV-spectrophotometry with a detection wavelength at510nm.RESULTS:Good linear re-lationship with absorbability occurred when the concentration range of rutin was0.0092～0.0553mg/ml(r=0.9999,n=6)and its average recovery was100.8%(RSD=2.25%,n=6).CONCLUSION:The method is simple and accurate,of the testing samples,Gynostemma from Guangxi ranked the first in terms of the content of total flavonoids.
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OBJECTIVE:To simultaneously determine the contents of CIPRO and MNZ in compound ciprofloxacin ear dro_ ps by nodical polyploid UV-spectrophotometry.METHODS:CIPRO and MNZ were nodical absorption at wavelength of350.0nm and this was just the sum of isoabsorptive point of two components while DXM did not absorb UV ray at this point.Therefore350.0nm was adopted as the detecting wavelength for these two components.RESULTS:The average recovery and relative standard deviation of CIPRO were99.7%and1.4%respectively.The average recovery and relative standard de?viation of MNZ were100.7%and1.6%respectively.CONCLUSION:The method is accurate,rapid,simple and sensitive.This method can be used to control the quality of this preparation.
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OBJECTIVE:To investigate the dissolubility of commercial metformin hydrochloride tablets from different manufactories.METHODS:The in vitro dissolubility of 14 kinds of commercial metformin hydrochloride tablets was determined by basket method and the dissolution parameters were analyzed with variance analysis method.RESULTS:The in vitro dissolubility of 14 kinds of metformin hydrochloride tablets fitted to the request of ChP2000,but the dissolution parameters were different.CONCLUSION:The statistical results indicate that there are significant differences between products from different factories(P