ABSTRACT
Objective To observe the expression of heat shock protein 70 (HSP70) in lung of rats after paraquat (PQ) poisoning and to investigate the therapeutic effects of ulinastatin. Method Seventy-two adult healthy SD rats were randomly(random number) divided into control group (group A, n = 24), poisoning group (group B, n =24) and ulinastatin group (group C, n =24). The rat models of acute PQ poisoning were established by intra-gastric administration of 80 mg/kg PQ to rats of group B and group C, and the rats of group C were intra-peritoneally injected with 100 000 IU/kg ulinastatin 30 minutes after poisoning. The expression of HSP70 in lung tissue was observed, and W/D and histopathological changes in lung tissue were compared 12 h,24 h,48 h and 72 hours after poisoning. The expression of HSP70 in lung tissue was assayed by using RT-PCR. All quantitative data were processed with one-way analysis of variance to compare multiple sample means. Results Compared with group A, the expression of HSP70 in the lung of rats in group B and group C increased significantly at all intervals ( P ＜ 0.05). The pathological changes in lung tissue of rats with PQ poisoning showed mainly congestion,leukocytes infiltration and local hemorrhage, and the pathological changes in lung tissue of group C were significantly lessened. Conclusions Ulinastatin may ameliorate the acute lung injury to a certain extent after PQ poisoning in rats by enhancing the expression of HSP70.
ABSTRACT
@#BACKGROUND: Paraquat (PQ) is an effective herbicide and is widely used in agricultural production, but PQ poisoning is frequently seen in humans with the lung as the target organ. Clinically pulmonary pathological changes are often used to predict the severity and prognosis of the patients. In this study, we observed the expression of heat shock protein 70 (HSP70) in rat lung after PQ poisoning and to investigate the therapeutic effects of ulinastatin. METHODS: Seventy-two adult healthy SD rats were randomly divided into a control group (group A, n=24), a poisoning group (group B, n=24), and an ulinastatin group (group C, n=24). The rat models of acute PQ poisoning were established by intra-gastric administration of 80 mg/kg PQ to rats of groups B and C, and the rats of group C were intra-peritoneally injected with 100000 IU/kg ulinastatin 30 minutes after poisoning. The expression of HSP70 in lung tissue was observed, and W/D and histopathological changes in the lung tissue were compared 12, 24, 48 and 72 hours after poisoning. The expression of HSP70 in the lung tissue was assayed by using RT-PCR. All quantitative data were processed with one-way analysis of variance to compare multiple sample means. RESULTS: Compared to group A, the expression of HSP70 in the lung of rats in groups B and C increased significantly at all intervals (P<0.05). The pathological changes in lung tissue of rats with PQ poisoning included congestion, leukocytes infiltration and local hemorrhage, whereas those of group C were significantly lessened. CONCLUSION: Ulinastatin may ameliorate acute lung injury to some extent after PQ poisoning in rats by enhancing the expression of HSP70.