ABSTRACT
El uso de las plantas medicinales como una alternativa para tratar dolencias y/o enfermedades, está tomando gran importancia para la creación e investigación de nuevas formulaciones. Objetivo: Determinar la actividad cicatrizante y toxicidad dérmica del extracto etanólico de los tubérculos de Ullucus tuberosus Caldas "olluco" en animales de experimentación. Métodos: La especie vegetal, fue recolectada en la comunidad de Panao departamento de Huánuco, Perú. Se realizó una extracción etanólica del tubérculo del Ullucus tuberosus Caldas "olluco" para el análisis fitoquímico. La actividad cicatrizante, se realizó con la técnica de Vaisberg y col, donde se preparó tres cremas a base de la especie vegetal, a concentraciones 0.5, 5 y 10%, éstas fueron comparados con dos grupos estándares, crema al 1% de Croton Lechleri "Sangre de Drago" elaborada naturalmente y células madre vegetales de cebolla (Emolan®) y un grupo control "Vaselina". La toxicidad dérmica, se determinó con la técnica de Contero donde se administró una dosis única, por vía oral y dérmica, de 5000 mg/kg del extracto en ratas. Resultados: Mediante el análisis fitoquímico se determinó que es soluble en agua destilada y presenta metabolitos primarios y metabolitos secundarios. La crema a concentración del 10% obtuvo el (86%) de eficacia de cicatrización en comparación con la muestra de piel intacta; en la evaluación toxicológica, ninguna rata de cepa Holtzman presentó toxicidad dérmica. Conclusión: Se determinó que el extracto etanólico de los tubérculos de Ullucus tuberosus Caldas "olluco" si presenta actividad cicatrizante y no posee toxicidad dérmica.
Subject(s)
Animals , Mice , Rats , Wound Healing , Caryophyllales , Peru , Plants, Medicinal , Models, Animal , PhytochemicalsABSTRACT
Objective This study was designed to investigate the wound healing activity of aqueous extracts of Ullucus tuberosus (U. tuberosus) using in vitro models. Methods Lyophilized pulp and acetone extracts of U. tuberosus were produced using ultrasound extraction. The capacity for collagenase activation was evaluated using fluorescence detection of the enzymatic activity. Then, the influence of U. tuberosus extracts on cell proliferation, cell migration and synthesis of the extracellular matrix (ECM) proteins, metalloproteinase (MMP-1) and pro-collagen was analyzed using human dermal fibroblasts in culture. Results An increase in collagenase activity of 12% supports the utility of U. tuberosus as an agent for scar treatment. In addition, the extracts showed an increase in the proliferation and migration of human dermal fibroblasts and the production of pro-collagen and MMP-1 after treatment with U. tuberosus extracts. The increase in proliferation, migration and pro-collagen levels positively influenced the regeneration of scarless tissue during the proliferation phase, whereas the increase in MMP-1 may have favored the wound healing process during the remodeling and cellular differentiation phases. Conclusion The results of this study show for first time that U. tuberosus is a promising candidate to support scarless tissue regeneration.
ABSTRACT
Objective: This study was designed to investigate the wound healing activity of aqueous extracts of Ullucus tuberosus (U. tuberosus) using in vitro models. Methods: Lyophilized pulp and acetone extracts of U. tuberosus were produced using ultrasound extraction. The capacity for collagenase activation was evaluated using fluo-rescence detection of the enzymatic activity. Then, the influence of U. tuberosus extracts on cell proliferation, cell migration and synthesis of the extracellular matrix (ECM) proteins, metalloproteinase (MMP-1) and pro-collagen was analyzed using human dermal fibroblasts in culture. Results: An increase in collagenase activity of 12%supports the utility of U. tuberosus as an agent for scar treatment. In addition, the extracts showed an increase in the pro-liferation and migration of human dermal fibroblasts and the production of pro-collagen and MMP-1 after treatment with U. tuberosus extracts. The increase in proliferation, migration and pro-collagen levels positively influenced the regeneration of scarless tissue during the proliferation phase, whereas the increase in MMP-1 may have favored the wound healing process during the remodeling and cellular differentiation phases. Conclusion: The results of this study show for first time that U. tuberosus is a promising candidate to support scarless tissue regeneration.