ABSTRACT
Introducción: Vernonanthura patens (Kunth) H. Rob. es una especie que crece silvestre en el Ecuador, para la cual existen escasos antecedentes de estudios químicos y biológicos, y en ninguno de estos se aborda el estudio de fracciones lipídicas. Objetivos: estudiar las fracciones de compuestos lipídicos presentes en las hojas, tallos y flores de la especie. Métodos: la planta se recolectó en estado adulto, en época de floración, los órganos vegetales fueron secados en estufa por separado, se molinaron hasta tamaño de partícula 2 mm y se extrajeron por maceración con etanol. Los extractos etanólicos concentrados se separaron por columna cromatográfica, las fracciones lipídicas separadas con hexano fueron saponificadas y analizadas por el sistema acoplado de cromatografía gaseosa-espectrometría de masas. Resultados: se identificaron los ácidos grasos y compuestos insaponificables presentes en los órganos estudiados, encontrándose algunas diferencias de composición entre estos. Conclusiones: se identificaron 29 ácidos grasos y 8 compuestos insaponificables presentes en los órganos vegetales estudiados.
Introduction: Vernonanthura patens (Kunth) H. Rob. is a species that grows wild in Ecuador. Few chemical and biological studies have been conducted on this species, and none of them deal with lipid fractions. Objectives: study the fractions of lipid compounds present in the leaves, stems and flowers of the species. Methods: the plant was collected in its adult stage, during the flowering season. Plant organs were oven dried separately, ground to 2 mm particle size, and extracted by maceration with ethanol. The concentrated ethanolic extracts were separated by column chromatography. Lipid fractions were separated with hexane, and then saponified and analyzed by coupled gas chromatography mass spectrometry. Results: identification of the fatty acids and unsaponifiable compounds present in the organs studied revealed some differences in their composition. Conclusions: 29 fatty acids and 8 unsaponifiable compounds were identified in the plant organs studied.
ABSTRACT
Introducción: el D004, nuevo extracto lipídico purificado a partir de los frutos de la palma real cubana (Roystonea regia [Kunth] F. Cook), ha mostrado ser efectivo en modelos experimentales de hiperplasia prostática, y ha mostrado efectos antioxidantes y antiiflamatorios. Objetivos: identificar los alcoholes que pudieran estar presentes en el ingrediente activo D004 utilizando la cromatografía de gases acoplada a espectrometría de masa. Métodos: muestras de 9 lotes de D004 se sometieron a saponificación con una disolución de KOH/EtOH y las fracciones insaponificables fueron extraídas con n-hexano. Los alcoholes se analizaron como derivados trimetilsilil, obtenidos por reacción con N-metil, N-trimetilsilil trifluoroacetamida. La cuantificación se basó en el empleo de 1-eicosanol como patrón interno. Resultados: el D004 presenta un contenido total de alcoholes entre 0,18 y 5,10 por ciento. Esta fracción está compuesta principalmente por 1-octacosanol (52,94 por ciento), 1-triacontanol (25,91 por ciento) y 1-hexacosanol (14,40 por ciento); y presenta como componentes minoritarios: 1-docosanol, 1-tricosanol, 1-tetracosanol, 1-pentacosanol, 1-heptacosanol, 1-nonacosanol, 1-dotriacontanol, 1-tetratriacontanol, 1-tetradecanol, 1-pentadecanol, 1-hexadecanol, 1-octadecanol y 1-eicosanol, los seis últimos en niveles de trazas. Conclusiones: se identificaron y se cuantificaron mediante cromatografía de gases acoplada a espectrometría de masa los alcoholes grasos en el ingrediente activo D004. Los componentes mayoritarios fueron 1-octacosanol, 1-triacontanol y 1-hexacosanol
Introduction: D004, a new lipid extract purified from the Cuban royal palm (Roystonea regia [Kunth] F. Cook) fruits, has been shown to be effective in experimental models of prostate hyperplasia, and to exhibit antioxidant and anti-inflammatory effects. Objectives: to identify the fatty alcohols that could be present in D004 active ingredient using gas chromatography-mass spectrometry. Methods: samples of nine batches of D004 were submitted to saponification with KOH/EtOH solution and the unsaponifiable fractions were extracted with n-hexane. The alcohols were analyzed as trimetilsilil derivatives, which were obtained by reaction with MSTFA. The quantification was carried out by the internal standard method using 1-eicosanol. Results: the total content of alcohols in D004 ranged from 0,18 to 5,10 percent. This fraction was mainly composed of 1-octacosanol (52,94 percent), 1-triacontanol (25,91 percent), and 1-hexacosanol (14,40 percent); whereas the minority components were 1-docosanol, 1-tricosanol, 1-tetracosanol, 1-pentacosanol, 1-heptacosanol, 1-nonacosanol, 1-dotriacontanol 1-tetratriacontanol, 1-tetradecanol, 1-pentadecanol, 1-hexadecanol, 1-octadecanol and 1-eicosanol were minor components, being the last six alcohols present in trace levels. Conclusions: fatty alcohols were identified and quantified in D004 active ingredient by gas chromatography-mass spectrometry. The major components were 1-octacosanol, 1-triacontanol and 1-hexacosanol
Subject(s)
Fatty Alcohols , Gas Chromatography-Mass Spectrometry , Palm OilABSTRACT
Objective: To analyse the composition and content of the fatty acid and unsaponifiable matters in both tartary buckwheat seed oil and buckwheat seed oil. Methods: Fatty acids were obtained by the methods of KOH-EtOH(95%) saponification and treatment of inorganic acid. They were analyzed and determined by gas chromatography and GC/MS chromatography respectively. Results: 4-5 kinds of fatty acid were determined in both oils, and the content of unsaturated fatty acids in tatary buckwheat oil was 83.2% ( oleic acid 47.1%, linoleic acid 36.1%), buckwheat oil was 81.8% ( oleic acid 35.8% , linoleic acid 40.2% and linolenic acid 5.8%). The content of unsaponifiable matters in tartary buckwheat seed oil was 6.56%(?-sitosterol 54.37%), and in buckwheat seed oil was 21.90%(?-sitosterol 57.29%,?-tocopherol 1.41%). Conclusion: Both buckwheat oils were functional vegetable oils.