The inhibitory effects of N-myc down-stream regulated gene-2 on proliferation of bladder carcinoma cell line T24 in vitro / 中国医师杂志

Objective To explore the expression of a new candidate tumor suppressor N-myc downstream regulated gene 2 (NDRG2) in bladder cancer tissues and to investigate its clinical and pathological significance.Methods Formalin-fixed,paraffin-embedded tissue sections from 62 cases of bladder carcinomas and 10 cases of normal bladder tissues were analyzed retrospectively with immunohistochemistry (S-P method).Results The NDRG2 gene was highly expressed in normal bladder tissues,but low expressed in bladder carcinoma tissues.Positive expression of NDRG2 was detected in 8 of the 10 (80.0％) normal tissues and 40.3％ in bladder carcinoma ones (x2 =3.98,P ＜0.05).Furthermore,with the degree of malignancy increased,the positive expression of NDRG2 in bladder carcinoma samples was decreased.The expression of NDRG2 in bladder carcinoma was negatively correlated(r =-0.288,P ＜0.05) with C-myc(r =-0.436,P ＜0.01) and positively correlated with p53 in bladder carcinoma tissues(r =0.717,P ＜0.01).Conclusions The level of NDRG2 expression was lower in bladder carcinomas than in normal tissues.NDRG2 may play an important role in bladder carcinogenesis and in the progress of bladder cancers.

Molecular aspects of bladder cancer / Aspectos moleculares do câncer de bexiga

One of the most important objectives of genetic markers of cancer will be the possible identification of individuals at greatest risk in order to allow better management and prognosis. Many urological tumors were associated to various types of gene alterations with a great number of genes involved in the process, hindering gene therapy. This treatment uses specific techniques and one or several genes are manipulated in the laboratory in order to induce molecular alterations that may block the oncogenic process. The article addresses these issues emphasizing the importance of the new molecular biology techniques.

Um dos mais importantes objetivos dos marcadores genéticos para o câncer será a possível identificação do indivíduo com maior risco de doença, permitindo melhor conduta e prognóstico. Nos casos dos tumores urológicos, vários tumores têm sido relacionados a vários tipos de alterações genéticas, com um grande número de genes envolvidos, que dificulta muito a terapia gênica que é o processo no qual, por meio de técnicas específicas, gene ou genes são manipulados em laboratório para provocar alterações moleculares capazes de bloquear o processo oncogênico. O presente artigo aborda essas questões enfatizando a importância do advento das novas técnicas de biologia molecular.