ABSTRACT
The flower buds of Lonicera macranthoides (Shan Yin-Hua), represent an important traditional Chinese medicine and food ingredient. A phytochemical investigation of the 70% EtOH extract of the flower buds of L. macranthoides resulted in the isolation of 12 triterpenoids (1-12), including two new ursane-type nortriterpenes, 2α, 24-dihydroxy-23-nor-ursolic acid (1) and 2α, 4α-dihydroxy-23-nor-ursolic acid (2). Their structures were established by multiple spectroscopic methods and comparison with literature data. All isolated compounds were evaluated for their anti-inflammatory effects in LPS-activated RAW264.7 cells. Compounds 1 and 2 exhibited inhibitory effects on iNOS at the concentration of 30 μmol·L.
Subject(s)
Animals , Mice , Anti-Inflammatory Agents , Chemistry , Pharmacology , Drugs, Chinese Herbal , Chemistry , Enzyme Inhibitors , Chemistry , Pharmacology , Ethanol , Chemistry , Flowers , Chemistry , Lonicera , Chemistry , Macrophages , Metabolism , Molecular Structure , Nitric Oxide , Metabolism , Nitric Oxide Synthase Type II , Plant Extracts , Chemistry , Plants, Edible , Chemistry , Triterpenes , Chemistry , PharmacologyABSTRACT
ABSTRACT A new ursane-type triterpene ester, plectraterpene [3β-(decanoyloxy)-19-hydroxy-urs-12-ene] and four known steroidal compounds have been isolated from the aerial parts of Plectranthus montanus Benth. (syn. Plectranthus cylindraceus Hochst. ex Benth.), Lamiaceae. The known compounds were stigmasterol, sitosteryl ferulate, cholest-5-en-3-O-β-D-glucopyranoside and stigmasterol-3-O-β-D-glucopyranoside. Compounds plectraterpene, sitosteryl ferulate and stigmasterol-3-O-β-D-glucopyranoside are reported for the first time from this plant whereas compound cholest-5-en-3-O-β-D-glucopyranoside first time from the genus. The structures of these compounds were determined through spectral analysis, including extensive 2D NMR data as well as chemical methods and comparison with literature.
ABSTRACT
Objective: To study the chemical constituents of the acid hydrolysis products, in order to find some new aglycones from the hydrolysate of the saponin of Centella asiatica. Methods: The acid hydrolytic product was separated by repeated silica gel, Sephadex LH-20 chromatography. The cytotoxic activities in vitro of some compounds isolated were evaluated. Their structures isolated were identified by analysis of their spectral data of NMR, MS, IR, and UV. Results: Four aglycones, 2α,3β,23-trihydroxyurs-6,12-dien-28-oic acid (1), asiatic acid (2), 6β-hydroxy asiatic acid (3), terminolic acid (4), were isolated from the hydrolysis product. Conclusions: Compound 1, named centella-6-ene, was a new ursane-type aglycone and it showed no cytotoxic activities against the tested cancer cell lines.
ABSTRACT
A new ursane-type triterpenoid saponin, 2α,3α,24-trihydroxyurs-12,20(30)-dien-28-oic acid β-D-glucopyranosyl ester (1), together with six known triterpenoid saponins, was isolated and characterized from the aerial parts of Clematoclethra scandens subsp. actinidioides.