ABSTRACT
Objective To explore the clinical value of DNA quantitative analysis combined with colposcopy in the diagnosis of cervical cancer and precancerous lesions.Methods From January 2015 to January 2017, 500 women with sexual history were enrolled for obstetrics and gynecology.The sensitivity, specificity, and accuracy of DNA quantitative analysis, colposcopy, and the combination of them were evaluated by the pathological examination.Results The DNA quantitative analysis showed that the sensitivity of cervical lesions was 73.75％, the specificity was 94.05％,the positive predictive value was70.24％, and the accuracy rate was 90.8％.Colposcopy showed that the sensitivity of cervical lesions was 82.5％, specificity was 91.43％, positive predictive value was 64.71％, and the accuracy rate was 90.0％.When the two were used together, the sensitivity was 87.5％, the specificity was 95.71％, and the highest accuracy was 94.4％.When three methods were compared together, the differences among the specificity and the accuracy were statistically significant (P ＜ 0.05).For any pairwise comparison, the accuracy of the combined examination was better than that of DNA quantitative analysis and colposcopy (P ＜ 0.05).The specificity of combined examination was significantly better than that of colposcopy (P ＜ 0.05).The sensitivity of combined examination was better than that of DNA quantitative analysis (P ＜ 0.05).Conclusions DNA quantitative analysis combined with colposcopy screening has a higher sensitivity, specificity and accuracy, and it has the best diagnostic value for screening of cervical cancer and precancerous lesions.
ABSTRACT
Objective To investigate the application value of combination of thinprep cytologic test (TCT),human papilloma virus (HPV),c-MYC and human chromosome telomerase gene (hTERC) genes to screen cervical cancer.Methods A total of 230 cases of the study objects was detected with TCT and HP,respectively.Amplification of c-MYC and hTERC genes was tested with fluorescence in situ hybridization (FISH) method.The histopathological results were the gold standard,and the sensitivity,specificity and accuracy of cervical intraepithelial neoplasia (CIN) Ⅱ / Ⅲ and squamous cell carcinoma (SCC) were compared with the four combined detection.Results Of 230 screened patients,there were 124 cases of TCT positive,155 cases of HPV positive,c-MYC gene amplified in 118 cases,and hTERC gene amplified in 128 cases.When TCT,HPV,c-MYC,and hTERC were used alone,the highest sensitivity was HPV (84.5％),the highest specificity was c-MYC (97.6％),and the highest accuracy was hTERC (85.2％).When the three indexes were used in coordination with each other,the sensitivity and accuracy of TCT + HPV + hTERC were the highest (98.6％ and 90.9％),and the specificity of TCT + c-MYC + hTERC was the highest (79.3％).When the four indexes were used together,the sensitivity was 98.6％,the specificity was 72.0％,and the accuracy was 89.1％.Conclusions Combined examination can improve the sensitivity and accuracy of screening cervical lesions,and the three combination of TCT + HPV + hTERC had the best effect,and c-MYC gene detection had the highest specificity.
ABSTRACT
Objective To investigate the application value of combination of thinprep cytologic test (TCT),human papilloma virus (HPV),c-MYC and human chromosome telomerase gene (hTERC) genes to screen cervical cancer.Methods A total of 230 cases of the study objects was detected with TCT and HP,respectively.Amplification of c-MYC and hTERC genes was tested with fluorescence in situ hybridization (FISH) method.The histopathological results were the gold standard,and the sensitivity,specificity and accuracy of cervical intraepithelial neoplasia (CIN) Ⅱ / Ⅲ and squamous cell carcinoma (SCC) were compared with the four combined detection.Results Of 230 screened patients,there were 124 cases of TCT positive,155 cases of HPV positive,c-MYC gene amplified in 118 cases,and hTERC gene amplified in 128 cases.When TCT,HPV,c-MYC,and hTERC were used alone,the highest sensitivity was HPV (84.5％),the highest specificity was c-MYC (97.6％),and the highest accuracy was hTERC (85.2％).When the three indexes were used in coordination with each other,the sensitivity and accuracy of TCT + HPV + hTERC were the highest (98.6％ and 90.9％),and the specificity of TCT + c-MYC + hTERC was the highest (79.3％).When the four indexes were used together,the sensitivity was 98.6％,the specificity was 72.0％,and the accuracy was 89.1％.Conclusions Combined examination can improve the sensitivity and accuracy of screening cervical lesions,and the three combination of TCT + HPV + hTERC had the best effect,and c-MYC gene detection had the highest specificity.