ABSTRACT
Objective The purpose of this study was to develop the mechanism of vacuolar H+-ATPase regulation of vaginal microenvironment.Methods In this research,53 women were divided into three groups.Their age,serum estradiol (E2),serum follicle stimulating hormone (FSH),vaginal pH value,and mRNA expression of vacuolar H +-ATPase (VHA) on the ectocervical-vaginal epithelial cells were analyzed.Results (1) As serum E2 levels decreased,the vaginal pH values increased and VHA mRNA declined (P ＜ 0.01).(2) Immunohistochemistry scores decreased in the three groups.VHA expression decreased in human ectocervical-vaginal epithelial tissues except basal cells.(3) The expression of VHA was positive correlated with estradiol,while negative correlated with age and vaginal pH value (P ＜ 0.01).Conclusions Estradiol could regulate the genetic transcription and synthetic of VHA protein under vaginal microenvironment.