ABSTRACT
Valeriana amurensis Smir. ex Kom. widely distributed in the northeast region of China and some region in Russia and Korea, and its underground parts (roots and rhizomes) being used to cure nervous system diseases such as insomnia. The active components including the essential oil and iridoids of underground parts were investigated in different harvest periods in order to evaluate the quality for the roots and rhizomes of V. amurensis. The content of the essential oil was obtained by hydrodistillation and bornyl acetate in the oil was quantitated by GC-EI. The iridoids, valepotriates were determined by potentiometric titration and the main component, valtrate was quantitated by HPLC-UV. The factors of biomass were considered in the determination of collection period. Statistical analysis of results showed that, the highest content of the essential oil per plant was 22.69 µl in withering period and then 21.58 µl in fruit ripening period, while the highest contents of bornyl acetate, valepotriates and valtrate per plant were 2.82 mg, 31.90 mg and 0.98 mg in fruit ripening period separately. Fruit ripening period was decided as the best harvest period for the content of active constituents and output of drug, and it would provide scientific basis for the artificial cultivation of V. amurensis.
Valeriana amurensis Smir. ex Kom. Se distribuye ampliamente en la regioÌn noreste de China y en algunas regiones de Rusia y Corea, y sus partes subterraÌneas (raiÌces y rizomas) se utilizan para curar enfermedades del sistema nervioso como el insomnio. Se investigaron los componentes activos, incluidos el aceite esencial y los iridoides de las partes subterraÌneas de V. amurensis en diferentes periÌodos de cosecha para evaluar la calidad de las raiÌces y rizomas. El contenido del aceite esencial se obtuvo mediante hidrodestilacioÌn y el acetato de bornilo en el aceite se cuantificoÌ por GC-EI. Los iridoides, valepotriatos se determinaron mediante valoracioÌn potenciomeÌtrica y el componente principal, el valtrato se cuantificoÌ por HPLC-UV. Los factores de biomasa fueron considerados en la determinacioÌn del periÌodo de recoleccioÌn. El anaÌlisis estadiÌstico de los resultados mostroÌ que el mayor contenido de aceite esencial por planta fue de 22,69 µl en el periÌodo de marchitacioÌn y luego de 21,58 µl en el periÌodo de maduracioÌn de la fruta, mientras que el mayor contenido de acetato de bornilo, valepotriatos y valtrato por planta fue de 2.82 mg, 31.90 mg y 0,98 mg, respectivamente, en el periÌodo de maduracioÌn de la fruta por separado. Se definioÌ el periÌodo de maduracioÌn de la fruta como el mejor periÌodo de cosecha para el contenido de constituyentes activos y la produccioÌn de droga, lo cual proporcionariÌa una base cientiÌfica para el cultivo artificial de V. amurensis.
Subject(s)
Valerian/chemistry , Oils, Volatile/chemistry , Plant Roots/chemistry , Seasons , Camphanes/analysis , Chromatography, High Pressure Liquid , Spectrometry, Mass, Electrospray Ionization , Rhizome/chemistry , Iridoids/analysisABSTRACT
Abstract Previous pre-clinical studies demonstrated that a valepotriates enriched fraction from Valeriana glechomifolia F.G. Mey., Caprifoliaceae, was effective against lipopolysaccharide from Escherichia coli (LPS)-induced sickness behavior as well as significantly decreased the cortical expression of pro inflammatory cytokines interleukin-1β and tumor necrosis factor-α. Other studies revealed anti-inflammatory properties of V. wallichii and V. amurensis. These findings open up new perspectives for Valeriana genus pharmacology, once it has been commonly associated to sedative and anxiolytic properties. The aim of this study was to investigate the antichemotactic, antinociptive and anti-inflammatory activities of a valepotriate-enriched fraction obtained from aerial and subterranean parts of V. glechomifolia submitted to supercritical CO2 extraction. The biological activities were assessed by means of formalin test in CF1 mice and Wistar rat's leukocytes migration assay (modified Boyden chamber method). Valepotriate-enriched fraction (1, 10 and 30 mg/kg, p.o.) inhibited the nociceptive behavior in the late phase of the formalin test in a dose dependent manner. The effect of the valepotriate-enriched fraction highest dose was comparable with that of diclofenac 50 mg/kg (p.o.). Valepotriate-enriched fraction (0.1-1 µg/ml) inhibited the leukocyte migration induced by lipopolysaccharide from Escherichia coli in a concentration dependent manner. This antichemotatic effect was comparable with that of indomethacin (0.1-1 µg/ml) and better than diclofenac (1 µg/ml) effect. This study demonstrated for the first time that a valepotriate-enriched fraction obtained from V. glechomifolia display a peripheral anti-inflammatory like activity.