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OBJECTIVE@#To investigate the effect of JAG1 on the malignant phenotype of triple-negative breast cancer (TNBC) and its role in angiogenesis in breast cancer microenvironment.@*METHODS@#The expressions of Notch molecules were detected in human TNBC 231 and 231B cells using RT-qPCR. Five female nude mice were inoculated with 231 cells and another 5 with 231B cells into the mammary fat pads, and 4-6 weeks later, the tumors were collected for immunohistochemical and immunofluorescence tests. 231 cells and 231B cells were treated with recombinant JAG (rJAG) protein and DAPT, respectively, and changes in their malignant phenotypes were assessed using CCK-8 assay, Hoechst 33258 staining, wound healing assay, Transwell chamber assay and endothelial cell adhesion assay. Western blotting was used to detect the changes in the expressions of proteins related with the malignant phenotypes of 231 and 231B cells. The effects of conditioned medium (CM) derived from untreated 231 and 231 B cells, rJAG1-treated 231 cells and DAPT-treated 231B cells on proliferation and tube formation ability of cultured human umbilical vein endothelial cells (HUVECs) were evaluated using CCK-8 assay and tube-forming assay.@*RESULTS@#The expression of JAG1 was higher in 231B cells than in 231 cells (P < 0.05). Tumor 231B showed higher expression of VEGFA and CD31. Compared with 231-Blank group, the migration, invasion and adhesion of 231 cells in 231-rJAG1 were significantly enhanced (P < 0.05). Protein levels of Twist1 and Snail increased (P < 0.01), anti-apoptotic protein Bcl-2 increased (P < 0.05), while DAPT inhibited the related phenomena and indicators of 231B. The 231-rJAG1-CM increased the cell number and tubule number of HUVEC (P < 0.05).@*CONCLUSION@#JAG1 may affect the malignant phenotype of TNBC and promote angiogenesis in the tumor microenvironment.
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Animals , Female , Humans , Mice , Cell Line, Tumor , Cell Movement , Cell Proliferation , Culture Media, Conditioned , Human Umbilical Vein Endothelial Cells/metabolism , Jagged-1 Protein/metabolism , Mice, Nude , Neovascularization, Pathologic/metabolism , Platelet Aggregation Inhibitors , Sincalide/metabolism , Triple Negative Breast Neoplasms/metabolism , Tumor MicroenvironmentABSTRACT
Objective: To investigate the expression of vascular endothelial cell growth factor (VEGF) in the immediate replantation of pulp healing in the rats, and to clarify the effect and its mechanism of erythropoietin (EPO) on immediate pulp reconstruction in the rats. Methods: Eighty 4-week-old healthy male Wistar rats were randomly divided into non-tooth extraction group, negative control (normal saline) group, positive control (gentamicin) group and EPO group; there were twenty rats in each group. The teeth in each group were immersed in its corresponding solution for 4 min before replantation. Four rats were killed on the days 3, 7, 14, 21 and 28, respectively, and the specimens were made in the operation area. HE staining was used to observe the pulp revascularization in different time periods. Immunohistochemical staining was used to observe the protein expression levels of VEGF in odontal tissue of the rats in each group in different time periods. Results: The HE staining results showed that compared with non-tooth extraction group, the pulp tissue of replanted teeth of the rats in normal saline group had more inflammation, less root development, less restorative dentin and cementum deposition, and wider apical pores; the inflammation of pulp tissue of replanted teeth of the rats in gentamicin group and EPO group was mild, and the root development was relatively good; there were more deposits of restorative dentin and cementum, and the apical pores were narrowed. The immunohistochemical results showed that compared with non-tooth extraction group, the positive expressions of VEGF in odontal tissue of the rats at the days 3, 7 and 14 in the other groups were strong. Afterwards, the positive expression levels of VEGF were decreased gradually with the prolongation of time. The average optical density (AOD) of VEGF positive area indicated that EPO group > gentamycin group > normal saline group > non-tooth extraction group. Compared with non-tooth extraction group, the protein expression levels of VEGF in odontal tissue of the rats in normal saline group, gentamicin group and EPO group at 3, 7, 14 and 21 d after operation were significanty increased (P0.05). Compared with normal saline group, the protein expression levels of VEGF in odontal tissue of the rats in gentamicin group and EPO group at 3, 7, 14 and 21 d after operation were significantly increased (P0.05). Compared with gentamicin group, the protein expression levels of VEGF in odontal tissue of the rats in EPO group at every time points had no significant differences (P>0.05). Conclusion: EPO can increase the expression of VEGF, induce angiogenesis in pulp tissue, and provide the rich vascular bed for replantated teeth, so as to exert the potential of dental pulp defense and repair, and promote the healing of replanted teeth.
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AIM: To study the preventive effect of Qilin pill on ovarian hyperstimulation syndrome (OHSS) after in vitro fertilization and embryo transfer (IVF-ET) and its effects on vascular endothelial growth factor (VEGF), tissue factor (TF) and tissue factor pathway inhibitor (TFPI) in plasma. METHODS: Sixty-four patients undergoing IVF-ET treated in our hospital from January 2016 to January 2019 were selected. On the day of ovulation induction injection of human chorionic gonadotropin (HCG), 32 patients with high risk factors of OHSS were randomly divided into two groups. The control group received western medicine therapy, while the observation group received extra Qilin pill. The incidence of mild to moderate OHSS, fresh cycle transplant cancellation rate, plasma VEGF, TF, TFPI levels, and clinical outcomes of patients undergoing IVF-ET (HCG positive rate, biochemical pregnancy rate, clinical pregnancy rate) were compared between the two groups.RESULTS:There was no severe OHSS occurred in the two groups, the incidence of OHSS in the observation group (12.50%) and the cancellation rate of fresh cycle transplantation (15.63%) were lower than those in the control group (50.00%, 43.75%)(χ2=6.063,P=0.014); The levels of VEGF and TF in the observation group on the day of egg retrieval and embryo transfer were [(368±103) pg/mL, (392±91) pg/mL],[(24±4)pg/ mL,(29±4) pg/mL], which were lower than the control group [(436±117) pg/mL, (448±108) pg/mL],[(26±4) pg/mL, (31±4) pg/mL] (t=2.450,2.237,4.093,5.204,P=0.017,0.029,<0.001,<0.001); The plasma TFPI levels in the observation group on the day of egg retrieval and embryo transfer were [(73±18) ng/mL,(66±12) ng/mL], higher than the control group [(62±16)ng/mL, (58±10) ng/mL](t=2.550,3.032,P=0.014,0.004); The biochemical pregnancy rate in the observation group (8.70%) was lower than that in the control group (42.86%) (χ2=4.147, P=0.042),the clinical pregnancy rate (91.30%) was higher than that of the control group (57.14%) (χ2=4.147,P=0.042).CONCLUSION:Qilin pill can prevent the occurrence of severe OHSS after IVF-ET, reduce the occurrence of mild to moderate OHSS, decrease the cancellation rate of fresh cycle transplantation and improve the pregnancy outcome after IVF-ET; Its mechanism may be related to the regulation of the expression of VEGF, TF and TFPI.
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Objective To study the expression of vascular endothelial cell growth factor (VEGF) and its receptor 2 (VEGFR2) and apoptosis in the epididymis of rats with chronic arsenic poisoning.Methods Forty male Sprague-Dawley rats,weighting 160-200 g,were selected and randomly divided into four groups:high dose [60.0 mg/L sodium arsenite (NaAsO2)],medium dose (12.0 mg/L NaAsO2),low dose arsenic infected groups (2.4 mg/L NaAsO2),and control group (distilled water).The rats were treated with arsenic through drinking water for 6 consecutive months.At the end of the experiment,all rats were killed and epididymis was separated rapidly,apoptosis cells of rat epididymis were determined via the TUNEL method,the expressions of VEGF and VEGFR2 were observed through immunohistochemistry.Results The apoptosis of the epididymis tubules epithelial cells were compared in control,low,medium,and high dose groups (124.68 ± 6.59,138.96 ± 8.48,152.59 ± 10.79,170.69 ±16.60),the differences were statistically significant (F =10.562,P ＜ 0.05),with increased exposure doses of arsenic,apoptosis cells were increased.VEGF expression levels of the body,head and tail in epididymis were compared in control,low,medium,and high dose groups (148.50 ± 0.60,134.20 ± 0.85,98.23 ± 0.80;136.70 ± 0.95,128.20 ±0.72,90.30 ± 0.12;127.80 ± 0.62,117.60 ± 0.72,84.51 ± 0.60;120.26 ± 0.46,90.12 ± 0.36,66.43 ± 0.90),the differences were statistically significant (F =46.445,45.867,41.381,P ＜ 0.05),and VEGF expression levels in arsenic infected groups were lower than that in control group (P ＜ 0.05);VEGFR2 expression levels of the head and tail (130.70 ± 0.89,128.80 ± 0.70;126.30 ± 0.65,121.42 ± 0.62;120.26 ± 0.12,117.84 ± 0.55;102.60 ±0.78,104.92 ± 1.15) were compared,the differences were statistically significant (F =3.452,2.701,P ＜ 0.05),and VEGFR2 expression levels in arsenic infected groups were lower than that in control group (P ＜ 0.05).Conclusion Arsenism has inhibitory effect on the expression of VEGF and VEGFR2 in the epididymis,leading to epididymis epithelial cell apoptosis,which indicates it may play an important role in male infertility.
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Objective: To observe the therapeutical effect of Yishen Juanbi Pill (YJP) on type II collagen induced arthritis (CIA) in rats and the histopathologic changes in ankle joint and to explore its possible mechanism on rheumatoid arthritis (RA). Methods: The therapeutical effect of YJP on CIA rat paw swelling and the histopathologic morphology of the local tissue of ankle joint were observed by the type II CIA rat model. The expression of VEGF of the synovial membrane was assayed by the immunohistochemical method. The changes in VEGF gene expression of peripheral lymphocytes and hepatic tissue were analyzed with gene chip after the treatment with YJP. Results: The decreased swelling degrees of foot of rats in low-, mid-, and high-dose YJP groups were increased obviously than that of the model group (P 1.5). Conclusion: YJP can significantly inhibit joint swelling. YJP can significantly down-regulate the VEGF-B gene expression, thereby inhibit the formation of synovial tissue pannus and reduce cartilage and bone damages.
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Objective To explore the evaluation of tumor specific growth factor (TSGF) ,alpha‐fetoprotein (AFP) and vascular endothelial cell growth factor(VEGF) levels in therapeutic effect of primary hepatic carcinoma (PHC) .Methods Serum TSGF , AFP and VEGF levels were detected on 1 d before treatment ,4 ,28 d after treatment in 75 patients with PHC .The relation between serum VEGF with AFP on 1 d before treatment was analyzed .Results The levels of serum TSGF ,AFP and VEGF on 4 ,28 d after treatment in 75 cases of PHC were significantly lower than those on 1 d before treatment ,the difference was statistically significant (P 0 .05) .Conclusion TSGF ,AFP and VEGF can serve as the indicators for evaluating PHC prognosis .
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We built rats model of endometriosis applying autologus endometrial transplants to compare the graft vol-ume of the modeling rats before and after dosing,serum tumor necrosis factor alpha(TNF-α)levels in rats,and the expressions of vascular endothelial cell growth factor(VEGF)in ectopic endometrium.To compare with the control group,the graft volume of DingKunDan group was reduced and the serum TNF-αlevel declined ,and also the ex-pression of VEGF in ectopic endometrium was reduced.Their differences were statistically significant (P <0.05). DingKunDan can inhibit the growth of ectopic endometrium in endometrosis model rats.The mechanism may be re-lated to the decrease of the serum TNF-αlevel and the VEGF expression.
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ObjectiveTo observe the effect of different extracts ofTangwang Mingmu Granule on hypoxia induced gene expressions in vascular endothelial cells.Methods COCl2 intervention cells were used to copy hypoxia models. Human umbilical vein endothelial cells EA. Hy926 were divided into blank group, hypoxia model group,Tangwang Mingmu Granule group, extract 1 (glycosides and flavonoids) group, extract 2 (orgain acids and polysaccharides) group and extract 3 (alkaloids) group. The changes in gene expressions of VEGF, VEGFR-1, VEGFR-2, ICAM-1 and IL-1α mRNA were detected by semi-quantitative RT-PCR.ResultsThe gene expression levels of VEGF, VEGFR-1, VEGFR-2, ICAM-1 and IL-1α were significantly up-regulated under the hypoxic condition (Palkaloids>organic acids and polysaccharides inTangwang Mingmu Granule.
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BACKGROUND: Vascular endothelial growth factor (VEGF) is involved in the growth of new blood vessels that feed tumors and kinesin spindle protein (KSP) plays a critical role in mitosis involving in cell proliferation. Simultaneous silencing of VEGF and KSP, an attractive and viable approach in cancer, leads on restricting cancer progression. The purpose of this study is to examine the therapeutic potential of dual gene targeted siRNA cocktail on human hepatocellular carcinoma Hep3B cells. RESULTS: The predesigned siRNAs could inhibit VEGF and KSP at mRNA level. siRNA cocktail showed a further downregulation on KSP mRNA and protein levels compared to KSP-siRNA or VEGF-siRNA, but not on VEGF expression. It also exhibited greater suppression on cell proliferation as well as cell migration or invasion capabilities and induction of apoptosis in Hep3B cells than single siRNA simultaneously. This could be explained by the significant downregulation of Cyclin D1, Bcl-2 and Survivin. However, no sigificant difference in the mRNA and protein levels of ANG2, involving inhibition of angiogenesis was found in HUVECs cultured with supernatant of Hep3B cells treated with siRNA cocktail, compared to that of VEGF-siRNA. CONCLUSION: Silencing of VEGF and KSP plays a key role in inhibiting cell proliferation, migration, invasion and inducing apoptosis of Hep3B cells. Simultaneous silencing of VEGF and KSP using siRNA cocktail yields promising results for eradicating hepatocellular carcinoma cells, a new direction for liver cancer treatment.
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Humans , Kinesins/genetics , Apoptosis/genetics , Gene Silencing , RNA, Small Interfering/genetics , Vascular Endothelial Growth Factor A/genetics , Cell Proliferation/genetics , Tetrazolium Salts , Transfection , Cysteine Proteinase Inhibitors/metabolism , Down-Regulation , Cell Movement , Blotting, Western , Kinesins/metabolism , Annexin A5 , Genes, bcl-2 , Cyclin D1/metabolism , Vesicular Transport Proteins/metabolism , Cell Line, Tumor , Vascular Endothelial Growth Factor A/metabolism , Inhibitor of Apoptosis Proteins/metabolism , Human Umbilical Vein Endothelial Cells/metabolism , Real-Time Polymerase Chain Reaction , Flow Cytometry , Survivin , Mitosis/geneticsABSTRACT
To investigate the expression of vascular endothelial cell growth factor (VEGF) and its receptors in vitreous of patients with diabetic retinopathy ( DR), and to discuss its role in the development of DR. ●METHODS: Selected 13 patients (16 eyes) with DR and 15 healthy people (15 eyes), the expression of VEGF and its receptors (fms-like tyrosine kinase-1, Flt-1 and kinase insert domain containing receptor, KDR) were evaluated by immunohistochemistry in vitreous. The levels of VEGF, the Flt-1 and KDR in vitreous of patients with DR were examined with enzyme linked immunosorbent assay (ELlSA). ● RESULTS: lmmunohistochemical staining showed that the expression of VEGF, Flt-1 and KDR in vitreous vessel membrane of patients with DR was increased significantly. And the levels of VEGF, Flt - 1 and KDR in vitreous of patients with DR were obviously higher than that in the control group (P ● CONCLUSlON: VEGF, Flt - 1 and KDR were widely expressed in vitreous of patients with DR, and were positively related to micro-angiogenesis of DR patients. lt proved that VEGF and its receptors played important roles in the occurrence and development of DR.
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Objective To investigate the molecular mechanism of epithelial-mesenchymal transition (EMT) induced by activated vascular endothelial growth factor receptor-1 ( VEGFR-I ) in cell line MHCC97-H.Methods MHCC97-H cells were cultured in DMEM with 1％ fetal bovine serum (control group),10 μmol/L PP2 (PP2 group),10 μmol/L PBS (PBS group),50 μmol/L VEGF-B (VEGF-B group),l0μmol/L PP2 and 50 μmol/L VEGF-B (PP2 +VEGF group),10 μmol/L PBS and 50 μmol/L VEGF-B (PBS + VEGF-B group),respectively.Protein expressions of epithelial marker E-cadherin,α-catenin and mesenchymal marker vimentin and N-cadherin were detected by Western blot.The expression sites of E-cadherin,α-catenin and mesenchymal marker vimentin and N-cadherin were detected by cell immunofluorescence.The ability of invasion and migration of cell line MHCC97-H were assessed by cell invasion and migration test.All data were analyzed by the t test.Results The expressions of E-cadherin,α-catenin,vimentin and N-cadherin were 3.23 +0.76,3.01 ±0.25,3.01 +0.22 and 2.63 +0.40 in the control group,4.18 +0.32,3.29 +0.11,4.85 +0.36 and 3.02 +0.52 in the PP2 group,2.83 +0.65,3.03 +0.27,1.37 ±0.24 and 2.98 ±0.36 in the PBS group,2.06 ±0.15,2.84 ±0.76,5.79 ± 0.38 and 5.54 ± 0.28 in the VEGF-B group,6.12 ± 0.08,5.45 ± 0.37,3.36 ± 0.42 and 3.26 ±0.13 in the PP2 + VEGF-B group and 1.36 ±0.54,1.26 ±0.45,4.05 ±0.17 and 1.05 ±0.33 in the PBS +VEGF-B group.There was a significant difference in the expressions of E-cadherin and α-catenin between the PP2 +VEGF-B group and the VEGF-B group (t =7.625,9.931,P ＜ 0.05 ).The expressions of vimentin and N-cadherin in the PP2 + VEGF-B group were significantly lower than those in the VEGF-B group (t =12.001,11.910,P ＜ 0.05).Six hours after the treatment with VEGF-B,the numbers of MHCC97-H migrated were 19 ± 1,5 ± 2and 16 ± 1 in the VEGF-B group,PP2 + VEGF-B group and PBS + VEGF-B group,respectively.The number of MHCC97-H cells migrated in the VEGF-B group was greater than that in the PP2 ± VEGF-B group ( t =13.566,P ＜ 0.05 ).The number of MHCC97-H cells passed through the Boyden chamber was 4 + 2,which was significantly less than 16 ± 1 of the VEGF-B group (t =12.350,P ＜0.05).Conclusion EMT induced by activated VEGFR-1 was mediated via c-Src kinase signal transduction in MHCC91-H cell line,and c-Src may be a potential target to interfere the invasion and migration of hepatic cancer cells.
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Objective To discuss the effects of bone marrow-derived mesenchymal stem cells (MSCs) on vascular endothelial growth factor (VEGF) in lung tissue and in plasma, and extra-vascular lung water at the early stage of smog inhalation injury. Method The rabbit model of smog inhalation injury was established by using home-made smog generator, and the rabbit models were randomly(random number) divided into control group (group S, n = 32) and MSCs treatment group (group M, n = 32). Ten mL phosphate-buffered saline (PBS) was injected into ear marginal vein immediately after injury in rabbits of group S. The third generation of MSCs 1/10/10 mL PBS was injected into ear marginal vein immediately after injury in rabbits of group M. The levels of VEGF in peripheral blood and lung tissue were neasured 0 h,2 h,4 h and 6 hours after injection respectively, and analyzed. The right lung of rabbits was taken out to measure and calculate lung water mass fraction after experiment.Results In lung tissue, the levels of VEGF decreased gradually in group S (P ＜ 0.05), and though the levels of VEGF in lung tissue appeared with significant decreasing trend in group M (P ＜ 0.05), they were still higher than those of group S at corresponding intervals ( P ＜ 0.05). In peripheral blood, the levels of VEGF increased gradually in group S ( P ＜ 0. 05), and the levels of VEGF in group M appeared with markedly increasing trend ( P ＜ 0.05),but they were lower significantly than those in group S at corresponding intervals ( P ＜ 0.05). Conclusions MSCs engraftment to the rabbits with smog inhalation injury could increase VEGF in lung tissue, decrease VEGF in plasma and reduce extra-vascular lung water, showing protective effect on smog inhalation injury to a certain extent.
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BACKGROUND: This study was undertaken to determine the effect of mesenchymal stem cells (MSCs) engraftment on vascular endothelial cell growth factor (VEGF) in lung tissue, plasma and extravascular lung water at early stage of smoke inhalation injury. METHODS: A rabbit smoke inhalation injury model was established using a home-made smoke inhalation injury generator, and rabbits were divided into two groups randomly: a control group (S group, n=32) and a MSCs treatment group (M group,n=32). 10 ml PBS was injected via the ear marginal vein immediately at injury into the S group. Third generation MSCs with a concentration of 1×107/10 ml PBS were injected via the ear marginal vein immediately at injury into the M group. VEGF in peripheral blood and lung tissue were measured at 0 (baseline), 2, 4 and 6 hours after injection respectively and analyzed. The right lungs of rabbits were taken to measure lung water mass fraction. RESULTS: In the lung tissue, VEGF decreased gradually in the S group (P<0.05) and signifi cantly decreased in the M group (P<0.05), but it increased more signifi cantly than the values at the corresponding time points (P<0.05). In peripheral blood, VEGF increased gradually in the S group (P<0.05) and markedly increased in the M group (P<0.05), but it decreased more signifi cantly than the values at corresponding time points (P<0.05). CONCLUSION: MSCs engraftment to smoke inhalation injury could increase VEGF in lung tissue, decrease VEGF in plasma and reduce extravascular lung water, indicating its protective effect on smoke inhalation injury.
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point (P<0.05).Conclusion Transplantation of MBMCs promotes the expression of VEGF, up-regulates the MVD value in the acute injury livers, and facilitates the recovery of liver function.
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Objective To investigate the influence of gene transfection antiangiogenesis on microvessel and relative cytokine of hypertrophic scar of rabbits' ear.Methods The hypertrophic scar of rabbtis' ear was reproduced.On the 10th day after epithelization,Ad-METH-1 was injected into tissue of scar.30 days later,the microvessel of scar-tissue was detected by microcirculation microscope.Meanwhile.H&E and immunohistochemical stains were performed.Then the results were analyzed.Results 30 days after Ad-METH-1 injection.in experimental groups,the microvascular count of scar tissue was 12.38±2.56,the percentage of VEGF positive cells was 17.64%,and the percentage of bFGF positive cell was 18.24%:while in the control groups,the microvascular count of scar tissue was 48.12±6.46.the percentage of VEGF positive cell was 31.34%.and the percentage of bFGF positive eell was 28.26%.Results revealed that the count of microvessel of scar tissue in the experimental groups was lower than that in the control groups,between which there was the difference in statistics(P＜0.01).and that the percentage of VEGF and bFGF positive cells of scar tissue in the expenmental groups were lower than that in the control groups.between which there was the difference in statistics(P＜0.05).Conclusion Ad-METH-1 has marked inhibitory effects on scar tissue hyperplasia of rabbits' ear,angiogenesis and expression of VEGF and bFGF.Using antiangiogenesis therapy at the early phase could inhibit the formation of hypertrophic scar.Gene transfection antiangiogenesis therapy could bid fair to become an effective method to prevent and treat hypertrophic scar.
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OBJECTIVE: Endometriosis is the presence of normal endometrial mucosa (glands and stroma) abnormally implanted on the outside of uterus. The pathophysiology of endometriosis is not clear yet, but Sampson's theory of the transplantation of endometrial tissue onto the pelvic peritoneum via retrograde menstruation is most widely accepted. Vascular endothelial cell growth factor (VEGF) is involved in the pathophysiology of endometriosis via its angiogenetic property. This study was designed to investigate whether single nucleotide polymorphism and its haplotype and diplotype of VEGF genes are associated with the risk of advanced endometriosis or not. METHODS: This study investigated 260 patients of endometriosis; they underwent operation, and were diagnosed with endometriosis stage III, IV histopathologically. And control group of 199 women underwent surgery with benign ovarian cyst. The single nucleotide polymorphisms of VEGF gene were -2578C>A, 405G>C, 936C>T. They were analyzed by polymerase chain reaction and restriction fragment length polymorphism, and haplotype and diplotype analysis were done. RESULTS: The result of this study showed no association among -2578C/A, 405G>C, 936C>T single nucleotide polymorphisms and severe endometriosis. Haplotype and diplotype of -2578C>A, 405G>C, 936C>T in the VEGF gene were shown to have no association with endometriosis. We found no association between VEGF genetic polymorphism and risk of endometriosis. And haplotype and diplotype analysis also revealed no statistically significant value between VEGF polymorphism and endometriosis. CONCLUSIONS: So, the results of this study suggest polymorphism of VEGF gene may not be associated with risk of endometriosis in Korean women.
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Female , Humans , Endometriosis , Endothelial Cells , Haplotypes , Menstruation Disturbances , Mucous Membrane , Ovarian Cysts , Peritoneum , Polymerase Chain Reaction , Polymorphism, Genetic , Polymorphism, Restriction Fragment Length , Polymorphism, Single Nucleotide , Transplants , Uterus , Vascular Endothelial Growth Factor AABSTRACT
Objective To investigate the role of vascular endothelial growth factor-C (VEGF-C) and its receptors in the formation of lymphatic vessels and lymphatic metastasis in gastric cancer. Methods By the domestic and overseas literatures review,the expressions of VEGF-C and its receptors in gastric cancer,their role in tumor lymphatic metastasis and prospect in treatment of gastric cancer were summarized. Results There was a significant correlation between VEGF-C and its receptors and the formation of lymphatic vessels and lymphatic metastasis in gastric cancer. VEGF-C high expression might be an early event in lymphatic metastasis and could be considered as an independent predictive factor of lymphaticmicrometastasis. By inhibition of gastric cancer cell from secrete VEGF-C or blockage of the interaction of VEGF-C with VEGFR-3,it was possible to inhibit tumor angiogenesis and the invasion and distant spread of cancer cells,thereby decreased mortality and improve survival. Conclusion VEGF-C and its receptors may promote the formation of lymphatic vessels and lymphatic metastasis in gastric cancer. It may be an effective way to gastric cancer for the treatments against VEGF-C and its receptors.
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0.05).The transfection efficiencies were improved significantly in the ultrasound plus contrast agent and VEGF plasmid group(MI 1.2,1.4,and 1.6) compared with pure plasmid group(P0.05);there were significant differences between ultrasound plus contrast agent and VEGF165 plasmid(MI 1.4,1.6) and ultrasound plus contrast agent and VEGF165 plasmid group(MI 1.2)(P
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OBJECTIVE:To explore the inhibitory effects and mechanism of Rhizoma Sparganii and Rhizoma Curcumae on the angiogenesis of new granulation tissue in sponge.METHODS:The experimental endometriosis in female rats was established with sponge implantation.The effects of Rhizoma Sparganii and Rhizoma Curcumae on the angiogenesis of new granulation tissue in sponge were observed after intragastric medication for 2 consecutive weeks.The staining of endothelial cell specific Ⅷ factor and vascular endothelial cell growth factor(VEGF) was carried out by immunohistochemistry method.The expression of VEGF mRNA was detected by RT-PCR assay.RESULTS:In rats receiving high dosage of Rhizoma Sparganii and Rhizoma Curcumae(SC),the area of new granulation tissue in sponge,the number of blood vessels,the expression of VE-GF and VEGF mRNA were all significantly lower than in model group(P
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Objective: Astragalus mongholicus could promote angiogenesis.Microemulsion has been widely used in the researches of traditional Chinese medicine in recent years.This study aimed to investigate the effect of Astragalus mongholicus microemulsion modified-collagen on angiogenesis of rats in vivo.Methods: Fifty SD rats were randomly divided into 5 groups: blank collagen,blank microemulsion modified-collagen,vascular endothelial cell growth factor(VEGF) modified-collagen,Astragalus mongholicus microemulsion modified-collagen and Astragalus mongholicus microemulsion plus VEGF modified-collagen.Then four collagen samples were imbedded in each rat.After the establishment of the models,the rats were executed at 3,7 and 14 days.The weights of the collagen samples were measured,and their hematoglobin and hydroxyproline levels were determined by the hemiglobincyanide colorimetric and sampler alkaline hydrolysis methods.The color of the wound surface was observed and the surrounding tissues were examined by HE staining for those killed at 14 days.Results: Both Astragalus mongholicus microemulsion and Astragalus mongholicus microemulsion plus VEGF significantly increased the production of hematoglobin and hydroxyproline(P0.05).Conclusion: Astragalus mongholicus microemulsion might promote angiogenesis by increasing the number of micrangia and the production of hematoglobin and hydroxyproline.