ABSTRACT
Objective To investigate expressions of the vascular endothelial growth factor (VEGF) family and their receptors in cardiac repair/remodeling after myocardial infarction (MI).Methods The infarcted rat heart model were constructed,real time PCR (RT-PCR) and Western blots (WB) were used.Results Compared to the normal myocardium,VEGF-A was significantly decreased in MI group during the 42 days observation period but decreased at day 1,which was 0.89 ±0.04 of control group in D1,0.25 ±0.03 of control in D14; VEGF-B was significantly suppressed in the infarcted heart,which level was 0.09 ± 0.04 of control; However,VEGF-C and VEGF-D were markedly increased in the infarcted heart in MI group,which was 5.31 ± 0.21 and 9.24 ± 0.47 times of control.Meanwhile,VEGFR-1 and 2 were 0.11 ± 0.02 and 0.14 ± 0.04 of control in the infarcted heart,but VEGFR-3 was significantly increased in blood vessels,6.81 ± 0.42 times of control group.Conclusions VEGF isoforms and VEGFR subtypes were differentially expressed in the infarcted heart.It suggests that these isoforms may regulate multiple responses during cardiac repair/remodeling.
ABSTRACT
Objective To analyze tissue vascular endothelial growth factor receptor 1 (VEGFR-1)and VEGFR-2 and their soluble form (sVEFGR-1 and sVEGFR-2) in the plasma in patients with preeclampsia,and to explore its clinical significance.Methods sVEGFR-1 and sVEGFR-2 expressions in plasma of normal pregnancy women and preeclampsia patients were detected by enzyme-linked immunosorbnent assay (ELISA) ; VEGFR-1,VEGFR-2,sVEGFR-1 and sVEGFR-2 mRNA expressions of normal pregnancy women and preeclampsia patients in placenta were detected by reverse transcription-polymerase chain reaction (RT-PCR).Results ELISA results showed that the level of sVEGFR-1 in plasma of normal control group before and after delivery was (12.33 ± 1.52) ng/ml and (4.55 ± 0.31) ng/ml,respectively,while that of preeclampsia group was (77.25 ± 9.47) ng/ml and (8.13 ± 0.74) ng/ml,respectively; the differences between before and after delivery in the two groups were of statistical significance.The level of sVEGFR 2 in plasma of normal control group before and after delivery was (8.74 ± 1.24) ng/ml and (6.43± 0.55) ng/ml,respectively,while that of preeclampsia group was (5.69 ± 0.75) ng/ml and (4.96 ±0.67) ng/ml,respectively.RT-PCR results were consistent with ELISA results.Conclusions Rapid decrease of plasma sVEGFR-1 and continuously low-level expression of plasma sVEGFR-2 indicated that VEGFR-1 might be closely related to preeclampsia,and decrease of plasma sVEGFR-2 in preeclampsia women might be taken as a marker of endothelial cell function disorder.