ABSTRACT
Objective:To discuss the effect of Xiaoqinglong decoction on vascular endothelin (ET) in rats pulmonary hypertension (PAH) model based on the Jiebiaokuoluo method.Methods:Sixty male SD rats were randomly divided into six groups: control group, PAH model group, positive drug group, and Xiaoqinglong decoction in the high-dose, medium-dose and low-dose groups, 10 rats in each group. The control group did not receive any treatment. The remaining groups were kept in low-pressure oxygen tanks, injections of lipopolysaccharide (LPS) at 200 μL on day 1 and day 14, respectively. The rats were smoked twice a day from day 2 to day 30 (except day 14) at 4 hours intervals. The rats were placed in a low-temperature environment for 1 hour per day, and were put into cold water to swim (for 2 weeks), while the rats were given a cold diet. After modeling, the control group and the PAH model group were given equal volumes of normal saline; the positive drug group was given bosentan (100 mg/kg); Xiaoqinglong decoction 15, 10 and 5 g/kg was given, respectively, in the high, medium and low dose groups; once daily for 30 days. The mean pulmonary artery pressure (mPAP) and right ventricle systolic pressure (RVSP) were then measured with right heart catheterization and the right ventricle hypertrophy index (RVHI) was assessed; ET-1 and nitric oxide (NO) levels were determined by radioimmunoassay.Results:Compared with the control group, the levels of mPAP, RVSP, RVHI and ET-1 were significantly increased in the PAH model group [mPAP (mmHg, 1 mmHg≈0.133 kPa): 33.20±1.04 vs. 13.20±1.03, RVSP (mmHg): 62.40±1.54 vs. 24.20±1.02, RVHI: 42.90±2.51 vs. 25.40±2.01, ET-1 (ng/L): 100.80±20.34 vs. 81.50±13.84, all P < 0.05], while NO levels were significantly decreased (mmol/L: 23.20±1.81 vs. 31.70±1.49, P < 0.05). Compared with the PAH model group, the levels of mPAP, RVSP, RVHI and ET-1 in the positive drug group and high, medium, low dose groups of Xiaoqinglong decoction were significantly decreased [mPAP (mmHg): 25.50±0.84, 26.90±0.74, 27.10±1.19, 29.10±0.75 vs. 33.20±1.04, RVSP (mmHg): 54.40±5.14, 50.10±1.67, 53.10±1.05, 56.60±1.07 vs. 62.40±1.54, RVHI: 41.10±1.19, 31.20±1.67, 31.30±1.89, 40.30±1.88 vs. 42.90±2.51, ET-1 (ng/L): 70.70±7.89, 69.90±2.92, 71.70±4.32, 73.90±5.19 vs. 100.80±20.34, all P < 0.05], while NO levels were significantly increased (mmol/L: 32.50±2.06, 34.70±1.16, 32.70±1.33, 30.10±1.19 vs. 23.20±1.81, all P < 0.05), and with the increased dose of Xiaoqinglong decoction, the change was more obvious, and the effect was better in the high-dose group. Conclusion:Xiaoqinglong decoction can reduce rats PAH by regulating ET-1 and NO levels in a dose-dependent manner.
ABSTRACT
Objective To investigate the clinical effect of recombinant human endostatin combined with cisplatin in the treatment of malignant pleural effusion of lung cancer.Methods 100 lung cancer patients with malignant pleural effusion were selected and divided into observation group and control group according to the random number table method,with 50 cases in each group.The control group was treated with cisplatin 40mg/m2 single-dose pleural perfusion.The observation group was treated with 40mg/m2 of cisplatin on the basis of 45mg of recombinant human endostatin.All the patients were treated twice a week,treatment for 3 weeks.The clinical efficacy,adverse reactions,the immune function indicators before and after treatment,quality of life improvement were compared between the two groups.Results (1) In the control group,l0 cases were cured,markedly effective in 24 cases,effective in 12 cases,the total effective rate was 92％.In the observation group,4 cases were cured,markedly effective in 14 cases,effective in 18 cases,the total effective rate was 72％,the difference between the two groups was statistically significant (x2 =9.15,P ＜ 0.05).(2) In the control group,15 cases occurred nausea and vomiting,12 cases of leukopenia,11 cases of neutropenia,10 cases of thrombocytopenia.In the observation group,4 cases of nausea and vomiting,14 cases of leukopenia,10 cases of neutropenia,14 cases of thrombocytopenia.There was no statistically significant difference between the two groups (x2 =10.24,P ＞ 0.05),but the number of adverse reactions in the observation group was less than that in the control group,which indicated that the safety of the observation group was high.(3) Before treatment,the CD4+,CD8+ and CD4+/CD8+ between the two groups had no statistically significant differences (t =0.03,0.26,0.67,all P ＞ 0.05).After treatment,the percentage of CD4+ and CD4+/CD8+ in the observation group were higher than those in the control group,the percentage of CDs+ in the observation group was lower than that in the control group,the differences were statistically significant (t =11.58,12.36,10.84,all P ＜0.05).(4) In the observation group,improved in 31 cases,stable in 10 cases,decreased in 9 cases.In the control group,improved in 25 cases,stable in 12 cases,decreased in 13 cases.There was in statistically significant difference between the two groups (x2 =2.30,P ＜ 0.05).Conclusion Recombinant human endostatin combined with cisplatin in the treatment of pleural effusion of lung cancer is significant,which can enhance the immune function of patients,improve the quality of life of patients,and with good safety,less adverse reactions.
ABSTRACT
Objective To observe the effect of acupuncture therapy of regulating Yin and Yang for the treatment of stroke,and to investigate the influence on plasma levels of calcitonin gene-related peptide(CGRP) and vascular endothelin(ET).Methods One hundred and twenty stroke patients were equally randomized into 3 groups: group A received acupuncture therapy of regulating Yin and Yang,group B received traditional body acupuncture therapy,and group C received scalp acupuncture therapy.One and a half months constituted one treatment course,and the treatment lasted 2 courses.The therapeutic effect was evaluated and the changes of plasma CGRP and ET levels were observed.Results The total effective rate was 95.0% in group A,85.0% in group B,and 82.5% in group C,the difference being significant between the three groups(P0.05).Conclusion Acupuncture therapy of regulating Yin and Yang exerts a certain effect for the treatment of stroke,and can up-regulate plasma CGRP level and decrease ET level.