ABSTRACT
Objective To study the relationship between exon gene polymorphism of pulmonary surfactant pro-tein B(SP -B)and the susceptibility and severity,prognosis of respiratory distress syndrome (RDS).Methods To detect the gene sequence of SP -B exon by adopting the gene sequencing technology,and samples were 80 prematures of very low birth weight in Southern Han Chinese,who were divided into 2 groups,the RDS and the non RDS,and the difference of genotype in SP -B exon in 2 groups was compared.Results There was no significant difference between 2 pretem groups in the aspects of the gestational age,sex,birth weight and delivery mode etc(all P >0.05).Fifty -nine prematures of very low birth weight had exons heterogenesis,and there were 2 types of mutations,V1 :Exon2:c.[5A >C]+[5A >C]or c.[5A >C]+[=];V2:Exon5:c.[428C >T]+[428C >T]or c.[428C >T]+[=].There were 20 cases of type V1 ,1 8 cases of type V2,3 cases of type V1 +V2 in 45 cases of RDS,and there were 1 2 cases of type V1 ,9 cases of type V2,no case of type V1 +V2 in the non RDS group.Comparing the incidence of V1 and V2 in 2 groups,there were all significant differences(χ2 =3.73,5.02;all P 0.05).Conclusion Gene polymorphism of SP -B exon are risk factors for premature of very low birth weight in southern Han Chinese in RDS.
ABSTRACT
BACKGROUND: A delay in spontaneous closure of the patent ductus arteriosus (PDA) is frequent in premature infant and may lead to cardiopulmonary congestion and death. Surgical closure of the PDA in the premature infant can be a safe and effective procedure. Now, several centers prefer to eliminate the problem of transportation to operating room and adopt the policy of operating in the newborn intensive care unit (NBICU). So we investgated the anesthetic management and clinical status of premature infants who underwent surgical closure of PDA. METHODS: We analyzed retrospectively the anesthetic management and clinical status of eleven premature infants below 1,500 g birth weight. RESULTS: Range of gestational age of infants was 24-30 weeks. Most common cause of operation was failure of medical treatment. All infants had features of respiratory distresses and prematurity complications. Fentanyl, vecuronium, oxygen, and air constituted a anesthetic regimen. The body temperature remained stable. Systemic blood pressure with ligation of PDA increased to 66.3 17.4 mmHg (mean+/-SD). After operation, three infants died from complication of prematurity. There were no deaths directly related to operation. Four infants underwent operation in NBICU and also had no wound infections. CONCLUSIONS: Premature infants with PDA had associated complications of prematurity and were severely ill. If the infants did not respond to medical therapy, the PDA was closed by operation with adequate anesthesia. Furthermore, the operation can be performed safely and efficiently in the operating room or NBICU.