ABSTRACT
<b>Preface and Objectives</b><br> Cholera has recently become more serious in African countries. In southern Africa, a serious cholera outbreak was reported in 2008. Here we investigate <i>Vibrio cholerae</i> in aquatic environments, rivers, tube well waters, fish tissues, and aquatic plants in Zambia.<br><b>Methods</b><br> The sampling was conducted on Kafue and Zambezi rivers as well as at public wells. The samples included river water, mud, fish, floating grass, and well water. All samples were enriched in alkaline peptone water (APW) and streaked on thiosulfate citrate bilesalts saccharos (TCBS) Vibrio agar. Some types of colonies were isolated using polymyxin mannose tellurite (PMT) agar. All water samples were analyzed for enterobacteria as well as vibrio contamination. River and shallow tube well water samples were analyzed for <i>E. coli</i>.<br><b>Results</b><br> Samples, including fish tissues, river, and tube well waters, except for the fish intestines, were found to be contaminated with vibrios when cultured on TCBS Vibrio agar.<br> Deep tube well water was not contaminated with vibrios or <i>E. coli. V. cholerae</i> El Tor was not detected through sampling and culturing on PMT agar. <i>E. coli</i> was detected in river and shallow tube well water samples.<br><b>Discussion</b><br> Vibrios were detected in Zambia’s aquatic environments. Cholera outbreak may occur. Vibrio contaminations in fish have been reported from Bangladesh, Laos, Kenya, and India. Fish is a good source of protein to improve person’s nutritional status. However, it must be treated properly so that any contamination of residential areas is avoided. Therefore, people must pay attention to fish.<br><b>Conclusion</b><br> Here several types of <i>V. cholerae</i> were isolated from different aquatic environments. Fish is a nutritious diet, but contaminated Vibrios fish must be treated so that it will not contaminate residential areas. We must investigate the gateway of cholera outbreaks in Zambia.
ABSTRACT
Outbreaks of cholera are common in West Bengal. In April 2006, Garulia municipality reported a cluster of diarrhea cases. We investigated this cluster to identify the etiological agent, source of transmission and propose control measures. We defined a case of diarrhea as occurrence of >3 loose/watery stools a day among the residents of Garulia since April 2006. We searched for cases of diarrhea in health care facilities and health camp. We conducted a gender- and age-matched case–control study to identify risk factors. We inspected the sanitation and water supply system. We collected rectal swabs from diarrhea patients and water specimens from the affected areas for laboratory investigation. Two hundred and ninetyeight cases of diarrhea were reported to various health care facilities (attack rate: 3.5/1000, no deaths). The attack rate was highest among children (6.4/1000). Vibrio cholerae El Tor O1 Inaba was isolated from two of 7 rectal swabs. The outbreak started on 10 April 2006, peaked on 26 April and lasted till 6 May. Cases clustered in an area distal to leaking water pipelines. Drinking municipal water exclusively was significantly associated with the illness (OR 13, 95% CI=6.5–27). Eight of the 12 water specimens from the affected area had fecal contamination and poor chlorine content. This outbreak was due to a contaminated municipal piped water supply and V. cholera 01 Inaba was possibly the causative organism.