ABSTRACT
Objective To investigate the chemical constituents of the whole plants of Viola yedoensis. Methods The chemical constituents were isolated and purified by column chromatography over silica gel and Sephadex LH-20, as well as on the semi-preparative HPLC. The structures of the isolates were identified by the NMR spectroscopic method. Results Twenty-three compounds were isolated and their structures were identified as pubinernoid A (1), (2R,6R,9R)-2,9-dihydroxy-4-megastigmen-3-one (2), 3S,5R-dihydroxy-6R,7-megastigmadien-9-one (3), dehydrovomifoliol (4), blumenol A (5), blumenol B (6), oleanolic acid (7), 2α,3α-dihydroxyurs-12-ene-28-oic acid (8), 1α,2α,3β-trihydroxyolean-12-ene-28-oic acid (9), 2α,19α-dihydroxyursolic acid (10), 3α-hydroxyfriedel-2-one (11), 7-oxopetrosterol (12), 7-oxositosterol (13), syringaresinol (14), lariciresinol (15), daphneticin (16), umbelliferone (17), trans-p-hydroxycinnamic acid methyl ester (18), p-hydroxyphenylpropionic acid (19), p-hydrobenzaldehyde (20), p-methoxybenzaldehyde (21), p-methoxybenzoic acid (22), and 5-hydroxymethyl-2-furfural (23). Conclusion Compounds 1-16 and 18-23 are isolated from the genus Viol for the first time, and compound 17 is isolated from the plant for the first time.
ABSTRACT
Objective:To establish an HPLC method for the simultaneous determination of chlorogenic acid , aesculetin, rutin, acacetin-7-O-β-D-glucoside, quercetin and luteolin in the extract of Viola yedoensis Makino.Methods:The HPLC analysis was carried out on a Hypersil ODS C18 column (250 mm ×4.6 mm, 5μm) with 0.1％phosphoric acid (A)-methanol (B) as the mobile phase with gradient elution at the flow rate of 1.0 ml· min-1 .The detection wavelength was 345 nm and the column temperature was 35℃. Results:Good linear relationship was found within the range of 4.3175-172.7000 mg· L-1 for chlorogenic acid, 2.7350-109.4000 mg· L1 for aesculetin, 6.9800-279.2000 mg · L-1 for rutin, 3.7200-148.8000 mg · L-1 for acacetin-7-O-β-D-glucoside, 4.1350-165.4000 mg· L-1 for quercetin, and 3.3950-135.8000 mg · L-1 for luteolin.The average recovery was 99.06％, 98.84％, 98.77％, 99.40％, 98.53％and 98.71％, respectively.The content of chlorogenic acid , aesculetin, rutin, acacetin-7-O-β-D-glucoside, quercetin and luteolin in the extract of Viola yedoensis Makino was 1.6290, 1.1910, 4.5850, 2.2810, 3.1790 and 1.9710 mg· g-1, respectively.Conclusion:The method is accurate and reliable with good reproducibility , which can be used for the quality control of the extract of Viola yedoensis Makino.
ABSTRACT
The different extracts and of Viola yedoensis Makino and Viola inconspicua were analyzed and identi-fied by Fourier transform infrared spectroscopy (FT-IR). One-dimensional infrared spectrum showed that the extracts of Viola yedoensis Makino and Viola inconspicua contained the aromatics,volatile substances and glyco-sides,with not significant differences from each other. However,different extraction sites of the two medicinal materials in second derivation spectrum were obviously different,especially the number of automatic peaks and peak intensity in the range of 970 800 cm-1. Viola yedoensis Makino displayed 5 automatic peaks,6 automatic peaks and 6 automatic peaks,while Viola inconspicua displayed 7 automatic peaks,4 peaks,4 peaks in the second derivation spectrum of petroleum ether extraction site,chloroform extraction site and the ethyl acetate extraction site. In addition,the peak position of the strongest peak in the second derivative of the ethyl acetate extraction site was 1 467 cm-1,while the strongest peak of the Viola inconspicua was at 1 384 cm-1,so the two medicinal mate-rials can be distinguished by the strongest peak position of ethyl acetate extraction site in second derivation spec-trum. Studies demonstrated that one-dimensional infrared spectroscopy combined with the second-order derivative analysis could achieve the accurate identification between Viola yedoensis Makino and Viola inconspicua. This research provides new ideas and new methods for the identification of Viola and other adulterants.
ABSTRACT
AIM:To analyse the chemical constituents of volatile components from Viola yedoensis Makino. METHODS: The essential oils from Viola yedoensis Makino(collected from Jinhua city and Changchun city) were extracted by steam distillation.The chemical compounds were separated and identified by GC-MS.The relative content of each component was determined by area normalization. RESULTS: Twenty-five and twenty-three constituents were separated from Viola yedoensis Makino collected from Changchun city and Jinhua city;twenty-one and twenty-two compounds were identified respectively.The ratios of identification of total compounds were 88.00% and 91.30% respectively. CONCLUSION: There are significant difference between two varieties of Viola yedoensis Makino,the main coustituents in each plant are n-hexadecanoic acid(16.77%)and phytol(26.97%).