ABSTRACT
ObjectiveTo observe the dynamic changes of peripheral blood T lymphocytes and monocytes,which serve as HIV-1 viral reservoirs,in Chinese HIV-infected patients receiving highly-active antiretroviral treatment ( HAART ) for 48 weeks and its clinical significance. MethodsA total of 35 chronic HIV-1 infected adults naively received HAART.The peripheral blood T lymphocyte subsets counts were determined by flux cytometry at week 0,24 and 48.Magnetic activated cell sorting was used to extract cellular DNA from monocytes and T lymphocytes purified from peripheral blood mononuclear cells.Real-time fluorescent quantitative PCR was used to detect the serum HIV RNA and HIV DNA of monocytes and T lymphocytes.SPSS 18.0 software was used to analyze the collected data.ResultsAt week 0,24,and 48 after initiation of HAART,HIV RNA levels of peripheral blood were (4.12 ± 1.41 ),≤1.69,and ≤1.69 lg copies/ml,respectively ; CD4+ T cells were ( 196 ± 101 ),(321.90 ± 112) and ( 392 ± 127 ) cells/μl,respectively ; HIV DNA level in T lymphocytes were ( 4.03 ± 0.53 ),( 2.74 ± 1.16 ) and ( 2.45 ± 0.41 )lg copies/106 cells respectively; while in monocytes,HIV DNA levels were (2.51 ±0.68),(2.16 ±0.34) and (2.03 ± 0.25 )lg copies/106 cells.Statistical analysis revealed that HIV RNA level was negatively correlated with the CD4+ T cell count through the whole trail,while positively correlated with the HIV DNA level in blood T lymphocytes and monocytes.HIV DNA level in T lymphocytes decreased more slowly than HIV DNA in monocytes. Moreover,peripheral blood CD4+ T cell count was negatively associated with the HIV DNA capacity from T lymphocytes.ConclusionsBoth T lymphocyte and monocyte may serve as viral reservoirs,and T lymphocyte might play a more important role as HIV reservoirs. The blood HIV RNA is correlated positively with the cellular HIV DNA,whereas,CD4+ T cell count is correlated negatively with HIV DNA from lymphocytes,which suggests that HIV DNA levels in T lymphocyte might be one of indicators of AIDS progress during HAART.
ABSTRACT
O vírus da imunodeficiência humana causa infecção persistente apesar da terapia com antirretrovirais. Isso leva a crer que existem outros reservatórios virais além dos já conhecidos. Estudos recentes mostram que o vírus pode invadir as células progenitoras hematopoiéticas, causar infecção ativa assim como latente e morte celular. Essas célulasinfectadas latentemente podem persistir por vários anos e contribuir para a viremia residual e a persistência viral. A morte celular causada pela invasão e replicação do vírus pode estar associada a anormalidades hematopoiéticas. Palavras-chave. Células progenitoras hematopoiéticas; vírus da imunodeficiência adquirida; reservatórios virais
Human immunodeficiency virus (HIV) causes persistent infection despite antiretroviral therapy. This suggests the existence of other viral reservoirs beyond those already known. Recent studies show that HIV can invade hematopoietic progenitor cells, generating active infection as well as latent infection and cell death. The latently infected HPCs may persist for several years and contribute to residual viremia and viral persistence. Cell death caused by the invasion and replication of the virus may be associated with hematopoietic abnormalities.