ABSTRACT
Ebola virus (EBOV) is a highly lethal virus leading to rapidly fatal hemorrhagic fever in humans and other vertebrates, whose mortality rate could be 90%. Up to now, there is still no effective anti-virus drug or vaccine. Because of severe public health hazard, EBOV has been listed in the most dangerous viruses to humans by WHO. Due to the outbreak of Ebola hemorrhagic fever (EHF) in Africa in 2014, the correlation research on EBOV has become the hot topic in virology. The general characteristics, structure composition, life cycle of virus, pathogenesis generalization, clinical manifestation of EHF were described in this review. The recent advances about the antiviral targets and drugs were also summarized, as well as prospect on the application of Chinese materia medica in anti-EBOV. In a word, the understanding of EBOV and drug development could be promoted by this review.
ABSTRACT
Several examples will be described in which powerful separation methods are combined with relatively simple chemical modification techniques to provide structural information on complex macromolecular assemblies. Ribosomal RNA structure has been examined by crosslinking, separating individual crosslinked species by gel electrophoresis, and enzymatic methods for determination of crosslink positions in the nucleotide sequence. Chromatin structure has been examined by footprinting the location of individual nucleosomes by a combination of chemical nicking and DNA separations. Virus structure can be examined by using breakable crosslinkers analyzed with diagonal gel electrophoresis. Ultimately such methods may allow structural information to be obtained on systems even as complex as whole chromosomes.