ABSTRACT
Objective To provide experimental basis for the identification of Vitex negundo var. cannabifolia fruits and V. negundo fruits by comparative study. Methods The original plant identification, morphological identification, microscopic identification, TLC and HPLC identification were adopted to distinguish the two kinds of materials. Results It is difficult to tell the difference of these two medicinal fruits in term of original plant identification and morphological identification to their similarity, while microscopic identification, TLC and HPLC identification revealed that the characteristics of sclereid could be used to distinguish these two fruits. Conclusion Microscopic identification on the sclereid characteristics of fruits powder, combined with TLC and HPLC identification can be the feasible method that used to distinguish V. negundo fruits and V. negundo var. cannabifolia fruits.
ABSTRACT
Objective: To study the chemical constituents in the twigs of Vitex negundo var. heterophylla. Methods: A variety of silica gel column chromatography, Sephadex LH-20 gel column chromatography, and HPLC methods were used for the separation and purification of chemical composition. Their structures were identified on the basis of physicochemical property and spectral data. Results: Eight compounds were obtained and identified as apigenin-6-C-β-D-glucopyranosyl 8-C-α-L-arabinopyranoside (1), isoorientin (2), luteolin-6-C-α-L-arabinopyranosyl-8-C-β-D-glucopyranoside (3), luteolin-6,8-di-C-α-L-arabinopyranoside (4), apigenin-6-α-L- arabinopyranosyl-8-C-β-D-glucopyranoside (5), luteolin-6-C-α-L-arabinopyranosyl 8-C-β-L-arabinopyranoside (6), luteolin-6-C-β-L- arabinopyranosyl-8-C-β-D-glucopyranoside (7), and luteolin-7-O-β-D-glucopyranoside (8). Conclusion: Compounds 1 and 3-7 are first isolated from the plants of Vitex Linn.
ABSTRACT
Objective: To investigate the flavonoid glycosides from the leaves of Vitex negundo var. cannabifolia. Methods: Column chromatography including silica gel, Sephadex LH-20, and ODS was used to separate and purify the chemical constituents, and their structures were elucidated by physicochemical properties, MS, and NMR spectroscopic data. Results: Seven flavonoid glycosides were obtained from the ethyl acetate layer of 95% EtOH extract of the leaves of V. negundo var. cannabifolia, and identified as luteolin-4'-O-(6″-O-p-hydroxybenzoyl)-β-D-glucoside (1), luteolin-7-O-(6″-O-p-hydroxybenzoyl)-β-D-glucoside (2), luteolin-6-C-(6″-O-trans-caffeoyl)-β-D-glucoside (3), luteolin-6-C-(2″-O-trans-caffeoyl)-β-D-glucoside (4), perfoliatumin A (5), isovitexin (6), and luteolin-7-O-β-D-glucoside (7). Conclusion: Compound 1 is a new compound named cannabifolin G; Compounds 2-4 and 7 are obtained from this plant for the first time; Compound 5 is firstly isolated from the plants in Vitex L.
ABSTRACT
Objective: To investigate the chemical constituents from Vitex negundo. Methods: The chemical constituents were separated and purified by column chromatography including ODS, Sephadex LH-20, and silica gel columns. Their structures were elucidated on the basis of physicochemical properties and spectral data. Results: Eleven compounds were identified as isoorientin (1), vitegnoside (2), luteolin-7-O-β-D-glucopyranoside (3), isovitexin (4), luetolin-3'-O-β-D-glucuronide (5), apigenin-7-O-β-D-glucoside (6), kaempferol-3-O-β-D-glucopyranoside (7), methyl rosmarinate (8), 5-O-caffeoyl quinic acid methylester (9), caffeic acid (10), and grevilloside G (11), respectively. Conclusion: Compounds 3 and 4 are isolated from V. negundo for the first time, and compounds 5-11 are firstly obtained from the plants of Vitex Linn.