ABSTRACT
Objective:To analyze the changes of chemical compositions and relative contents of volatile constituents in raw Xanthi-um sibiricum Patr. and fried Xanthium sibiricum Patr. Methods: Headspace solid phase micro extraction ( HS-SPME) combined with gas chromatography-mass spectrometry ( GC-MS) was used to study the volatile components in raw and fried products of Xanthium sibir-icum Patr. . A peak area normalization method was applied to calculate the relative percentage content of each composition. Results:Totally 26 and 27 kinds of compounds were isolated and identified respectively from raw and fried xanthium including 19 species and re-spectively accounting for 68. 69% and 90. 08% of the total volatile components. In the raw products, the components with high con-tents were camphor (15. 3%), eucalyptus oil alcohol (9. 6%) and borneol (6. 89%), while in the fried products, those were men-thol (24. 09%), borneol (18. 48%) and eucalyptol (7. 19%). Conclusion:The relative contents of components with effects on rhi-nitis in the fried products are obviously higher than those in the raw products. HS-SPME-GC-MS can be used to detect the volatile com-positions and their relative contents in the raw products and fried products of Xanthium sibiricum Patr. . From the changes of volatile components, theoretical basis can be laid for the components with certain efficacy in raw and fried Xanthium sibiricum Patr. , and scien-tific basis can be provided for their further study and comprehensive utilization.
ABSTRACT
ThevolatilecompositionsofDuchesneaindicawerestudiedbyheadspacesolid-phase microextraction ( HS-SPME ) , soxhlet extraction ( SE ) , ultrasonic assistant extraction ( UAE ) and steam distillation ( SD) coupled with gas chromatography-mass spectrometry ( GC-MS) . The experimental parameters of HS-SPME, including fiber type, extraction temperature, extraction time and desorption time were investigated. 47, 32, 16 and 16 compounds were identified by HS-SPME, SD, SE and UAE extracting methods, respectively. 66 compounds were obtained in total, among which 47 compounds were first reported in Duchesnea indica. The experimental results showed that terpenoids were the most abundant compositions in HS-SPME and SD, but acids accounted for 61. 44% and 69. 54% of the total content obtained by SE and UAE.