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【Objective】 To understand the antigen profile and antibody frequency of Wra in voluntary blood donors in Shaanxi province. 【Methods】 Wra antigen and antibody screening as well as blood group typing and antibody identification were performed by serological tests and confirmed by genetic testing. 【Results】 The incidence of Wra antigen in 7 490 voluntary blood donors was 0.013%(1/7 490), and the frequency of anti-Wra in 729 voluntary blood donors was 0.823%(6/729). 【Conclusion】 This study explored the polymorphism of Wra antigen and antibodies in blood donors, which is informative in the risk assessment of blood transfusion and the screening and identification of respective antibodies.
ABSTRACT
Background: Emergency Contraception (EC) is an important option for women exposed to unprotected sex to prevent unintended pregnancy and induced abortion. EC has a potential to offer women an important option for fertility control. The objective of the study was to assess knowledge about Emergency Contraception among currently married women of reproductive age in a rural area of West Bengal. Methods: A community based cross sectional study was conducted on 608 currently married women of reproductive age for 1 year from May 2017 to April 2018. Questionnaire was administered to women on knowledge of emergency contraception (EC). Data was analyzed in SPSS version 16. Results: Among the study participants 66% had knowledge of emergency contraception. A small number (8.9%) of the total participants ever used EC to prevent pregnancy. Only 65.3% of them had correct knowledge regarding timing of EC. Effectiveness of post-coital pill was known to them while none of these women were aware of the existence of Intra Uterine Contraceptive Device (IUCD) insertion as an option for EC. Conclusions: Marked lacunae were observed regarding knowledge and practice of emergency contraceptives among the WRA. Proper and correct knowledge on Emergency Contraception is of urgent need among all sexually active women especially in this country for population stabilization.
ABSTRACT
BACKGROUND: Wra is a low-incidence antigen, which is antithetical to the high prevalence red blood cell antigen, Wrb. Anti-Wra is a naturally occurring antibody that is found in approximately 1-2% of blood donors. The aim of this study was to determine the frequency of Wra and anti-Wra in Brazilian blood donors.METHODS: A total of 1662 Brazilian blood donors were molecularly analyzed using the SNaPshot methodology to determine the WR*A/B alleles and to predict the frequency of the Wra antigen. To detect the anti-Wra, samples from 1049 blood donors were analyzed using a gel test with Wr(a+) red blood cells. The serum was treated with dithiothreitol (DTT) to determine the immunoglobulin classes. Immunoglobulin (Ig)-G isotype classification was performed in a gel test using the IgG1/IgG3 card. A monocyte monolayer assay was employed to predict the clinical significance of IgG anti-Wra.RESULTS: Of the 1662 donors, only one sample had the DI*02.03 allele in heterozygous predicting the Wr(a+b+) phenotype. Anti-Wra was detected in 34 (3.24%) samples, 64.7% in females and 35.3% in males. Regarding the immunoglobulin class, eight (23.5%) cases of anti-Wra were classified as IgG and 26 (76.5%) as IgM. Of the eight cases of IgG anti-Wra, four were IgG1, two were IgG3 and three anti-Wra were not IgG3 or IgG1, and thus probably IgG2 or IgG4. The results of the monocyte monolayer assay showed that IgG anti-Wra might be of clinical significance.CONCLUSION: This study shows a very low frequency (0.06%) of the Wra antigen in Brazilian blood donors. Additionally, it shows that the frequency of anti-Wra in this population is higher than previously reported.
Subject(s)
Humans , Blood Donors , Blood Group Antigens , Gene FrequencyABSTRACT
Authors found anti-Wra antibody in a 44-year-old female patient with uterine myoma for the first time in Korea. Since anti-Wright antibodies have been implicated in hemolytic transfusion reactions and hemolytic disease of the newborn, Wra positive cells should be included in the antibody screening panel in Korea.