ABSTRACT
Esta investigación tuvo como objetivo principal evaluar la eficiencia de la estación para tratamiento de aguas residuales de la ciudad Santa Fé do Sul (São Paulo) y definir posibles efectos en la salud pública producidos por la calidad del efluente final. Se realizó un estudio batimétrico de las lagunas de estabilización anaerobia con bafle divisor y facultativa para formar un perfil de acumulación de lodos; se monitoreó durante 24 horas consecutivas el afluente bruto y los efluentes de las lagunas para diagnosticar el comportamiento de la Estación. La eficiencia media de remoción de la demanda bioquímica de oxígeno fue del 89%, mayor que la mínima eficiencia exigida por la legislación brasilera vigente. La cantidad de coliformes fecales superó en gran medida los valores permitidos; sin embargo, la concentración de sólidos sedimentables cumplió a cabalidad con la norma ambiental presentando valores menores a 1,0 mL/L. Los resultados mostraron que la estación requiere labores de mantenimiento de la laguna anaerobia, así como mayor control operacional y mantenimiento más efectivos. Se evidenció la necesidad de implementar un sistema de pos tratamiento que garantice la reducción de los coliformes fecales del efluente final como medida para disminuir posibles riesgos a la salud pública en la zona de descarga y aguas abajo del punto de vertimiento final.
The main objective in this research was to evaluate the efficiency of the sewage treatment plant of Santa Fé do Sul city (São Paulo) and to define possible effects on public health produced by the final effluent quality. A bathymetric study of the anaerobic with baffle and facultative stabilization ponds was done in order to define the sludge accumulation profile; the raw influents and the lagoons effluents were monitored during 24 consecutive hours to diagnose the Plant performance. The average efficiency of the biochemical oxygen demand was 89%, higher than the minimum efficiency allowed by the Brazilian current legislation. The amount of fecal coliforms highly exceeds the permitted values; however the settleable solids concentration achieved the environmental regulation with values lower than 1, 0 mL/L. The results indicated that the station requires maintenance works at the anaerobic pond, a better operational control and more effective maintenance. It was shown the need to install a post treatment system to guarantee the reduction of the fecal coliforms at the final effluent as an action to diminish the public health possible risks in the discharge zone and downstream of the final dump point.
ABSTRACT
The numbers of Pseudomonas sp. isolated were counted in samples collected from water, sludge and intestine of fishes raised in different wastewater ponds along an effluent gradient in a sewage treatment plant. Total fish yield in the last maturation pond increased by 73% over the yield in first maturation pond or facultative pond. The number of Pseudomonas sp. isolated from the intestine of the tilapia (Oreochromis mssambicus) raised in facultative pond, was more than three times the counts (7.22 × 108/g) observed in the last maturation pond (2.025 × 108/g). The effective lethal concentration of cadmium for Pseudomonas sp. isolated from the intestine of the tilapia was 0.6 mM and 0.08-0.09 mM when the fish was procured from facultative pond and last maturation pond, respectively. The Pseudomonas sp. isolated from the intestine of the tilapia did not have resistance to any of the ten antibiotics tested. However, the bacterium isolated from raw sewage, water and sediment of the anaerobic pond was resistant to seven out of ten antibiotics tested.
ABSTRACT
Esta pesquisa teve como objetivo principal comparar cinco métodos espectrofotométricos para determinação de proteínas em amostras provenientes de estações de tratamento de efluentes sanitários. O intuito foi definir uma metodologia de aplicação rápida, fácil e confiável para este tipo de amostra. As lagoas de estabilização, como sistemas de tratamento biológico, têm como principais constituintes proteínas, carboidratos e lipídeos, mas também apresentam muitos compostos interferentes, como por exemplo, uréia, detergentes e compostos fenólicos, que podem prejudicar a quantificação de tais parâmetros. Os métodos analisados foram Lowry, Biureto, Bradford e Ácido bicinconínico. O método de Lowry mostrou-se mais adequado às características da amostra, com boa reprodutibilidade, reagente específico, custo moderado e ausência de substancias interferentes.
This research had as main objective to compare five spectrophotometric methods for protein determination in samples proceeding from sanitary effluent of treatment plant. Intention was to define a methodology that is of fast and easy and reliable application for this type of sample. The stabilization ponds, as systems of biological treatment, have as main constituent proteins, carbohydrates and lipids, but also they present many interfering composites, for example, phenolic urea, detergents and composites, that can harm the quantification of such parameters. The analyzed methods had been Lowry, Biuret, Bradford and Acid bicinconinic. The method of Lowry revealed more adequate to the characteristics of the sample, with good reproducibility, specific reagent, moderate cost and absence of interfering substance.
ABSTRACT
Esta pesquisa teve como principal objetivo investigar os pro-cessos envolvidos nas transformações do nitrogênio em um sistema de lagoas de estabilização. Em amostragem nictemeral (24 horas) observou-se forte estratificação térmica durante a maior parte do ciclo amostrado a qual condicionou a compartimentalização vertical e a estratificação química nas duas lagoas, possibilitando a análise dos processos de forma segmentada pela ACP (análise de componentes principais), que mostrou-se ferramenta estatística muito útil na caracterização dos processos. A degradação de proteína, com subseqüente geração e acúmulo de nitrogênio amoniacal, foi observada, além da possível excreção de proteína ou aminoácidos por organismos fitoplanctônicos na superfície das lagoas. A provável ocorrência de nitrificação na superfície da lagoa anaeróbia evidenciou a necessidade de otimização de operação do sistema a fim de viabilizar a remoção de nitrogênio através da desnitrificação, processo provavelmente inibido pelas elevadas concentrações de oxigênio dissolvido registradas durante o dia.
This research had as main objective to investigate the involved processes in the transformations of nitrogen in a system of waste stabilization ponds. In nictemeral sampling (24 hours) strong thermal stratification was observed during most of the showed cycle which conditioned two vertical compartments and the chemical stratification in the two lagoons, making possible the analysis of the processes of segmented form for PCA (Principal Components Analysis) that was a very useful statistics tool in the characterization of the processes. The protein degradation was observed with subsequent generation and accumulation of ammoniacal nitrogen, beyond the possible excretion of protein or amino acids for phytoplanktonic organisms in the surface of the ponds. The probable occurrence of nitrification in the surface layer of the anaerobic pond evidenced the necessity of improving the operation parameters of the system in order to reach a greater nitrogen removal through the denitrification, process probably inhibited by significant high concentrations of oxygen dissolved registered in superficial layers, particularly during the day.