ABSTRACT
OBJECTIVE: To establish a method for simultaneous determination of adenine, cytidine, inosine, uracil, uridine, thymidine, adenosine and 2-deoxyadenosine in Zhejiang Fritillary Slices, and determine and compare the contents of the eight compositions in Zhejiang Fritillary Slices before and after sulfur fumigation process. METHODS: The samples solution was determined by HPLC-DAD with an Inertsil ODS-SP C18 column (4.6 mm×250 mm, 5.0 μm) using water (A)-methanol (B) as the mobile phase by gradient elution (0-10 min, 1%→5% B; 10-15 min, 5%→15% B; 15-20 min, 15%→20% B; 20-30 min, 20% B; 30-35 min, 20%→100% B) at the flow rate of 1 mL·min-1. The column temperature was maintained at 25℃. RESULTS: All the components showed good linearity (r≥0.9995) in the range of the tested concentration. The average recoveries of the method were 100.90%, 97.92%, 100.28%, 98.95%, 100.42%, 99.02%, 101.96% and 100.39%, respectively. After sulfur-fumigation process, the contents of the six compositions in Zhejiang Fritillary Slices decreased obviously. CONCLUSION: The validated method has the advantages of simpleness, high precision and reliability, allowing the comprehensive quality control of Zhejiang Fritillary Slices. Sulfur fumigation can remarkably affect the amount of the eight water-soluble constituents in Zhejiang Fritillary Slices. So, sulfur fumigation shouldn't be apllied as primary processing method for Zhejiang Fritillary Slices.
ABSTRACT
OBJECTIVE: To study water-soluble constituents from the seeds Orychophragmus violaceus. METHODS: The constituents were isolated and purified by silica gel chromatography repeatedly, and the structures were identified by spectral analysis and chemical methods. RESULTS: Fifteen water-soluble compounds were isolated from the seeds Orychophragmus violaceus and their structures were identified as blumenol A(1), 3', 5', 5, 7-tetrahydroxylflavanone(2), (+)-catechin(3), quercetin(4), adenosine(5), salicylic acid(6), 2'-deoxy adenosine(7), p-coumaric acid(8), vanillic acid(9), protocatechuic acid(10), caffeic acid(11), syringic acid(12), daucosterol(13), succinic acid(14) and azelaic acid(15). CONCLUSION: All compounds are isolated from Orychophragmus violaceus for the first time. This study can establish the foundation for the classification chemical composition this plant.