ABSTRACT
Objective To give specific strategies for discipline construction in the "first class"universities and colleges, through the analysis of the SCI service of 20 independent medical universities in China during 2013 to 2017. Methods Based on WoS and ESI database, this paper searches and analyzes the related data of SCI papers published by the above colleges and universities, including the quantity of output, the citation of all articles, the fund support and other bibliometrics indexes, and explores the status and problems of these SCI papers. Results All colleges and universities in this study have entered the ESI database, and the ranking of ESI is basically the same as the global ranking of US News and ARWU;the Clinical Medicine and Pharmacology&Toxicology of some colleges and universities has been in or near the global 1 per thousand of ESI, and the proportion of the quoted volume and the total volume of the document is still lower than the average level in China;the H index is directly proportional to the papers, and there is still a gap with the first-class universities in China. Most papers are funded by national fund projects, but a large number of SCI articles are not highly influential. Conclusion The results show that the global ranking of independent medical universities in China has developed rapidly in the past five years. Although there is still a gap with the international first-class universities and disciplines, but there is great potential . The level of Clinical Medicine and Pharmacology & Toxicology has reached the international first-class level. The gap between the development level of biology and biochemistry, neuroscience and behavior, and the international medical level of these field is narrowing.
ABSTRACT
To screen molecular chaperones similar to small heat shock proteins (sHsps), but without alpha-crystalline domain, heat-stable proteins from Schizosaccharomyces pombe were analyzed by 2-dimensional electrophoresis and matrix assisted laser desorption/ionization time-of-flight mass spectrometry. Sixteen proteins were identified, and four recombinant proteins, including cofilin, NTF2, pyridoxin biosynthesis protein (Snz1) and Wos2 that has an alpha-crystalline domain, were purified. Among these proteins, only Snz1 showed the anti-aggregation activity against thermal denaturation of citrate synthase. However, pre-heating of NTF2 and Wos2 at 70degrees C for 30 min, efficiently prevented thermal aggregation of citrate synthase. These results indicate that Snz1 and NTF2 possess molecular chaperone activity similar to sHsps, even though there is no alpha-crystalline domain in their sequences.