ABSTRACT
Objective:To establish a method for the determination of chloramphenicol residue in Wuji Baifeng pills by liquid chro -matography tandem mass spectrometry with normal solid-phase extraction .Methods:A normal solid-phase extraction column was used for the sample pretreatment and enrichment , and high performance liquid chromatography tandem mass spectrometry was used to deter-mine chloramphenicol residue in Wuji Baifeng pills .The analysis was performed on an Agilent-ODS C18 (250 mm ×4.6 mm,5 μm) column .The mobile phase was composed of methanol and water with gradient elution at 40℃ (0-7 min, 30% methanol;7-15 min, 30%-80%methanol;15-25 min, 80%-30%methanol).The flow rate was 1.0 ml· min-1 , and the quantitation ion was m/z 152.1 (the negative ionization) under the mode of multiple reaction monitoring .Results:The limit of detection was 0.016 ng.The calibra-tion curves were linear within the range of 0.187-3.749 ng.The method recovery was 94.3% with RSD of 3.19%(n=9).Conclu-sion:The method is simple with accurate results .It is suitable for the determination of chloramphenicol residue .
ABSTRACT
OBJECTIVE:To observe the clinical efficacy of Hepatocyte growth-promoting factors(PHGF) combined with Wuji baifeng pills for liver cirrhosis. METHODS:A total of 97 patients with liver cirrhosis were randomly assigned to either control group(n=32,conventional therapy) or treatment group(n=65,PHGF plus Wuji baifeng pills in addition to routine treatment).The course of treatment was 3 months for both groups. Clinical data including cardinal symptoms and signs,hepatic function,blood clotting function,hepatic fibrosis parameters,the inner-diameter of the portal vein and splenic vein(PVD,SVD),spleen thickness(SPT) measured by the color Doppler ultrasonography were monitored before and after treatment. RESULTS:After treatment,the symptoms and signs of the treatment group had better improvement than in the control group,with a markedly higher total effective rate than in the control group(84.62% vs.59.38%,P
ABSTRACT
AIM: To develop a method for determining the contents of peoniflorin,ferulaic acid and paeonol in Shi'er Wuji Baifeng Pills(Gallus Domesticus,Radix Astragali,Rhizoma Dioscoreae,Radix Codonopsis,etc.) METHODS: The analytical column was an ODS column.The mobile phase consisted of acetonitrile-0.1% phosphoric acid aqueous solution using a gradient elution.The detection wavelengths of peoniflorin,ferulaic acid and paeonol were at 230,320 and 274 nm,respectively. RESULTS: The calibration curves of peoniflorin,ferulaic acid and paeonol showed good linearity at the ranges of 21.19-211.90,1.01-10.06,10.63-106.30 ?g/mL and the average recoveries were 98.63%,98.35%,99.12%,respectively. CONCLUSION: The method is simple,rapid,reliable and can be used for the drug control.