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1.
Chinese Traditional and Herbal Drugs ; (24): 1379-1384, 2018.
Article in Chinese | WPRIM | ID: wpr-852114

ABSTRACT

Objective: To study the protective effect of Wuzi Yanzong Prescription (WYP) on DNA damage in testis of natural ageing rats based on P53/P21 signaling pathway and base excision repair (BER). Methods: SPF grade 16-month-old male SD rats were randomly divided into three groups with eight rats in each group: ageing model group, low and high dose of WYP groups (1 and 4 g/kg). In addition, 2-month-old SD male rats were used as adult control group. The ageing model group and the adult control group were fed with normal diet for four months. Rats in the WYP groups were given the medicated feed for four months. After fasting for 12 h, the rats were sacrificed. Then, the testes were immediately removed from rats. The expression and localization of DNA damage-related protein γH2AX and BER-related proteins OGG1, APE1, and XRCC1 were detected by immunofluorescence, and the content of 8-OHdG in testis was detected by ELISA. Also, the protein expression levels of p-P53 and P21 were detected by Western blotting. Results: Compared with the ageing model group, immunofluorescence results showed WYP significantly decreased the expression levels of DNA damage-related protein γH2AX and BER-related proteins OGG1, APE1, and XRCC1 in testis of natural ageing rats. ELISA results showed that WYP significantly downregulated 8-OHdG levels, compared with the ageing model group. Moreover, Western blotting results showed that WYP significantly decreased the protein expression levels of p-P53 and P21 of the testis when compared with the ageing model group. Conclusion: WYP reduced the DNA damage of testes in ageing related rats via P53/P21 and BER pathways.

2.
Chinese journal of integrative medicine ; (12): 117-124, 2018.
Article in English | WPRIM | ID: wpr-327223

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the relationship between tissue distributions of modified Wuzi Yanzong prescription (, MWP) in rats and meridian tropism theory.</p><p><b>METHODS</b>A high-performance liquid chromatography with Fourier transform-mass spectrometry (HPLC-FT) method was used to identify the metabolites of MWP in different tissues of rats after continued oral administration of MWP for 7 days. The relationship between MWP and meridian tropism theory was studied according to the tissue distributions of the metabolites of MWP in rats and the relevant literature.</p><p><b>RESULTS</b>Nineteen metabolites, mainly flavanoid compounds, were detected in the different rat tissues and classified to each herb in MWP. Further, it was able to establish that the tissue distributions of the metabolites of MWP were consistent with the descriptions of meridian tropism of MWP available in literature, this result might be useful in clarifying the mechanism of MWP on meridian tropism. In the long run, these data might provide scientific evidence of the meridian tropism theory to further promote the reasonable, effective utilization, and modernization of Chinese medicine.</p><p><b>CONCLUSION</b>The tissue distributions of MWP in vivo were consistent with the descriptions of meridian tropism of MWP.</p>

3.
Chinese Traditional Patent Medicine ; (12): 466-470, 2017.
Article in Chinese | WPRIM | ID: wpr-515121

ABSTRACT

AIM To investigate the effects of Wuzi Yanzong Prescription (Lycii Fructus,Cuscutae Semen,Rubi Fructus,Plantaginis Semen and Schisandrae chinensis Fructus) on DNA damage of testis cells in adult male mice induced by cyclophosphamide (CTX).METHODS Forty out of fifty adult male Balb/C mice were injected intraperitoneally with CTX and then were randomly and equally divided into model control group (normal saline),Wuzi Yanzong Prescription low-,medium-and high-dose groups (100,200 and 400 mg/kg),and the other ten mice served as normal control group (normal saline).All mice were anesthetized by inhalation of ether,and then were sacrificed by cervical dislocation.The sperm count,sperm motility and malformation rate of sperm were tested.The content of 8-hydroxy-deoxyguanosine (8-OHdG) in serum was measured using ELISA;the DNA damage degree of cells in testis was detected by single cell gel electrophoresis;the protein expressions of p-P53 and γ-H2AX in testis were examined by Western blot.RESULTS Compared with the model control group,Wuzi Yanzong Prescription groups showed significant increase in the sperm count,sperm motility and significantly decreased malformation rate of sperm,the level of 8-OHdG in serum,and the protein expressions of p-P53 and γ-H2AX in testis were also significantly decreased.CONCLUSION Wuzi Yanzong Prescription can significantly alleviate the DNA damage of testis cells in mice induced by CTX through down-regulating protein expressions of p-P53 and γ-H2AX.

4.
Chinese Pharmaceutical Journal ; (24): 1874-1879, 2015.
Article in Chinese | WPRIM | ID: wpr-859313

ABSTRACT

OBJECTIVE: To study the effect of modified wuzi-yanzong prescription(MWP) on brain gene expression profile in senescence accelerated mouse-prone/8 (SAMP8) mice and detect the mechanism of MWP treating dementia-related diseases. METHODS: Six SAMP8 mice were randomly divided into model group and MWP group on average, meanwhile three senescence accelerated mouse-resistance/1 (SAMR1) mice were chosen as the blank control group. The MWP group was intragastrically intervened by MWP 9 g · kg-1 · d-1, while model group and control goup were given equal volume of sodium carboxyl methyl cellulose, once a day. After 10 d, the mice in experiment were killed and seperated the whole brain. Brain RNA expression was analyzed using Illumina whole genome expression profiles. RESULTS: Compared with the model group, 293 differential genes were screened in the MWP group, including 179 up-regulated genes and 114 down-regulated genes. Gene Ontology (GO) categories and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis indicated 17 key targets about differentiation and proliferation of neural stem cells, including Notch pathway, Rap1/B-Raf/ERK pathway and related target proteins; 9 key targets about neural endocrine-immune (NEI) network, including follicle stimulating hormone (FSH), luteinizing hormone (LH), prolactin, thyroid stimulating hormone (TSH), etc. CONCLUSION: The action mechanisms of MWP on brain in SAMP8 mouse involve the regulation of proliferation and differentiation of neural stem cells and NEI network.

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