ABSTRACT
To investigate the alkaloid constituents from the trunk barks of Winchia calophylla. The constituents were isolated and purified by column chromatography over silica gel and Sephadex LH-20 columns. The structure of the isolated compound was elucidated by MS and NMR spectra, and then confirmed by a single-crystal X-ray crystallographic analysis. The isolated compound was also evaluated for its in vitro cytotoxicity against the proliferation of seven kinds of human cancer cell lines (lung cancer: A549, breast cancer: MCF-7, prostate cancer: PC-3, glioma: U87MG, multiple myeloma: U266, MM1. S, and MM1. R). A new monoterpenoid indole alkaloid, identified as calophyline B (1), was isolated from the ethanol extract of the trunk barks of W. calophylla. Compound 1 is a new compound, which shows no cytotoxicity against the proliferation of the above human tumor cells.
ABSTRACT
Rhetsinine has been isolated from the fruits of Evodia rutaecarpa ( Juss. ) Benth. ~1H NMR and ~(13)C NMR assignments reported previously for rhetsinine were revised on the basis of UV, IR, ESI-MS,~1H NMR, ~(13)C NMR and X-ray crystallographic analysis.