ABSTRACT
Objective:To investigate the inhibitory effect and mechanism of different doses of Xiao Chai Hu Tang on C6 glioma cells cultured in vitro. Methods:C6 glioma cells were inoculated in 96 holes,24 holes and 6 holes,each culture plate was divided into 4 groups:control group, low dose group, middle dose group and high dose group, when the cells covered the bottom of culture plate 80%-90%,began adding,cultured for 24 hours after the ter mination of training. Cell proliferation activity,cell viability,protein content and protein positive expression intensity of VEGF and ESM-1,cell apoptosis in early and late stage were detected by CCK-8,in vivo staining,ELISA, immunocytochemistry and flow cytometry. Results: CCK-8 assay showed that the growth of C6 glioma cells was inhibited by low,medium and high dose group;ELISA and immunocytochemistry showed that the expression of VEGF and ESM-1 was lower in the lower, middle and high dose groups, and the levels of protein expression and protein levels were decreased. The flow cytometry showed that the low dose of small radix,middle and high dose group could promote the cell apoptosis. Inverted microscope ob-servation showed that with the increase of dose,the number of cells increased gradually,and the number of dead cells increased,and all kinds of detection methods showed that the inhibition effect increased with dose and dose dependence. The difference between the two groups was statistically significant(P<0. 01). Conclusion:The growth of C6 glioma cells was significantly inhibited by Xiao Chai Hu Tang. It may play a role in inhibiting tumor growth by down regulating ESM-1 and VEGF protein level and promoting cell apoptosis.
ABSTRACT
The Shaoyang lies next to the Interior of the Taiyang and to the Exterior of the Yangming, and is located in the half exterior and half interior position, containing both the parts and its physiological function which belong to the Gallbladder Channel of Foot-Shaoyang and the Triple Warmer Channel of Hand-Shaoyang. The Gallbladder Channel of Foot-Shaoyang controls the dispersing and discharging function of the vital energy (qi), and the Triple Warmer Channel of Hand-Shaoyang controls the function which distributes and transports the vital energy (qi), the fire and the water. When the wind-cold pathogen enters Shaoyang, anti-pathogenic vital-qi and exopathogen conflict each other, the distribution and the transport of the vital energy (qi), the fire and the water in the Triple Warmer Channel is obstructed due to the impairment of the dispersing and discharging function of the Gallbladder Channel of Foot-Shaoyang, the malfunction of Shaoyang pivots is then occured and results in the Shaoyang disease which is characteristic of the symptom complex such as bitter taste in the mouth, dryness of the throat, blurring of vision, alternate fever and chills, fullness in the chest and hypochondrium, hypochondria and anorexia, restless in mind, incessant vomiting, wiry and thready pulse and so on. This syndrome is treated by using Xiao chai fu tang which can harmonize Shaoyang. If various inproper treatments are given repeatedly to the Shaoyang disease, the syndrome to which Xiao chai hu tang must be given disappears, and a delirium as a deteriorated case appears newly, it is important to make clear what kind of inproper treatments were given, and the most appropriate treatment must be done in accordance with different present syndromes.
ABSTRACT
AIM:To investigate the effects of Fu Fang Xiao Chai Hu Tang(FFXCHT)on level of Interleukin-2 and value of CD4+/CD8+ in mice bearing Ehrlish ascites carcinoma(EAC).METHODS:The effects of FFXCHT on the EAC were observed and index of thymus and spleen were observed.The method of [3H]-TdR incorporation was used to measure the IL-2 level,and the value of CD4+/CD8+ was assayed by ELITE calibur flow cytometry.RESULTS:Compared with the model group,FFXCHT inhibited the growth of EAC(P
ABSTRACT
Female patients suffering from gallbladder stone disease were administered Sho-saiko-to (Xiao-Chai-Hu-Tang), Gorei-san (Wu-Ling-San) or Toki-shakuyaku-san (Dang-Gui-Shao-Yao-San) preoperatively, and were examined by cholangiomanometry during operation. Perfusion pressure was significantly elevated, when Sho-saiko-to or Gorei-san were administered, meaning that the pressure threshold of the sphincter of Oddi for volume load in the bile duct was lowered. This phenomenon tended to be more obvious in Gorei-san group, and will prevent duodenal fluid from transpapillary reflux. Parameters concerning the declining curve (T<sub>1/2</sub>, T<sub>1/4</sub>, T<sub>1/5</sub>) showed a significantly rapid relaxation of the sphincter of Oddi only in Sho-saiko-to group, which will result in a prevention of stasis of bile. These modulating functions of Sho-saiko-to and Gorei-san for the sphincter of Oddi would be one of the main reasons why these formulas are used for hypochondriac fullness and distress or excessively accumulated intestinal fluid. Toki-shakuyaku-san showed no such effects on the sphincter of Oddi.
ABSTRACT
Xiao Chai Hu Tang (XCHT), a blended decoction of multiple chinese medicinal herbs, was tested in combination with radiation therapy for moderate or advanced squamous carcinoma of the esophagus. A total of 66 such patients were randomized into a combined group (33 patients, XCHT + radiotherapy) and a radiation only group (33 patients, RT). Telecobalt irradiation, D T 40～70 Gy, was given to the RT group. Similar telecobalt, D T 40～68 Gy with concomitant XCHT, I dose po/day to a total of 28～50 doses, was given to the combined group. The grades Ⅰ+Ⅱresponse rate of the combined group were higher than that of the RT group. The 1-, 3-and 5 year survival rates of the combined group (69.7%, 40.9%, 25.0%) also surpassed those of the RT group (45.5%, 14.3%, 9.1%). There was statistical significance in the differences in the 1-and 3-years survival rates between the combined and RT groupsFurthermore, XCHT was able to alleviate some of the toxic reactions of radiotherapy for esophageal carcinoma.