ABSTRACT
Objective To observe the effect of Xiaoyu Huatan Decoction (XHD) on hepatic apoptosis and apoptosis- related gene Bcl-2,Bax expression in nonalcoholic fatty liver disease(NAFLD)rats,and to explore its therapeutic ef- fect for NAFLD.Methods The rat model of NAFLD was induced by feeding high fat forage.Dongbao Gantai was used as the positive control drug.Flow cytometry(FCM)was used to detect the hepatic apoptosis and expression of Bcl-2 and Bax,and optical microscope was used to observe the degree of hepatic steatosis.Results Moderate or serious steatosis of liver cells was tound under optical microscope,and there existed a few hepatic necrosis.The apoptotic rate and FI expres- sion of Bax in the model group were significantly increased (P
ABSTRACT
AIM: To observe the effect of Xiaoyu Huatan Decoction on the nonalchoholic fatty liver tissue peroxisome preliferator-activated receptor?(PPAR?) expression. METHODS: The nonalchoholic fatty liver disease rat model was fed by high fat forage and the rats were divided into five groups: normal control group,model control group,high-dose of Xiaoyu Huatan Decoction group,low-dose of Xiaoyu Huatan Decoction group,Dongbao Gantai control group.Total RNA of liver was extracted,and the expression of PPAR?mRNA was analyzed by semi-quantitative RT-PCR method.We determined the contents of total cholesterol(TC),triglyceride(TG),free ratty acid(FFA) in serum and the TC,TG in liver tissue homogenate of each group,and the degree of hepatocytic steatosis. RESULTS: Expression of liver tissue PPAR? mRNA in the model group decreased significantly,lipid in blood serum and hepatic tissues increased significantly,liver fat cell greatly denaturalized.After the intervention of medicine,expression of liver tissue PPAR? mRNA of each treatment group increased significantly,lipid in blood serum and hepatic tissues decreased significantly,liver fat cell denaturation was improved. CONCLUSION: Xiaoyu Huatan Decoction can increase the expression of liver tissue PPAR?mRNA of rats,It is likely to be one of the important mechanism for treating fatty liver.