ABSTRACT
Objective To analyze the selective extraction effect of six various cyclodextrins (α-cyclodextrin, β-cyclodextrin, methylated-β-cyclodextrin, hydroxypropyl-β-cyclodextrin, sulfobutyl ether-β-cyclodextrin, and carboxymethyl-β-cyclodextrin) on components in Chinese patent medicine Compound Xuezhining (XZN), and to establish functional equation of selective extraction effect of cyclodextrin on different chemical ingredients. Methods The chemical ingredients in each cyclodextrin extract of XZN were detected by ultrahigh-performance liquid chromatography (UPLC) fingerprints, principal component analysis (PCA) was employed to analyze the peak areas of known chemical ingredients in all samples. The structural parameters of the known components in XZN were calculated and inquired using computer aided analysis to analyze the selective extraction effect of various cyclodextrins on components. 1stOpt pro1.5 was applied to establish functional equation of selective extraction effect of cyclodextrin on chemical ingredients with different structures. Results Different extracts of XZN were distinguished effectively via PCA, and most discriminant variables for different extraction methods were obtained simultaneously. Various cyclodextrins have similar trends of selective extraction effect on components, and have different extraction effects for molecule with different properties. The functional equation between the extraction capacity of various cyclodextrins and the structural properties of the constituents was established, and the correlation coefficient is good (r > 0.86). Conclusion Different types of cyclodextrins have a selective extraction effect on the compound in XZN, which provides a theoretical basis for the selection and application of cyclodextrin and its derivatives in traditional Chinese medicine.
ABSTRACT
Objective To study the content change of six main active ingredients (orange diclofenac, rubrofusarin-6-O-β-D- gentiobioside, emodin, stilbene glucoside, nuciferine, and quercetin) in the aqueous extract under different compatibility conditions of Compound Xuezhining (CX), using Kohonen maps model for data analysis of traditional Chinese medicine with obvious change in content, to further explore how the compatibility of TCM affect the dissolution characteristic of ingredient. Methods Through different combinations of different herbs in CX, the solubility of the six active ingredients was investigated by UPLC method before and after different compatibility modes; Analyzed the data and chosen Nelumbinis Folium which has significant quantity changes after compatibility as the object of research. The impacts of different compatibility modes on seven chemical compositions of Nelumbinis Folium were analyzed by supervised Kohonen maps model. Results The content change of six active ingredients after compatibility were different: the contents of nuciferine, orange diclofenac and emodin were mainly decreased, the content of stilbene glucoside was decreased, the content of quercetin was increased, and the content of rubrofusarin-6-O-β-D-gentiobioside showed no obvious regularity; When combined with Fructus Crataegi Pinnatifidae, seven chemical constituents in Nelumbinis Folium changed greatly and most of which were positively affected. Conclusion The effects of different compatibility modes on the solubility of active components are different in Chinese herbal formulae CX, and the content of chemical constituents in Nelumbinis Folium are more influential when combined with Fructus Crataegi Pinnatifidae.